{"id":98577,"date":"2026-01-03T11:00:00","date_gmt":"2026-01-03T03:00:00","guid":{"rendered":"https:\/\/machinedquartz.com\/thermostatted-cuvettes-dna-melting-enzyme-kinetics\/"},"modified":"2026-06-12T08:10:30","modified_gmt":"2026-06-12T00:10:30","slug":"thermostatted-cuvettes-dna-melting-enzyme-kinetics","status":"publish","type":"post","link":"https:\/\/machinedquartz.com\/zh-hant\/thermostatted-cuvettes-dna-melting-enzyme-kinetics\/","title":{"rendered":"\u7528\u65bc DNA \u7194\u89e3\u8207\u9175\u7d20\u52d5\u529b\u5b78\u7684\u6046\u6eab\u6bd4\u8272\u76bf"},"content":{"rendered":"\n<div class=\"cq-aidef\" style=\"background:#fafbff;border:1px solid #e0e7ff;border-radius:10px;padding:18px 22px;margin:0 0 24px;\"><p style=\"margin:0;font-size:16px;line-height:1.65;color:#1e293b;\"><strong style=\"color:#1a2a6c;\">\u6046\u6eab\u6bd4\u8272\u76bf\u662f<\/strong> \u4e00\u652f\u5bc6\u5c01\u7684\u77f3\u82f1\u6bd4\u8272\u76bf\uff0c\u8a2d\u8a08\u5728 UV-Vis \u6216\u87a2\u5149\u91cf\u6e2c\u904e\u7a0b\u4e2d\u628a\u6a23\u54c1\u7dad\u6301\u5728\u7cbe\u78ba\u63a7\u5236\u7684\u6eab\u5ea6\uff08\u00b10.1 \u00b0C\uff09\uff0c\u7528\u65bc DNA \u7194\u89e3\u66f2\u7dda\uff0840\u201395 \u00b0C\uff09\u3001\u9175\u7d20\u52d5\u529b\u5b78\uff084\u201360 \u00b0C\uff09\u8207\u86cb\u767d\u8cea\u8b8a\u6027\u7814\u7a76\u3002\u6bd4\u8272\u76bf\u88dd\u5728 Peltier \u51b7\u537b\u6216\u6c34\u593e\u5957\u7684\u5ea7\u88e1\uff1b\u87ba\u65cb\u84cb\u9632\u6b62\u84b8\u767c\uff0c\u800c\u71b1\u654f\u96fb\u963b\u57e0\u8b93\u5100\u5668\u76f4\u63a5\u8b80\u53d6\u6a23\u54c1\u6eab\u5ea6\u3001\u800c\u975e\u4f9d\u8cf4\u5ea7\u7684\u6eab\u5ea6\u3002<\/p><\/div>\n\n\n<div class=\"csg-page\"><style>\n.csg-page { max-width:880px; margin:0 auto; padding:0 18px; color:#333; line-height:1.65; font-size:16px; }\n.csg-page p, .csg-page li { color:#333; }\n.csg-page h2 { font-size:clamp(22px,2.2vw,28px); font-weight:700; color:#1a1a2e; margin:48px 0 16px; padding-bottom:8px; 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}\n.csg-photo-card:hover { transform:translateY(-3px); box-shadow:0 8px 20px rgba(20,28,60,0.1); }\n.csg-photo-card img { width:100%; height:auto; max-width:100%; border-radius:6px; aspect-ratio:1\/1; object-fit:contain; background:#fafbfd; }\n.csg-photo-card .csg-photo-tag { display:inline-block; padding:3px 10px; border-radius:4px; background:#e6ecf8; color:#233a95; font-size:10px; font-weight:700; letter-spacing:0.6px; margin:8px 0 6px; text-transform:uppercase; }\n.csg-photo-card h4 { font-size:13px; font-weight:700; color:#1a1a2e; margin:4px 0 4px; line-height:1.3; }\n.csg-photo-card p { font-size:11px; color:#666; margin:0 0 8px; line-height:1.45; }\n.csg-photo-card a.csg-sku-link { font-size:11px; color:#233a95; font-weight:600; text-decoration:none; }\n.csg-photo-card a.csg-sku-link:hover { color:#1a2a6c; text-decoration:underline; }\n@media (max-width:720px) { .csg-photo-strip { grid-template-columns:1fr; } }\n.csg-method-grid { display:grid; grid-template-columns:repeat(3,1fr); gap:14px; margin:22px 0; }\n.csg-method-card { background:#f7f8fc; border-radius:10px; padding:18px 20px; border-left:4px solid #233a95; transition:background 0.2s, transform 0.15s; }\n.csg-method-card:hover { background:#233a95; transform:translateY(-3px); }\n.csg-method-card h4 { color:#1a1a2e; transition:color 0.2s; margin:0 0 6px; font-size:16px; }\n.csg-method-card:hover h4 { color:#fff; }\n.csg-method-card p { color:#555; transition:color 0.2s; font-size:13px; line-height:1.55; margin:0; }\n.csg-method-card:hover p { color:rgba(255,255,255,0.92); }\n.csg-method-card .csg-method-tag { display:inline-block; padding:2px 9px; border-radius:4px; background:#e6ecf8; color:#233a95; font-size:11px; font-weight:700; letter-spacing:0.6px; margin-bottom:8px; }\n.csg-method-card:hover .csg-method-tag { background:rgba(255,255,255,0.18); color:#fff; }\n@media (max-width:720px) { .csg-method-grid { grid-template-columns:1fr; } }\n.csg-related-grid { display:grid; grid-template-columns:repeat(2,1fr); gap:14px; margin:24px 0; }\n.csg-related-card { background:#fff; border:1px solid #e0e4ef; border-radius:10px; padding:16px 18px; text-decoration:none !important; color:#333 !important; transition:background 0.18s, border-color 0.18s, transform 0.15s, box-shadow 0.18s; display:block; }\n.csg-related-card:hover { background:#f7f8fc; border-color:#233a95; transform:translateY(-2px); box-shadow:0 6px 18px rgba(35,58,149,0.10); }\n.csg-related-icon { font-size:24px; line-height:1; display:block; margin-bottom:6px; }\n.csg-related-h { display:block; font-size:15px; font-weight:700; color:#1a2a6c !important; margin-bottom:4px; }\n.csg-related-d { display:block; font-size:13px; color:#555; line-height:1.45; }\n@media (max-width:720px) { .csg-related-grid { grid-template-columns:1fr; } }\n<\/style><section class=\"csg-hero\">\n<div class=\"csg-hero-eyebrow\">\u52d5\u529b\u5b78 \u00b7 \u7194\u89e3\u66f2\u7dda \u00b7 \u71b1\u7a69\u5b9a\u6027<\/div>\n<p class=\"csg-hero-h1\">\u7528\u65bc DNA \u7194\u89e3\u8207\u9175\u7d20\u52d5\u529b\u5b78\u7684\u6046\u6eab\u6bd4\u8272\u76bf<\/p>\n<p class=\"csg-hero-dek\">\u63a7\u6eab UV-Vis \u7684\u6bd4\u8272\u76bf\u9078\u64c7\uff1a\u54ea\u4e9b\u6bd4\u8272\u76bf\u5bc6\u5c01\u80fd\u5728 60 \u5206\u9418\u52d5\u529b\u5b78\u904b\u884c\u4e2d\u9632\u84b8\u767c\u3001\u54ea\u4e9b\u88dd\u5f97\u9032 Peltier \u8207\u6c34\u593e\u5957\u5ea7\u3001\u54ea\u4e9b\u9069\u5408 25 \u5230 95 \u00b0C \u7684 DNA \u7194\u89e3\uff0c\u4ee5\u53ca\u54ea\u4e9b\u8b93\u4f60\u80fd\u505a\u505c\u6d41\u800c\u4e0d\u5728\u6bcf\u6b21\u767c\u5c04\u4e4b\u9593\u5931\u53bb\u5c0d\u6e96\u3002<\/p>\n<div class=\"csg-stats\">\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">25\u201395 \u00b0C<\/div><div class=\"csg-stat-lbl\">DNA \u7194\u89e3\u7bc4\u570d<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">3<\/div><div class=\"csg-stat-lbl\">\u5bc6\u5c01\u5c64\u7d1a<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">233+<\/div><div class=\"csg-stat-lbl\">\u76f8\u5bb9\u7684 MQ SKU<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">\u00b10.1 \u00b0C<\/div><div class=\"csg-stat-lbl\">Peltier \u7a69\u5b9a\u6027<\/div><\/div>\n<\/div>\n<\/section><aside class=\"csg-toc-floating\" id=\"csg-toc\">\n<button class=\"csg-toc-handle\" type=\"button\" aria-label=\"Toggle table of contents\" onclick=\"document.getElementById('csg-toc').classList.toggle('collapsed')\"><span class=\"csg-toc-arrow-wrap\"><span class=\"csg-toc-arrow\">\u25b8<\/span><\/span><span class=\"csg-toc-vlabel\">\u672c\u9801\u5167\u5bb9<\/span><\/button>\n<div class=\"csg-toc-content\">\n<div class=\"csg-toc-title\">\u672c\u9801\u5167\u5bb9<\/div>\n<ol>\n<li><a href=\"#why\">\u70ba\u4f55\u6eab\u5ea6\u91cd\u8981<\/a><\/li>\n<li><a href=\"#methods\">3 \u7a2e\u63a7\u6eab\u65b9\u6cd5<\/a><\/li>\n<li><a href=\"#seals\">3 \u7a2e\u5bc6\u5c01\u5c64\u7d1a<\/a><\/li>\n<li><a href=\"#melting\">DNA \u7194\u89e3\u66f2\u7dda<\/a><\/li>\n<li><a href=\"#kinetics\">\u9175\u7d20\u52d5\u529b\u5b78<\/a><\/li>\n<li><a href=\"#stoppedflow\">\u505c\u6d41<\/a><\/li>\n<li><a href=\"#thermal\">\u71b1\u97ff\u61c9\u8207\u5747\u52fb\u5ea6<\/a><\/li>\n<li><a href=\"#decision\">\u6c7a\u7b56\u77e9\u9663<\/a><\/li>\n<li><a href=\"#skus\">\u4f9d\u985e\u5225\u5206\u7684 SKU<\/a><\/li>\n<li><a href=\"#tools\">\u76f8\u95dc\u6307\u5357<\/a><\/li>\n<li><a href=\"#faq\">\u5e38\u898b\u554f\u984c<\/a><\/li>\n<li><a href=\"#disclaimer\">\u514d\u8cac\u8072\u660e<\/a><\/li>\n<\/ol>\n<\/div>\n<\/aside><p>\u6eab\u5ea6\u662f UV-Vis \u91cf\u6e2c\u4e2d\u50c5\u6b21\u65bc\u5149\u7a0b\u3001\u7b2c\u4e8c\u91cd\u8981\u7684\u8b8a\u6578\uff0c\u4e5f\u662f\u6700\u5e38\u9ed8\u9ed8\u51fa\u932f\u7684\u8b8a\u6578\u3002\u6a23\u54c1\u6eab\u5ea6\u6f02\u79fb 5 \u00b0C\uff0c\u56e0\u6298\u5c04\u7387\u3001\u5bc6\u5ea6\u8207\u5206\u5b50\u69cb\u8c61\u6548\u61c9\uff0c\u6703\u8b93\u5178\u578b\u5f85\u6e2c\u7269\u7684\u6717\u4f2f-\u6bd4\u723e\u5438\u5149\u5ea6\u8b8a\u52d5 0.5\u20132%\u3002\u5c0d\u5ba4\u6eab\u4e0b\u7684\u4f8b\u884c\u7d42\u9ede\u91cf\u6e2c\uff0c\u9019\u53ef\u5ffd\u7565\u3002\u5c0d\u6574\u500b\u5be6\u9a57\u8a0a\u865f <em>\u5c31\u662f<\/em> \u4e00\u6b21\u6eab\u5ea6\u6383\u63cf\u7684 DNA \u7194\u89e3\u66f2\u7dda\u3001\u53cd\u61c9\u901f\u7387\u6bcf 10 \u00b0C \u7ffb\u500d\u7684\u9175\u7d20\u52d5\u529b\u5b78\uff0c\u6216\u4f60\u5728\u627e\u6b21\u5ea6\u7d1a\u8f49\u8b8a\u7684\u71b1\u7a69\u5b9a\u6027\u5de5\u4f5c\uff0c\u6eab\u5ea6\u63a7\u5236\u5c31\u662f\u4e00\u5207\u2014\u2014\u800c\u6bd4\u8272\u76bf\u662f\u6eab\u5ea6\u63a7\u5236\u8ff4\u8def\u7684\u4e00\u90e8\u5206\u3002<\/p>\n\n<p>\u672c\u6307\u5357\u6db5\u84cb\u662f\u4ec0\u9ebc\u8b93\u4e00\u652f\u6bd4\u8272\u76bf\u9069\u5408\u63a7\u6eab\u5de5\u4f5c\uff1a\u54ea\u4e9b\u5bc6\u5c01\u578b\u5f0f\u80fd\u5728\u9577\u52d5\u529b\u5b78\u904b\u884c\u4e2d\u9632\u84b8\u767c\u3001\u54ea\u4e9b\u898f\u683c\u88dd\u5f97\u9032 Peltier \u8207\u6c34\u593e\u5957\u6046\u6eab\u5ea7\u3001\u4ec0\u9ebc\u58c1\u539a\u8207\u6750\u6599\u7b49\u7d1a\u5f71\u97ff\u71b1\u97ff\u61c9\u6642\u9593\uff0c\u4ee5\u53ca\u54ea\u4e9b MachinedQuartz \u578b\u9304\u985e\u5225\u662f\u7194\u89e3\u66f2\u7dda\u3001\u52d5\u529b\u5b78\u8207\u505c\u6d41\u5de5\u4f5c\u6d41\u7a0b\u7684\u5c0d\u7684\u5165\u53e3\u3002\u7b2c 8 \u7bc0\u7684\u6c7a\u7b56\u77e9\u9663\u628a\u516b\u7a2e\u5e38\u898b\u7684\u63a7\u6eab\u5be6\u9a57\u985e\u578b\u5c0d\u61c9\u5230\u7279\u5b9a\u7684 MQ \u7522\u54c1\u985e\u5225\u3002<\/p>\n\n<div class=\"csg-eeat-box\">\n<strong>\u672c\u6307\u5357\u7684\u524d\u63d0\u5047\u8a2d\u3002<\/strong> \u4f60\u6709\u4e00\u53f0\u81f3\u5c11\u5e36\u4e00\u7a2e\u63a7\u6eab\u7684 UV-Vis \u6216\u87a2\u5149\u5206\u5149\u5149\u5ea6\u8a08\uff1aPeltier \u6bd4\u8272\u76bf\u5ea7\u3001\u6c34\u593e\u5957\u6bd4\u8272\u76bf\u5ea7\uff0c\u6216\u52a0\u71b1\u584a\u3002\u591a\u6578\u73fe\u4ee3\u684c\u4e0a\u578b\u5149\u8b5c\u5100\uff08Cary 60\u3001Cary 3500\u3001Lambda 365\u3001Shimadzu UV-1900\u3001JASCO V-770\uff09\u628a Peltier \u5217\u70ba\u6a19\u6e96\u6216\u4f4e\u6210\u672c\u914d\u4ef6\u3002\u6c34\u593e\u5957\u5ea7\u5e38\u898b\u65bc\u820a\u578b\u5100\u5668\u3001\u4ee5\u53ca\u9700\u8981\u66f4\u5bec\u6eab\u5ea6\u7bc4\u570d\uff08\u221210 \u00b0C \u5230 +120 \u00b0C\uff09\u7684\u5100\u5668\u3002\u4e0b\u9762\u7684\u6bd4\u8272\u76bf\u9078\u64c7\u5c0d\u9019\u4e09\u7a2e\u505a\u6cd5\u90fd\u9069\u7528\u3002\n<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg1-t\"><title id=\"svg1-t\">\u8868\u89c0\u5438\u5149\u5ea6\u6f02\u79fb\u5c0d\u6eab\u5ea6\uff0c\u986f\u793a\u5178\u578b\u5f85\u6e2c\u7269\u5438\u5149\u5ea6\u56e0\u6298\u5c04\u7387\u3001\u5bc6\u5ea6\u8207\u69cb\u8c61\u6548\u61c9\u6bcf 10 \u651d\u6c0f\u5ea6\u7d04\u8b8a\u5316 1%\uff0c\u4e26\u6709\u672a\u63a7\u8207\u53d7\u63a7\u5340\u7684\u9670\u5f71<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u8868\u89c0\u5438\u5149\u5ea6 vs \u6eab\u5ea6 \u2014 \u70ba\u4f55\u63a7\u6eab\u91cd\u8981<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">\u5178\u578b\u5f85\u6e2c\u7269\uff1a\u56e0\u6298\u5c04\u7387\u3001\u5bc6\u5ea6\u3001\u69cb\u8c61\uff0c\u6bcf 10 \u00b0C \u5438\u5149\u5ea6\u504f\u79fb\u7d04 0.5\u20132%<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"240\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><rect x=\"80\" y=\"60\" width=\"240\" height=\"240\" fill=\"#dc2626\" opacity=\"0.06\"\/><rect x=\"320\" y=\"60\" width=\"120\" height=\"240\" fill=\"#16a34a\" opacity=\"0.10\"\/><rect x=\"440\" y=\"60\" width=\"240\" height=\"240\" fill=\"#dc2626\" opacity=\"0.06\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"120\" x2=\"680\" y2=\"120\"\/><line x1=\"80\" y1=\"180\" x2=\"680\" y2=\"180\"\/><line x1=\"80\" y1=\"240\" x2=\"680\" y2=\"240\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"300\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"300\"\/><\/g><line x1=\"80\" y1=\"300\" x2=\"680\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"316\" text-anchor=\"middle\">5<\/text><text x=\"200\" y=\"316\" text-anchor=\"middle\">15<\/text><text x=\"320\" y=\"316\" text-anchor=\"middle\">25<\/text><text x=\"440\" y=\"316\" text-anchor=\"middle\">37<\/text><text x=\"560\" y=\"316\" text-anchor=\"middle\">60<\/text><text x=\"680\" y=\"316\" text-anchor=\"middle\">95<\/text><\/g><text x=\"380\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u6a23\u54c1\u6eab\u5ea6\uff08\u00b0C\uff09<\/text><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"74\" y=\"64\" text-anchor=\"end\">+8%<\/text><text x=\"74\" y=\"124\" text-anchor=\"end\">+4%<\/text><text x=\"74\" y=\"184\" text-anchor=\"end\">0<\/text><text x=\"74\" y=\"244\" text-anchor=\"end\">-4%<\/text><text x=\"74\" y=\"304\" text-anchor=\"end\">-8%<\/text><\/g><text x=\"36\" y=\"180\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 180)\">\u76f8\u5c0d 25 \u00b0C \u53c3\u8003\u7684\u8868\u89c0 A<\/text><text x=\"380\" y=\"78\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#15803d\" font-weight=\"700\">\u53d7\u63a7\u7a97\u53e3\uff08Peltier\uff09<\/text><line x1=\"80\" y1=\"220\" x2=\"200\" y2=\"200\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><line x1=\"200\" y1=\"200\" x2=\"320\" y2=\"180\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><line x1=\"320\" y1=\"180\" x2=\"440\" y2=\"160\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><line x1=\"440\" y1=\"160\" x2=\"560\" y2=\"120\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><line x1=\"560\" y1=\"120\" x2=\"680\" y2=\"80\" stroke=\"#dc2626\" stroke-width=\"2.4\" fill=\"none\"\/><g><circle cx=\"80\" cy=\"220\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"200\" cy=\"200\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"320\" cy=\"180\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"440\" cy=\"160\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"560\" cy=\"120\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"680\" cy=\"80\" r=\"4\" fill=\"#dc2626\"\/><\/g><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\" stroke=\"#16a34a\" stroke-width=\"1\" stroke-dasharray=\"4 3\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\" stroke=\"#16a34a\" stroke-width=\"1\" stroke-dasharray=\"4 3\"\/><text x=\"200\" y=\"270\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#dc2626\" font-weight=\"700\">\u51b7\u5be6\u9a57\u5ba4<\/text><text x=\"600\" y=\"270\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#dc2626\" font-weight=\"700\">\u86cb\u767d\u8cea\u8b8a\u6027 \/ \u52d5\u529b\u5b78\u52a0\u901f<\/text><\/svg><figcaption>\u5716 1 \u2014 \u5178\u578b\u5f85\u6e2c\u7269\u7684\u8868\u89c0\u5438\u5149\u5ea6\u5c0d\u6a23\u54c1\u6eab\u5ea6\u3002\u7da0\u8272\u9670\u5f71\u5e36\u662f\u53d7\u63a7\u6eab\u5ea6\u7a97\u53e3\uff08\u901a\u5e38 25 \u00b0C\u3001\u00b10.1 \u00b0C Peltier \u7a69\u5b9a\u6027\uff0c\u6216\u751f\u7269\u5de5\u4f5c\u7684 37 \u00b0C\uff09\u3002\u5728\u9019\u689d\u5e36\u4e4b\u5916\uff0c\u6298\u5c04\u7387\u8b8a\u5316\u3001\u6a23\u54c1\u5bc6\u5ea6\u8b8a\u5316\uff0c\u4ee5\u53ca\u9ad8\u6eab\u4e0b\u7684\u86cb\u767d\u8cea\u8b8a\u6027\u958b\u59cb\u4e3b\u5bb0\u91cf\u6e2c\u2014\u2014\u770b\u8d77\u4f86\u50cf\u5438\u5149\u5ea6\u8da8\u52e2\u7684\uff0c\u53ef\u80fd\u662f\u507d\u88dd\u6210\u7684\u6eab\u5ea6\u8da8\u52e2\u3002<\/figcaption><\/figure><h2 id=\"why\">1. \u70ba\u4f55\u6eab\u5ea6\u5728 UV-Vis \u91cf\u6e2c\u4e2d\u91cd\u8981<\/h2>\n<p>\u4e09\u500b\u7269\u7406\u6548\u61c9\u628a\u6a23\u54c1\u6eab\u5ea6\u8207\u8868\u89c0\u5438\u5149\u5ea6\u9023\u5728\u4e00\u8d77\uff1a<\/p>\n<ul>\n<li><strong>\u6298\u5c04\u7387\uff1a<\/strong> \u6c34\u7684\u6298\u5c04\u7387\u6bcf \u00b0C \u4e0b\u964d 0.0001\uff1b\u9019\u6539\u8b8a\u6bd4\u8272\u76bf\u7a97\u53e3\u7684\u83f2\u6d85\u8033\u53cd\u5c04\u3001\u4f7f\u8868\u89c0\u5438\u5149\u5ea6\u6bcf \u00b0C \u504f\u79fb 0.05\u20130.1%\u3002<\/li>\n<li><strong>\u6a23\u54c1\u5bc6\u5ea6\uff1a<\/strong> \u6c34\u5728 4 \u00b0C \u4ee5\u4e0a\u6bcf \u00b0C \u81a8\u8139\u7d04 0.025%\u3002mol\/L \u7684\u6fc3\u5ea6\u96a8\u6eab\u5ea6\u4e0b\u964d\uff1b\u4f60\u6bd4\u8272\u76bf\u88e1 50 \u00b0C \u7684\u6fc3\u5ea6\u6bd4\u540c\u6a23\u54c1\u5728 20 \u00b0C \u7d04\u5c11 1%\u3002<\/li>\n<li><strong>\u5206\u5b50\u69cb\u8c61\uff1a<\/strong> \u86cb\u767d\u8cea\u8207\u6838\u9178\u96a8\u6eab\u5ea6\u6539\u8b8a\u69cb\u8c61\u30021 \u00b0C \u7684\u504f\u79fb\u53ef\u900f\u904e\u8272\u80fa\u9178\u5fae\u74b0\u5883\u7684\u8b8a\u5316\uff0c\u628a\u8272\u80fa\u9178 A280 \u8b80\u6578\u79fb\u52d5 0.5%\u3002<\/li>\n<\/ul>\n<p>\u5178\u578b\u751f\u7269\u6a23\u54c1\u7684\u7e3d\u6548\u61c9\uff1a <strong>\u6bcf 10 \u00b0C \u8868\u89c0\u5438\u5149\u5ea6\u8b8a\u5316 0.5\u20132%<\/strong>\u3002\u5c0d\u8b80 0.5 AU\u3001\u5831\u5230\u5169\u4f4d\u6709\u6548\u6578\u5b57\u7684\u4f8b\u884c\u7d42\u9ede\u91cf\u6e2c\uff0c\u9019\u662f\u770b\u4e0d\u898b\u7684\u3002\u5c0d\u4f9d\u8cf4\u6eab\u5ea6\u662f\u5df2\u77e5\u5e38\u6578\u7684\u65b9\u6cd5\u2014\u2014\u5b9a\u91cf\u88fd\u85e5\u5206\u6790\u3001GMP IPC\u3001NIST SRM \u53ef\u6eaf\u6e90\u5de5\u4f5c\u2014\u2014\u4f60\u9700\u8981\u628a\u6eab\u5ea6\u9396\u5b9a\u3002\u5c0d\u4f9d\u8cf4\u6eab\u5ea6\u662f\u5df2\u77e5\u8b8a\u6578\u7684\u65b9\u6cd5\u2014\u2014DNA \u7194\u89e3\u3001\u9175\u7d20\u52d5\u529b\u5b78\u3001\u71b1\u8b8a\u6027\u2014\u2014\u4f60\u9700\u8981\u4ee5\u6821\u6b63\u904e\u7684\u7a69\u5b9a\u6027\u6383\u63cf\u6eab\u5ea6\u3002<\/p>\n\n<div class=\"csg-callout warn\"><strong>\u672a\u63a7\u6eab\u5de5\u4f5c\u88e1\u7684\u96b1\u85cf\u504f\u5dee\u3002<\/strong> \u51ac\u5929\u5728 22 \u00b0C \u5be6\u9a57\u5ba4\u958b\u767c\u3001\u590f\u5929\u5728 26 \u00b0C \u5be6\u9a57\u5ba4\u57f7\u884c\u7684\u65b9\u6cd5\uff0c\u53ef\u80fd\u5728\u5b63\u7bc0\u4e4b\u9593\u986f\u793a 0.2\u20130.4% \u7684\u7cfb\u7d71\u6027\u504f\u5dee\u3002\u5c0d HPLC \u5b9a\u91cf\u9019\u901a\u5e38\u88ab\u5176\u4ed6\u8aa4\u5dee\u4f86\u6e90\u5438\u6536\u6389\uff1b\u5c0d\u76f4\u63a5 UV-Vis \u88fd\u85e5\u5206\u6790\uff0c\u9019\u53ef\u80fd\u662f\u4e00\u500b\u65b9\u6cd5\u9a57\u8b49\u7684\u89c0\u5bdf\u9805\u3002\u628a\u6eab\u5ea6\u9396\u5b9a\u3002<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg2-t\"><title id=\"svg2-t\">UV-Vis \u5206\u5149\u5149\u5ea6\u8a08\u7684\u4e09\u7a2e\u63a7\u6eab\u65b9\u6cd5\u4e26\u6392\u6bd4\u8f03\uff1aPeltier \u71b1\u96fb\u6bd4\u8272\u76bf\u5ea7 vs \u6c34\u593e\u5957\u6bd4\u8272\u76bf\u5ea7 vs \u52a0\u71b1\u584a\u5ea7\uff0c\u986f\u793a\u5178\u578b\u6eab\u5ea6\u7bc4\u570d\u3001\u7a69\u5b9a\u6027\u8207\u914d\u4ef6\u6210\u672c<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u63a7\u6eab\u65b9\u6cd5 \u2014 Peltier vs \u6c34\u593e\u5957 vs \u52a0\u71b1\u584a<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">\u4e09\u8005\u90fd\u63a5\u53d7\u6a19\u6e96 12.5\u00d712.5 mm \u6bd4\u8272\u76bf\uff1b\u9078\u64c7\u53d6\u6c7a\u65bc\u7bc4\u570d\u3001\u7a69\u5b9a\u6027\u8207\u9810\u7b97<\/text><g font-family=\"Arial,sans-serif\"><g transform=\"translate(20,70)\"><rect x=\"0\" y=\"0\" width=\"220\" height=\"240\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"110\" y=\"22\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#1a2a6c\">1. Peltier\uff08TEC\uff09<\/text><text x=\"110\" y=\"38\" text-anchor=\"middle\" font-size=\"10\" fill=\"#666\">\u6700\u5e38\u898b \u2014 \u73fe\u4ee3\u9810\u8a2d<\/text><rect x=\"40\" y=\"60\" width=\"140\" height=\"80\" fill=\"#fff\" stroke=\"#1a2a6c\" stroke-width=\"1\"\/><rect x=\"80\" y=\"80\" width=\"60\" height=\"40\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"0.8\"\/><rect x=\"40\" y=\"148\" width=\"140\" height=\"14\" fill=\"#dc2626\" opacity=\"0.4\"\/><text x=\"110\" y=\"160\" text-anchor=\"middle\" font-size=\"9\" fill=\"#fff\" font-weight=\"700\">TEC \u5143\u4ef6<\/text><rect x=\"40\" y=\"166\" width=\"140\" height=\"14\" fill=\"#0ea5e9\" opacity=\"0.4\"\/><text x=\"110\" y=\"178\" text-anchor=\"middle\" font-size=\"9\" fill=\"#0369a1\" font-weight=\"700\">\u6563\u71b1\u7247<\/text><text x=\"110\" y=\"206\" text-anchor=\"middle\" font-size=\"11\" fill=\"#1a1a2e\" font-weight=\"700\">5\u2013100 \u00b0C<\/text><text x=\"110\" y=\"222\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u00b10.1 \u00b0C \u7a69\u5b9a\u6027<\/text><text x=\"110\" y=\"238\" text-anchor=\"middle\" font-size=\"10\" fill=\"#16a34a\" font-weight=\"700\">$$ \u914d\u4ef6<\/text><\/g><g transform=\"translate(250,70)\"><rect x=\"0\" y=\"0\" width=\"220\" height=\"240\" fill=\"#dcfce7\" stroke=\"#16a34a\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"110\" y=\"22\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#15803d\">2. \u6c34\u593e\u5957<\/text><text x=\"110\" y=\"38\" text-anchor=\"middle\" font-size=\"10\" fill=\"#666\">\u7bc4\u570d\u6700\u5bec \u2014 \u820a\u578b \/ \u6975\u7aef\u6eab\u5ea6<\/text><rect x=\"40\" y=\"60\" width=\"140\" height=\"120\" fill=\"#fff\" stroke=\"#15803d\" stroke-width=\"1\"\/><rect x=\"80\" y=\"80\" width=\"60\" height=\"80\" fill=\"#dbeafe\" stroke=\"#15803d\" stroke-width=\"0.8\"\/><path d=\"M 40 80 L 30 80 L 30 160 L 40 160\" stroke=\"#0ea5e9\" stroke-width=\"2\" fill=\"none\"\/><path d=\"M 180 80 L 190 80 L 190 160 L 180 160\" stroke=\"#0ea5e9\" stroke-width=\"2\" fill=\"none\"\/><polygon points=\"22 110, 30 100, 30 120\" fill=\"#0ea5e9\"\/><polygon points=\"198 130, 190 120, 190 140\" fill=\"#0ea5e9\"\/><text x=\"20\" y=\"156\" font-size=\"9\" fill=\"#0369a1\" font-weight=\"700\">\u9032<\/text><text x=\"195\" y=\"156\" font-size=\"9\" fill=\"#0369a1\" font-weight=\"700\">\u51fa<\/text><text x=\"110\" y=\"206\" text-anchor=\"middle\" font-size=\"11\" fill=\"#1a1a2e\" font-weight=\"700\">\u221210 \u5230 +120 \u00b0C<\/text><text x=\"110\" y=\"222\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u00b10.05 \u00b0C \u7a69\u5b9a\u6027<\/text><text x=\"110\" y=\"238\" text-anchor=\"middle\" font-size=\"10\" fill=\"#f59e0b\" font-weight=\"700\">$ \u5ea7 + \u5faa\u74b0\u5668<\/text><\/g><g transform=\"translate(480,70)\"><rect x=\"0\" y=\"0\" width=\"220\" height=\"240\" fill=\"#fef9c3\" stroke=\"#ca8a04\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"110\" y=\"22\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#854d0e\">3. \u52a0\u71b1\u584a<\/text><text x=\"110\" y=\"38\" text-anchor=\"middle\" font-size=\"10\" fill=\"#666\">\u7279\u6b8a \u2014 OLIS\u3001Aviv\u3001\u5ba2\u88fd\u88dd\u7f6e<\/text><rect x=\"40\" y=\"60\" width=\"140\" height=\"100\" fill=\"#854d0e\" stroke=\"#ca8a04\" stroke-width=\"1\"\/><rect x=\"80\" y=\"80\" width=\"60\" height=\"60\" fill=\"#fff\" stroke=\"#ca8a04\" stroke-width=\"0.8\"\/><rect x=\"40\" y=\"160\" width=\"140\" height=\"14\" fill=\"#dc2626\" opacity=\"0.6\"\/><text x=\"110\" y=\"172\" text-anchor=\"middle\" font-size=\"9\" fill=\"#fff\" font-weight=\"700\">\u96fb\u963b\u52a0\u71b1\u5668<\/text><text x=\"110\" y=\"206\" text-anchor=\"middle\" font-size=\"11\" fill=\"#1a1a2e\" font-weight=\"700\">25\u2013200 \u00b0C<\/text><text x=\"110\" y=\"222\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u00b10.5 \u00b0C \u7a69\u5b9a\u6027<\/text><text x=\"110\" y=\"238\" text-anchor=\"middle\" font-size=\"10\" fill=\"#dc2626\" font-weight=\"700\">$$$ \u7279\u6b8a<\/text><\/g><\/g><rect x=\"40\" y=\"320\" width=\"640\" height=\"34\" fill=\"#f7f8fc\" stroke=\"#cdd2dd\" stroke-width=\"0.6\" rx=\"6\"\/><text x=\"60\" y=\"342\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#1a1a2e\">\u4e09\u7a2e\u5ea7\u578b\u90fd\u63a5\u53d7\u6a19\u6e96 12.5\u00d712.5 mm MachinedQuartz \u6bd4\u8272\u76bf\u2014\u2014\u6bd4\u8272\u76bf\u9078\u64c7\u8207\u63a7\u6eab\u65b9\u6cd5\u7121\u95dc\u3002<\/text><\/svg><figcaption>\u5716 2 \u2014 \u4e09\u7a2e\u63a7\u6eab\u67b6\u69cb\u3002Peltier \u662f 5\u2013100 \u00b0C \u5de5\u4f5c\u7684\u73fe\u4ee3\u9810\u8a2d\uff1b\u6c34\u593e\u5957\u5ef6\u4f38\u5230 \u221210 \u5230 +120 \u00b0C\uff0c\u662f\u820a\u578b\u5149\u8b5c\u5100\u8207\u6975\u7aef\u6eab\u5ea6\u5de5\u4f5c\u7684\u504f\u597d\uff1b\u52a0\u71b1\u584a\u662f\u6975\u9ad8\u6eab\u5438\u5149\u5ea6\u7814\u7a76\u7684\u7279\u6b8a\u9078\u64c7\u3002\u4e09\u8005\u90fd\u63a5\u53d7\u6a19\u6e96 MQ \u6bd4\u8272\u76bf\u3002<\/figcaption><\/figure><h2 id=\"methods\">2. \u4e09\u7a2e\u63a7\u6eab\u65b9\u6cd5<\/h2>\n<p>\u4e09\u7a2e\u4e0d\u540c\u7684\u67b6\u69cb\u57fa\u672c\u4e0a\u6db5\u84cb\u6240\u6709 UV-Vis \u63a7\u6eab\u3002\u4e09\u8005\u7684\u6bd4\u8272\u76bf\u9078\u64c7\u76f8\u540c\u3002<\/p>\n\n<div class=\"csg-method-grid\">\n<div class=\"csg-method-card\"><span class=\"csg-method-tag\">\u65b9\u6cd5 1 \u2014 \u73fe\u4ee3\u9810\u8a2d<\/span><h4>Peltier\uff08\u71b1\u96fb\uff09<\/h4><p>\u8207\u6bd4\u8272\u76bf\u5ea7\u76f4\u63a5\u63a5\u89f8\u7684\u56fa\u614b\u71b1\u96fb\u51b7\u537b\u5143\u4ef6\u3002\u9760\u53cd\u8f49\u96fb\u6d41\u65b9\u5411\u4f86\u52a0\u71b1\u6216\u51b7\u537b\u3002\u7bc4\u570d 5\u2013100 \u00b0C\uff1b\u7a69\u5b9a\u6027 \u00b10.1 \u00b0C\u3002\u662f Cary 3500\u3001Lambda 365\u3001Shimadzu UV-1900\u3001JASCO V-770 \u7684\u6a19\u6e96\u914d\u4ef6\u3002\u4e0d\u9700\u8981\u6c34\u7ba1\u3002<\/p><\/div>\n<div class=\"csg-method-card\"><span class=\"csg-method-tag\">\u65b9\u6cd5 2 \u2014 \u7bc4\u570d\u6700\u5bec<\/span><h4>\u6c34\u593e\u5957<\/h4><p>\u7a7a\u5fc3\u58c1\u5ea7\u3001\u7531\u5916\u90e8\u6c34\u6d74\u5faa\u74b0\u6c34\u3002\u914d\u9069\u7576\u6d41\u9ad4\uff08\u6c34\u3001\u4e59\u4e8c\u9187\u3001\u77fd\u6cb9\uff09\u7bc4\u570d \u221210 \u5230 +120 \u00b0C\u3002\u914d\u512a\u8cea\u5faa\u74b0\u5668\u7a69\u5b9a\u6027 \u00b10.05 \u00b0C\u3002\u662f\u820a\u578b Cary\u3001Lambda \u8207\u5c08\u7528\u52d5\u529b\u5b78\u5100\u5668\u7684\u6a19\u6e96\u3002<\/p><\/div>\n<div class=\"csg-method-card\"><span class=\"csg-method-tag\">\u65b9\u6cd5 3 \u2014 \u7279\u6b8a<\/span><h4>\u52a0\u71b1\u584a<\/h4><p>\u5d4c\u5728\u5ea7\u672c\u9ad4\u88e1\u7684\u96fb\u963b\u52a0\u71b1\u5143\u4ef6\u3002\u7bc4\u570d 25\u2013200 \u00b0C\uff1b\u7a69\u5b9a\u6027 \u00b10.5 \u00b0C\u3002\u7528\u65bc\u5c08\u7528\u9ad8\u6eab\u5100\u5668\uff08OLIS\u3001Aviv\uff09\u8207\u5ba2\u88fd\u88dd\u7f6e\u3002\u4e0d\u5c0d\u7a31\u2014\u2014\u80fd\u52a0\u71b1\u5230\u74b0\u5883\u4ee5\u4e0a\u3001\u4f46\u7121\u6cd5\u51b7\u537b\u5230\u74b0\u5883\u4ee5\u4e0b\u3002<\/p><\/div>\n<\/div>\n\n<h3>\u5728\u5b83\u5011\u4e4b\u9593\u9078\u64c7<\/h3>\n<p>\u5982\u679c\u4f60\u7684\u5149\u8b5c\u5100\u662f\u904e\u53bb 10 \u5e74\u7684\uff0c\u5b83\u5e7e\u4e4e\u4e00\u5b9a\u628a Peltier \u5217\u70ba\u73fe\u8ca8\u6216\u4f4e\u6210\u672c\u914d\u4ef6\u3002\u7528\u5b83\u3002\u5982\u679c\u4f60\u7684\u5de5\u4f5c\u9700\u8981\u96f6\u4e0b\u6eab\u5ea6\u3001\u6975\u9ad8\u6eab\uff08100 \u00b0C \u4ee5\u4e0a\uff09\uff0c\u6216\u4f60\u9700\u8981 \u00b10.05 \u00b0C \u7a69\u5b9a\u6027\u505a\u975e\u5e38\u7cbe\u78ba\u7684\u7194\u89e3\u66f2\u7dda\u5de5\u4f5c\uff0c\u6c34\u593e\u5957\u914d\u7f6e\u624d\u662f\u5c0d\u7684\u9078\u64c7\u3002\u52a0\u71b1\u584a\u4fdd\u7559\u7d66\u5c08\u70ba\u9ad8\u6eab\u5438\u5149\u5ea6\u8a2d\u8a08\u7684\u5100\u5668\u2014\u2014\u4e0d\u662f\u4f8b\u884c\u9078\u64c7\u3002<\/p>\n\n<div class=\"csg-callout tip\"><strong>\u4e09\u7a2e\u65b9\u6cd5\u90fd\u7528\u540c\u4e00\u652f\u6bd4\u8272\u76bf\u3002<\/strong> \u4e00\u652f\u6a19\u6e96 12.5 \u00d7 12.5 mm \u5916\u90e8\u5c3a\u5bf8\u7684\u6bd4\u8272\u76bf\uff0c\u88dd\u5f97\u9032\u6211\u5011\u898b\u904e\u7684\u6bcf\u4e00\u500b Peltier \u5ea7\u3001\u6bcf\u4e00\u500b\u6c34\u593e\u5957\u5ea7\u3001\u6bcf\u4e00\u500b\u52a0\u71b1\u584a\u3002\u6bd4\u8272\u76bf\u5e7e\u4f55\u4e0d\u662f\u8b8a\u6578\uff1b\u6bd4\u8272\u76bf\u7684 <em>\u5bc6\u5c01\u578b\u5f0f<\/em> \u624d\u662f\u3002<\/div><h2 id=\"seals\">3. \u4e09\u7a2e\u5bc6\u5c01\u5c64\u7d1a \u2014 \u4f9d\u5be6\u9a57\u6642\u9577\u9078<\/h2>\n<p>\u4e00\u65e6\u4f60\u5b9a\u5728\u8207\u74b0\u5883\u4e0d\u540c\u7684\u6eab\u5ea6\uff0c\u6c7a\u5b9a\u6027\u7684\u9650\u5236\u5c31\u6210\u4e86\u84b8\u767c\u300210 \u00b0C \u7684\u6eab\u5dee\u900f\u904e\u958b\u653e\u7684\u6bd4\u8272\u76bf\u9802\u90e8\u6bcf\u5c0f\u6642\u9a45\u52d5\u7d04 1% \u7684\u9ad4\u7a4d\u6d41\u5931\uff1b30 \u00b0C \u7684\u6eab\u5dee\uff0830 \u00b0C \u5be6\u9a57\u5ba4\u88e1\u7684 60 \u00b0C \u6bd4\u8272\u76bf\uff09\u6bcf\u5c0f\u6642\u5931\u7d04 3%\u3002\u6d41\u5931\u7684\u6c34\u6fc3\u7e2e\u4e86\u5f85\u6e2c\u7269\u3001\u4f7f\u91cf\u5230\u7684\u5438\u5149\u5ea6\u504f\u79fb\u540c\u6a23\u7684\u6bd4\u4f8b\u3002\u4e09\u7a2e\u5bc6\u5c01\u5c64\u7d1a\u61c9\u4ed8\u4e0d\u540c\u7684\u5be6\u9a57\u6642\u9577\u3002<\/p>\n\n<h3>\u5c64\u7d1a 1 \u2014 PTFE \u6469\u64e6\u84cb\uff08\u5e36\u84cb\u7684\u958b\u653e\u6bd4\u8272\u76bf\uff09<\/h3>\n<p>\u6a19\u6e96\u578b\u9304\u6bd4\u8272\u76bf\u51fa\u8ca8\u5373\u9644 PTFE \u58d3\u914d\u84cb\u3002\u84cb\u5b50\u76f8\u8f03\u958b\u653e\u6bd4\u8272\u76bf\u6e1b\u5c11\u7d04 60\u201370% \u7684\u84b8\u767c\u3001\u4f46\u4e0d\u505a\u6c23\u5bc6\u5bc6\u5c01\u3002\u9069\u5408\uff1a\u5ba4\u6eab\u7d42\u9ede\u91cf\u6e2c\u3001\u9069\u5ea6\u6eab\u5dee\uff08\u96e2\u74b0\u5883 \u00b115 \u00b0C\uff09\u4e0b 10 \u5206\u9418\u4ee5\u5167\u7684\u52d5\u529b\u5b78\u904b\u884c\u3001\u65b9\u6cd5\u5b9a\u7fa9\u826f\u597d\u7684\u4f8b\u884c\u88fd\u85e5\u54c1\u7ba1\u3002<\/p>\n\n<h3>\u5c64\u7d1a 2 \u2014 PTFE \u585e\uff08\u7cbe\u5bc6\u914d\u5408\u7684\u78e8\u7802\u585e\uff09<\/h3>\n<p>\u4e00\u500b\u914d\u5728\u6bd4\u8272\u76bf\u672c\u9ad4\u78e8\u7802\u73bb\u7483\u627f\u5ea7\u4e0a\u7684 PTFE \u52a0\u5de5\u585e\u300230 \u5206\u9418\u5167\u5bc6\u5c01\u8868\u73fe\u7d04 95%\uff1b\u9069\u5408\u53ef\u63a5\u53d7\u8f15\u5fae\u84b8\u767c\u4fee\u6b63\u7684 10\u201360 \u5206\u9418\u52d5\u529b\u5b78\u904b\u884c\u3002\u6bd4\u87ba\u65cb\u84cb\u66f4\u597d\u63d2\u5165\u8207\u53d6\u51fa\uff0c\u9019\u5728\u6d41\u7a0b\u9700\u8981\u5be6\u9a57\u4e2d\u591a\u6b21\u52a0\u6a23\u6642\u5f88\u8981\u7dca\u3002<\/p>\n\n<h3>\u5c64\u7d1a 3 \u2014 \u87ba\u65cb\u84cb\uff08\u5bc6\u5c01\u6bd4\u8272\u76bf\uff09<\/h3>\n<p>\u4e00\u500b\u9644 PTFE \u5167\u896f\u7684\u87ba\u7d0b\u84cb\u5f62\u6210\u57fa\u672c\u4e0a\u6c23\u5bc6\u7684\u5bc6\u5c01\u3002\u591a\u5c0f\u6642\u904b\u884c\u5bc6\u5c01\u8868\u73fe >99%\uff1b\u5c0d DNA \u7194\u89e3\uff08\u6eab\u5ea6\u6383\u63cf\u6642\u9577 30\u201390 \u5206\u9418\uff09\u3001\u9577\u65bc 60 \u5206\u9418\u7684\u9175\u7d20\u52d5\u529b\u5b78\u3001\u53ad\u6c27\u5de5\u4f5c\u8207\u4efb\u4f55\u63ee\u767c\u6eb6\u5291\u61c9\u7528\uff0c\u90fd\u662f\u5fc5\u5099\u3002\u88dd\u586b\u6642\u7565\u6709\u6642\u9593\u4ee3\u50f9\uff0c\u56e0\u70ba\u84cb\u5b50\u5fc5\u9808\u5c0f\u5fc3\u9396\u7dca\u3001\u4e0d\u56f0\u4f4f\u6c23\u6ce1\u3002<\/p>\n\n<p>\u95dc\u65bc\u6bcf\u7a2e\u5bc6\u5c01\u5982\u4f55\u904b\u4f5c\u3001\u4f55\u6642\u5728\u5b83\u5011\u4e4b\u9593\u9078\u64c7\uff0c\u4ee5\u53ca\u84cb\u5b50\u5167\u896f\u6750\u6599\u53d6\u6368\uff08PTFE \/ \u77fd\u81a0 \/ Viton \/ \u4e01\u57fa \/ EPDM\uff09\u7684\u66f4\u6df1\u5165\u8655\u7406\uff0c\u898b\u6211\u5011\u7684 <a href=\"https:\/\/machinedquartz.com\/zh-hant\/screw-top-cuvette-guide\/\">\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u6307\u5357<\/a>.<\/p><div class=\"csg-photo-strip\">\n<div class=\"csg-photo-card\">\n<img decoding=\"async\" src=\"https:\/\/machinedquartz.com\/wp-content\/uploads\/2025\/07\/C102TR8-10mm-Fluorescent-Screw-Cap-Cuvette-3.5ml-Two-way-Light-Screw-Cap-1pc-ea.jpg\" alt=\"C102TR8 10mm screw-cap quartz cuvette 3.5 millilitre two-way light with PTFE-lined screw closure for kinetic and melting curve work\" loading=\"lazy\" \/>\n<span class=\"csg-photo-tag\">\u5c64\u7d1a 3 \u00b7 \u5bc6\u5c01<\/span>\n<h4>C102TR8\u2014\u201410 mm \u87ba\u65cb\u84cb<\/h4>\n<p>3.5 mL \u00b7 \u96d9\u9762\u900f\u5149 \u00b7 DNA \u7194\u89e3\u8207\u9577\u9175\u7d20\u52d5\u529b\u5b78\u904b\u884c\u7684\u4e3b\u529b<\/p>\n<a class=\"csg-sku-link\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-screw-top-cuvettes\/\">\u67e5\u770b\u87ba\u65cb\u84cb\u7cfb\u5217 \u2192<\/a>\n<\/div>\n<div class=\"csg-photo-card\">\n<img decoding=\"async\" src=\"https:\/\/machinedquartz.com\/wp-content\/uploads\/2025\/07\/C104SE6-10mm-Cuvette-With-Stopper-3.5ml-Four-Way-Light-Stopper-Pack-of-Two.jpg\" alt=\"C104SE6 10mm stoppered fluorescence quartz cuvette 3.5 millilitre four-way light with PTFE stopper for kinetic work with intermediate sample additions\" loading=\"lazy\" \/>\n<span class=\"csg-photo-tag\">\u5c64\u7d1a 2 \u00b7 \u52a0\u585e<\/span>\n<h4>C104SE6\u2014\u201410 mm \u52a0\u585e\u87a2\u5149<\/h4>\n<p>3.5 mL \u00b7 \u56db\u900f\u660e\u9762 \u00b7 \u78e8\u7802\u585e\uff0c\u4f9b\u6709\u52a0\u8a66\u5291\u7684\u87a2\u5149\u52d5\u529b\u5b78<\/p>\n<a class=\"csg-sku-link\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-stoppered-cuvettes\/\">\u67e5\u770b\u52a0\u585e\u7cfb\u5217 \u2192<\/a>\n<\/div>\n<div class=\"csg-photo-card\">\n<img decoding=\"async\" src=\"https:\/\/machinedquartz.com\/wp-content\/uploads\/2025\/07\/C034WS-3mm-4mm-OD-Diameter-Flow-Cuvette-300ul-Four-Way-Light-1pc-ea.jpg\" alt=\"C034WS 3mm path quartz fluorescence flow cuvette 300 microlitre four-way light with 4 millimetre outer diameter inlet outlet ports for stopped-flow kinetics\" loading=\"lazy\" \/>\n<span class=\"csg-photo-tag\">\u505c\u6d41<\/span>\n<h4>C034WS\u2014\u20143 mm \u6d41\u901a\u6bd4\u8272\u76bf<\/h4>\n<p>300 \u00b5L \u00b7 \u56db\u9762\u900f\u5149 \u00b7 \u9032\/\u51fa\u53e3\u57e0\uff0c\u4f9b\u505c\u6d41\u8207\u9023\u7e8c\u6d41\u52d5\u529b\u5b78<\/p>\n<a class=\"csg-sku-link\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-flow-cells\/\">\u67e5\u770b\u6d41\u901a\u6c60 \u2192<\/a>\n<\/div>\n<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg3-t\"><title id=\"svg3-t\">DNA \u7194\u89e3\u66f2\u7dda S \u5f62\u5716\uff0c\u986f\u793a 260 \u5948\u7c73\u5438\u5149\u5ea6\u5c0d 25 \u5230 95 \u651d\u6c0f\u5ea6\u6eab\u5ea6\uff0c\u542b\u96d9\u80a1\u5230\u55ae\u80a1 DNA \u589e\u8272\u504f\u79fb\u7684\u7194\u89e3\u8f49\u8b8a\u4e2d\u9ede Tm \u6a19\u8a18<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">DNA \u7194\u89e3\u66f2\u7dda \u2014 A\u2082\u2086\u2080 vs \u6eab\u5ea6<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">dsDNA \u2192 ssDNA \u8f49\u8b8a\u7684\u589e\u8272\u504f\u79fb\uff1bTm = S \u5f62\u66f2\u7dda\u7684\u4e2d\u9ede<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"240\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"120\" x2=\"680\" y2=\"120\"\/><line x1=\"80\" y1=\"180\" x2=\"680\" y2=\"180\"\/><line x1=\"80\" y1=\"240\" x2=\"680\" y2=\"240\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"300\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"300\"\/><\/g><line x1=\"80\" y1=\"300\" x2=\"680\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"316\" text-anchor=\"middle\">25<\/text><text x=\"200\" y=\"316\" text-anchor=\"middle\">40<\/text><text x=\"320\" y=\"316\" text-anchor=\"middle\">55<\/text><text x=\"440\" y=\"316\" text-anchor=\"middle\">70<\/text><text x=\"560\" y=\"316\" text-anchor=\"middle\">85<\/text><text x=\"680\" y=\"316\" text-anchor=\"middle\">95<\/text><\/g><text x=\"380\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u6eab\u5ea6\uff08\u00b0C\uff09<\/text><text x=\"36\" y=\"180\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 180)\">260 nm \u7684\u5438\u5149\u5ea6<\/text><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"74\" y=\"64\" text-anchor=\"end\">1.30<\/text><text x=\"74\" y=\"124\" text-anchor=\"end\">1.20<\/text><text x=\"74\" y=\"184\" text-anchor=\"end\">1.10<\/text><text x=\"74\" y=\"244\" text-anchor=\"end\">1.00<\/text><text x=\"74\" y=\"304\" text-anchor=\"end\">0.95<\/text><\/g><path d=\"M 80 245 L 130 244 L 175 242 L 220 240 L 265 235 L 300 226 L 325 210 L 348 188 L 372 162 L 395 132 L 420 108 L 450 94 L 480 88 L 530 84 L 580 82 L 680 80\" stroke=\"#1a2a6c\" stroke-width=\"2.6\" fill=\"none\"\/><line x1=\"372\" y1=\"60\" x2=\"372\" y2=\"300\" stroke=\"#dc2626\" stroke-width=\"1.5\" stroke-dasharray=\"6 3\"\/><text x=\"372\" y=\"56\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#dc2626\">Tm = 65 \u00b0C<\/text><circle cx=\"372\" cy=\"162\" r=\"6\" fill=\"#dc2626\"\/><g><text x=\"180\" y=\"220\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#1a2a6c\">dsDNA \u7a69\u5b9a<\/text><text x=\"180\" y=\"234\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#666\">A\u2082\u2086\u2080 \u57fa\u7dda<\/text><text x=\"600\" y=\"78\" text-anchor=\"end\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#1a2a6c\">ssDNA<\/text><text x=\"600\" y=\"92\" text-anchor=\"end\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#666\">+30% \u589e\u8272<\/text><\/g><g transform=\"translate(450,210)\" font-family=\"Arial,sans-serif\"><rect x=\"0\" y=\"0\" width=\"200\" height=\"80\" fill=\"#fef9c3\" stroke=\"#ca8a04\" stroke-width=\"0.8\" rx=\"4\"\/><text x=\"10\" y=\"18\" font-size=\"11\" font-weight=\"700\" fill=\"#854d0e\">\u6bd4\u8272\u76bf\u9700\u6c42<\/text><text x=\"10\" y=\"34\" font-size=\"10\" fill=\"#444\">\u2022 \u5bc6\u5c01\uff08\u87ba\u65cb\u84cb\u6216\u585e\uff09<\/text><text x=\"10\" y=\"48\" font-size=\"10\" fill=\"#444\">\u2022 \u56db\u900f\u660e\u9762\u53ef\u4ee5\uff1b\u96d9\u9762 OK<\/text><text x=\"10\" y=\"62\" font-size=\"10\" fill=\"#444\">\u2022 200\u2013500 \u00b5L \u6a23\u54c1\u9ad4\u7a4d<\/text><text x=\"10\" y=\"76\" font-size=\"10\" fill=\"#444\">\u2022 PTFE \u5167\u896f\u84cb\uff08\u4e0d\u6790\u51fa\uff09<\/text><\/g><\/svg><figcaption>\u5716 3 \u2014 DNA \u7194\u89e3\u66f2\u7dda\u3002\u6eab\u5ea6\u901a\u5e38\u4ee5 0.5\u20131 \u00b0C\/min \u5f9e 25 \u6383\u5230 95 \u00b0C\u3002\u7576 dsDNA \u80a1\u5206\u96e2\uff0c\u672a\u5806\u758a\u7684\u9e7c\u57fa\u5728 260 nm \u7d66\u51fa 30\u201340% \u7684\u300c\u589e\u8272\u300d\u5438\u5149\u5ea6\u589e\u52a0\u3002S \u5f62\u66f2\u7dda\u7684\u4e2d\u9ede\u5c31\u662f\u7194\u89e3\u6eab\u5ea6 Tm\u300230\u201390 \u5206\u9418\u7684\u6383\u63cf\u6642\u9577\u8981\u6c42\u5bc6\u5c01\u6bd4\u8272\u76bf\u2014\u2014\u958b\u653e\u6bd4\u8272\u76bf\u5728\u904b\u884c\u4e2d\u84b8\u767c\u640d\u5931\u5e7e\u500b\u767e\u5206\u6bd4\u7684\u9ad4\u7a4d\uff0c\u4f7f\u7b97\u51fa\u7684 Tm \u504f\u5dee\u3002<\/figcaption><\/figure><h2 id=\"melting\">4. DNA \/ RNA \/ \u5be1\u6838\u82f7\u9178\u7194\u89e3\u66f2\u7dda<\/h2>\n<p>\u5728 260 nm \u76e3\u6e2c\u7684\u71b1\u8b8a\u6027\u662f\u63a7\u6eab UV-Vis \u7684\u6559\u79d1\u66f8\u7bc4\u4f8b\u3002\u7576\u96d9\u80a1 DNA \u7194\u6210\u55ae\u80a1\uff0c\u672a\u5806\u758a\u7684\u9e7c\u57fa\u5931\u53bb\u5b83\u5011\u7684\u6e1b\u8272\u5c4f\u853d\u3001\u5438\u5149\u5ea6\u589e\u52a0 30\u201340%\u3002\u7194\u89e3\u6eab\u5ea6 Tm\uff08S \u5f62\u66f2\u7dda\u7684\u4e2d\u9ede\uff09\u662f\u5f15\u5b50\u8a2d\u8a08\u3001\u7a81\u8b8a\u5075\u6e2c\u8207\u6838\u9178\u8868\u5fb5\u7684\u8a3a\u65b7\u8b80\u51fa\u3002<\/p>\n\n<h3>\u7194\u89e3\u5de5\u4f5c\u7684\u6bd4\u8272\u76bf\u9700\u6c42<\/h3>\n<ul>\n<li><strong>\u5bc6\u5c01\u84cb\u5fc5\u5099\u3002<\/strong> \u6383\u63cf\u5f9e 25\u201395 \u00b0C \u8dd1 30\u201390 \u5206\u9418\u3002\u958b\u653e\u6216 PTFE \u6469\u64e6\u84cb\u6bd4\u8272\u76bf\u84b8\u767c\u640d\u5931 3\u20138% \u7684\u9ad4\u7a4d\uff0c\u6fc3\u7e2e DNA \u4e26\u4f7f\u8868\u89c0 A260 \u504f\u79fb\u540c\u6a23\u7684\u500d\u7387\u3002\u7528\u87ba\u65cb\u84cb\u6216\u7cbe\u5bc6\u52a0\u585e\u6bd4\u8272\u76bf\u3002<\/li>\n<li><strong>\u5149\u7a0b 10 mm<\/strong> \u7d66\u5178\u578b 50\u2013500 ng\/\u00b5L \u7684\u7194\u89e3\u5de5\u4f5c\u3002\u5c0d\u975e\u5e38\u7a00\u7684\u6a23\u54c1\uff08< 50 ng\/\u00b5L\uff09\uff0c50 mm \u5149\u7a0b\u6216\u4ee5\u87a2\u5149\u70ba\u57fa\u790e\u7684\u5075\u6e2c\uff08\u7528\u5d4c\u5165\u67d3\u6599\uff09\u6bd4\u66f4\u9577\u7684\u6bd4\u8272\u76bf\u66f4\u5408\u7406\u3002<\/li>\n<li><strong>\u9ad4\u7a4d 200\u2013500 \u00b5L<\/strong> \u5728 10 mm \u6b21\u5fae\u91cf\u6216\u534a\u5fae\u91cf\u6bd4\u8272\u76bf\u88e1\u628a\u6a23\u54c1\u6d88\u8017\u964d\u5230\u6700\u4f4e\u3002\u5c0d 3.5 mL \u6a19\u6e96\u6bd4\u8272\u76bf\uff0c\u8f03\u5927\u7684\u6a23\u54c1\u9ad4\u7a4d\u7d66\u51fa\u7565\u597d\u7684\u71b1\u7a69\u5b9a\u6027\u3001\u4f46\u6d88\u8017\u66f4\u591a\u73cd\u8cb4\u7684\u5be1\u6838\u82f7\u9178\u3002<\/li>\n<li><strong>\u56db\u900f\u660e\u9762\u6709\u7528<\/strong> \u7d66\u4e26\u884c\u7684 A260 \u5438\u5149\u5ea6\u8207\u4ee5\u87a2\u5149\u70ba\u57fa\u790e\u7684\u7194\u89e3\uff08\u50cf SYBR Safe \u9019\u985e\u5d4c\u5165\u67d3\u6599\uff09\u3002\u7d14\u5438\u5149\u5ea6\u5de5\u4f5c\u96d9\u900f\u660e\u9762\u5c31\u884c\u3002<\/li>\n<li><strong>JGS2 \u6a19\u6e96<\/strong>\uff0c\u53ea\u6709\u61c9\u7528\u4e5f\u78b0\u5230 230 nm \u4ee5\u4e0b\u6df1\u7d2b\u5916\u6642\u624d\u7528 JGS1\u3002<\/li>\n<\/ul>\n\n<h3>\u5efa\u8b70\u6383\u63cf\u53c3\u6578<\/h3>\n<ul>\n<li>\u5347\u6eab\u901f\u7387\uff1a\u92b3\u5229\u7684 Tm \u5224\u5b9a\u7528 0.5\u20131.0 \u00b0C\/min\uff1b\u7be9\u9078\u7528\u6700\u9ad8 5 \u00b0C\/min\u3002<\/li>\n<li>\u6eab\u5ea6\u7bc4\u570d\uff1a25 \u5230 95 \u00b0C \u6db5\u84cb\u591a\u6578\u6c34\u6027 DNA \u5de5\u4f5c\uff1b\u5c0d\u66f4\u9577\u6216\u66f4\u7a69\u5b9a\u7684\u96d9\u87ba\u65cb\uff0c\u5ef6\u4f38\u5230 99 \u00b0C\u3002<\/li>\n<li>\u975e\u5bc6\u5c01\u914d\u7f6e\u6709\u6642\u7528\u7926\u7269\u6cb9\u8986\u84cb\u5c64\u7576\u984d\u5916\u7684\u84b8\u767c\u5c4f\u969c\uff1b\u5bc6\u5c01\u6bd4\u8272\u76bf\u8b93\u9019\u8b8a\u5f97\u6c92\u5fc5\u8981\u3002<\/li>\n<li>\u7de9\u885d\u6db2\uff1a10 mM Tris-HCl pH 7.5\u30011 mM EDTA\u3001100 mM NaCl \u662f\u6a19\u6e96\u3002\u5728\u5de5\u4f5c\u7bc4\u570d\u5167\u9e7d\u6fc3\u5ea6\u6bcf 10 mM NaCl \u4f7f Tm \u504f\u79fb\u7d04 1 \u00b0C\u3002<\/li>\n<\/ul><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg4-t\"><title id=\"svg4-t\">NADH \u5728 340 \u5948\u7c73\u5438\u5149\u5ea6\u7684\u6642\u7a0b\uff0c\u986f\u793a\u5178\u578b\u4e73\u9178\u812b\u6c2b\u9176\u9175\u7d20\u52d5\u529b\u5b78\u91cf\u6e2c\uff0c\u542b\u57fa\u8cea\u52a0\u5165\u3001\u521d\u901f\u8a08\u7b97\u8207 10 \u5206\u9418\u5167\u7684\u6307\u6578\u8870\u6e1b<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">340 nm \u7684 NADH \u52d5\u529b\u5b78\u6642\u7a0b \u2014 LDH \u53cd\u61c9<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">\u4e73\u9178\u812b\u6c2b\u9176\uff1aNADH + \u4e19\u916e\u9178 \u2192 NAD\u207a + \u4e73\u9178\uff1bA\u2083\u2084\u2080 \u96a8\u6642\u9593\u4e0b\u964d<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"240\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"120\" x2=\"680\" y2=\"120\"\/><line x1=\"80\" y1=\"180\" x2=\"680\" y2=\"180\"\/><line x1=\"80\" y1=\"240\" x2=\"680\" y2=\"240\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"300\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"300\"\/><\/g><line x1=\"80\" y1=\"300\" x2=\"680\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"316\" text-anchor=\"middle\">0<\/text><text x=\"200\" y=\"316\" text-anchor=\"middle\">2<\/text><text x=\"320\" y=\"316\" text-anchor=\"middle\">4<\/text><text x=\"440\" y=\"316\" text-anchor=\"middle\">6<\/text><text x=\"560\" y=\"316\" text-anchor=\"middle\">8<\/text><text x=\"680\" y=\"316\" text-anchor=\"middle\">10<\/text><\/g><text x=\"380\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u6642\u9593\uff08\u5206\u9418\uff09<\/text><text x=\"36\" y=\"180\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 180)\">A\u2083\u2084\u2080\uff08NADH \u5438\u5149\u5ea6\uff09<\/text><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"74\" y=\"64\" text-anchor=\"end\">1.0<\/text><text x=\"74\" y=\"124\" text-anchor=\"end\">0.7<\/text><text x=\"74\" y=\"184\" text-anchor=\"end\">0.4<\/text><text x=\"74\" y=\"244\" text-anchor=\"end\">0.2<\/text><text x=\"74\" y=\"304\" text-anchor=\"end\">0.05<\/text><\/g><path d=\"M 80 64 L 110 64 L 130 70\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><path d=\"M 130 70 L 200 130 L 280 200 L 360 240 L 440 265 L 520 280 L 600 290 L 680 295\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><line x1=\"130\" y1=\"60\" x2=\"130\" y2=\"300\" stroke=\"#16a34a\" stroke-width=\"1.5\" stroke-dasharray=\"4 3\"\/><text x=\"130\" y=\"56\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#16a34a\">\u52a0\u9175\u7d20<\/text><line x1=\"130\" y1=\"70\" x2=\"280\" y2=\"200\" stroke=\"#dc2626\" stroke-width=\"1.5\" stroke-dasharray=\"3 2\" opacity=\"0.7\"\/><text x=\"220\" y=\"120\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#dc2626\">\u521d\u901f v\u2080<\/text><text x=\"220\" y=\"134\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#dc2626\">\u524d 60 \u79d2\u7684\u659c\u7387<\/text><g transform=\"translate(440,80)\" font-family=\"Arial,sans-serif\"><rect x=\"0\" y=\"0\" width=\"220\" height=\"90\" fill=\"#fef9c3\" stroke=\"#ca8a04\" stroke-width=\"0.8\" rx=\"4\"\/><text x=\"10\" y=\"20\" font-size=\"11\" font-weight=\"700\" fill=\"#854d0e\">\u6bd4\u8272\u76bf\u9700\u6c42<\/text><text x=\"10\" y=\"38\" font-size=\"10\" fill=\"#444\">\u2022 10 mm \u5149\u7a0b\uff1b200\u2013500 \u00b5L<\/text><text x=\"10\" y=\"52\" font-size=\"10\" fill=\"#444\">\u2022 <5 \u5206\u9418 PTFE \u6469\u64e6\u84cb\u53ef\u4ee5<\/text><text x=\"10\" y=\"66\" font-size=\"10\" fill=\"#444\">\u2022 >5 \u5206\u9418\u7528\u585e\u6216\u87ba\u65cb\u84cb<\/text><text x=\"10\" y=\"80\" font-size=\"10\" fill=\"#444\">\u2022 25 \/ 37 \u00b0C \u4e0b Peltier \u00b10.1 \u00b0C \u6700\u7406\u60f3<\/text><\/g><\/svg><figcaption>\u5716 4 \u2014 \u5178\u578b\u7684 NADH \u52d5\u529b\u5b78\u6642\u7a0b\uff08LDH-\u4e19\u916e\u9178\uff09\u3002\u7de9\u885d\u6db2 + \u57fa\u8cea\u5728\u6bd4\u8272\u76bf\u88e1\u5e73\u8861\uff1b\u6253\u958b\u84cb\u5b50\u52a0\u9175\u7d20\uff1b\u84cb\u56de\u53bb\u4e26\u9023\u7e8c\u8a18\u9304\u5438\u5149\u5ea6\u3002\u524d\u7d04 60 \u79d2\u7684\u659c\u7387\uff08\u521d\u901f\uff09\u7d66\u51fa\u52d5\u529b\u5b78\u53c3\u6578\u3002\u5c0d\u8d85\u904e 5 \u5206\u9418\u7684\u904b\u884c\uff0c\u9700\u8981\u585e\u6216\u87ba\u65cb\u84cb\u4ee5\u9632\u84b8\u767c\u4f7f\u5f8c\u6bb5\u5c3e\u5df4\u504f\u5dee\u3002<\/figcaption><\/figure><h2 id=\"kinetics\">5. \u9175\u7d20\u52d5\u529b\u5b78 \u2014 \u7b49\u6eab\u6642\u7a0b\u91cf\u6e2c<\/h2>\n<p>\u9175\u7d20\u52d5\u529b\u5b78\u901a\u5e38\u7b49\u6eab\u904b\u884c\u5728 25 \u00b0C\uff08\u9ad4\u5916\u6a19\u6e96\uff09\u6216 37 \u00b0C\uff08\u751f\u7406\uff09\u3002\u6eab\u5ea6\u7dad\u6301\u6046\u5b9a\uff1b\u5be6\u9a57\u8a0a\u865f\u662f\u96a8\u57fa\u8cea\u6d88\u8017\u6216\u7522\u7269\u7d2f\u7a4d\u3001\u5438\u5149\u5ea6\u96a8\u6642\u9593\u7684\u8b8a\u5316\u3002\u52d5\u529b\u5b78\u904b\u884c\u5f9e 30 \u79d2\uff08\u5feb\u9175\u7d20\u3001\u505c\u6d41\uff09\u5230 60 \u5206\u9418\uff08\u6162\u9175\u7d20\u6216\u4f4e\u57fa\u8cea\u6fc3\u5ea6\uff09\uff1b\u904b\u884c\u8d8a\u9577\u5bc6\u5c01\u8d8a\u91cd\u8981\u3002<\/p>\n\n<h3>\u5e38\u898b\u6e2c\u5b9a\u8207\u6bd4\u8272\u76bf\u6db5\u7fa9<\/h3>\n<table>\n<thead><tr><th>\u6e2c\u5b9a\u985e\u5225<\/th><th>\u5178\u578b \u03bb<\/th><th>\u904b\u884c\u6642\u9577<\/th><th>\u6bd4\u8272\u76bf\u5efa\u8b70<\/th><\/tr><\/thead>\n<tbody>\n<tr><td>NAD \u4f9d\u8cf4\u7684\u812b\u6c2b\u9176\uff08LDH\u3001MDH\u3001ADH\u3001G6PDH\uff09<\/td><td>340 nm<\/td><td>1\u201310 \u5206\u9418<\/td><td>10 mm \u914d PTFE \u84cb\uff1b> 5 \u5206\u9418\u5247\u5bc6\u5c01<\/td><\/tr>\n<tr><td>\u904e\u6c27\u5316\u7269\u9176 \/ HRP \u5076\u806f\u6e2c\u5b9a\uff08TMB\u3001ABTS\uff09<\/td><td>650 \/ 405 nm<\/td><td>1\u20135 \u5206\u9418<\/td><td>10 mm \u914d PTFE \u84cb<\/td><\/tr>\n<tr><td>\u78f7\u9178\u9176\uff08\u5c0d\u785d\u57fa\u82ef\u78f7\u9178\uff09<\/td><td>405 nm<\/td><td>5\u201330 \u5206\u9418<\/td><td>10 mm \u52a0\u585e\u6216\u87ba\u65cb\u84cb<\/td><\/tr>\n<tr><td>\u86cb\u767d\u9176\uff08\u57fa\u8cea\u5c08\u4e00\uff09<\/td><td>\u8996\u60c5\u6cc1<\/td><td>10\u201360 \u5206\u9418<\/td><td>\u5efa\u8b70 10 mm \u87ba\u65cb\u84cb<\/td><\/tr>\n<tr><td>\u9577\u9175\u7d20\u6e2c\u5b9a\uff08\u6162\u5468\u8f49\u3001\u4f4e\u57fa\u8cea\uff09<\/td><td>\u8996\u60c5\u6cc1<\/td><td>> 60 \u5206\u9418<\/td><td>\u87ba\u65cb\u84cb\u5fc5\u5099<\/td><\/tr>\n<tr><td>\u7121\u7d30\u80de\u8f49\u9304\/\u8f49\u8b6f<\/td><td>\u8996\u60c5\u6cc1<\/td><td>1\u20134 \u5c0f\u6642<\/td><td>\u9644\u9694\u819c\u7684\u87ba\u65cb\u84cb\uff08\u4f9b\u53d6\u6a23\uff09<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<h3>\u5be6\u9a57\u4e2d\u52a0\u6a23\u7684\u554f\u984c<\/h3>\n<p>\u8a31\u591a\u9175\u7d20\u6e2c\u5b9a\u5148\u5728\u6bd4\u8272\u76bf\u88e1\u505a\u7de9\u885d\u6db2 + \u57fa\u8cea\u5e73\u8861\uff0c\u518d\u52a0\u9175\u7d20\u555f\u52d5\u53cd\u61c9\u3002\u52a0\u6a23\u6642\u5fc5\u9808\u53d6\u4e0b\u84cb\u5b50\u3001\u518d\u84cb\u56de\u3002\u4e09\u500b\u9078\u9805\uff1a<\/p>\n<ul>\n<li><strong>PTFE \u6469\u64e6\u84cb\uff1a<\/strong> \u6700\u5bb9\u6613\u53d6\u4e0b\u8207\u84cb\u56de\u3002\u9069\u5408\u91cd\u65b0\u84cb\u4e0a\u5f8c\u7684\u6eab\u5ea6\u5e73\u8861\u6642\u9593\u76f8\u5c0d\u904b\u884c\u5f88\u77ed\u7684\u77ed\u6e2c\u5b9a\u3002<\/li>\n<li><strong>PTFE \u585e\uff1a<\/strong> \u7cbe\u5bc6\u914d\u5408\u3001\u6bd4\u87ba\u65cb\u84cb\u66f4\u5feb\u84cb\u56de\uff1b\u6eab\u5ea6\u64fe\u52d5\u6700\u5c0f\u3002<\/li>\n<li><strong>\u9694\u819c\u84cb\uff08\u9644\u6a61\u81a0\u9694\u819c\u7684\u87ba\u65cb\u84cb\uff09\uff1a<\/strong> \u7528 Hamilton \u6ce8\u5c04\u5668\u7a7f\u904e\u9694\u819c\u6ce8\u5165\uff1b\u84cb\u5b50\u5f9e\u4e0d\u53d6\u4e0b\u3002\u6700\u9069\u5408\u5bc6\u5c01\u6e2c\u5b9a\uff08\u53ad\u6c27\u3001\u63ee\u767c\uff09\uff0c\u4f46\u9700\u8981\u6280\u5de7\u3002<\/li>\n<\/ul>\n\n<div class=\"csg-callout\"><strong>\u5c0d\u4f4e NADH \u6fc3\u5ea6\u7684 NADH \u6e2c\u5b9a\uff08340 nm\uff09\uff0c<\/strong> \u5149\u8b5c\u5100\u6bd4\u8272\u76bf\u5ea7\u7684\u6eab\u5ea6\u7a69\u5b9a\u6027\u6bd4\u6bd4\u8272\u76bf\u5bc6\u5c01\u66f4\u8981\u7dca\uff0c\u56e0\u70ba NADH \u5438\u5149\u5ea6\u5f88\u5c0f\uff085 \u00b5M \u6642 A < 0.05 AU\uff09\uff0c\u800c\u6bcf 10 \u00b0C 1% \u7684\u6f02\u79fb\u8207\u52d5\u529b\u5b78\u8a0a\u865f\u76f8\u7576\u3002\u7528\u6709\u8a18\u9304 \u00b10.1 \u00b0C \u7a69\u5b9a\u6027\u7684 Peltier \u63a7\u6eab\u5ea7\u3002<\/div><h2 id=\"stoppedflow\">6. \u505c\u6d41\u52d5\u529b\u5b78 \u2014 \u6d41\u901a\u6c60\u7684\u60c5\u6cc1<\/h2>\n<p>\u505c\u6d41\u5149\u8b5c\u4ee5\u6a5f\u68b0\u65b9\u5f0f\u5728\u4e00\u500b\u5c0f\u6df7\u5408\u5ba4\u4e2d\u5408\u4f75\u5169\u80a1\u8a66\u5291\u6d41\u3001\u7136\u5f8c\u300c\u505c\u6b62\u300d\u6d41\u52d5\u4e26\u5728\u6df7\u5408\u5668\u4e0b\u6e38\u7684\u89c0\u6e2c\u6bd4\u8272\u76bf\u4e2d\u8a18\u9304\u5438\u5149\u5ea6\u6642\u7a0b\uff0c\u4f86\u91cf\u6e2c\u975e\u5e38\u5feb\u7684\u53cd\u61c9\uff08\u6beb\u79d2\u5230\u79d2\u7684\u6642\u9593\u5c3a\u5ea6\uff09\u3002\u9019\u88e1\u7684\u6bd4\u8272\u76bf\u662f\u4e00\u500b\u5e36\u9032\u51fa\u53e3\u57e0\u3001\u88dd\u5728\u63a7\u6eab\u6bd4\u8272\u76bf\u5ea7\u88e1\u7684\u6d41\u901a\u6c60\u3002<\/p>\n\n<h3>\u505c\u6d41\u5c0d\u6bd4\u8272\u76bf\u7684\u9700\u6c42<\/h3>\n<ul>\n<li><strong>\u9032\u53e3\u8207\u51fa\u53e3\u57e0<\/strong> \uff08\u901a\u5e38 4 mm \u5916\u5f91\u73bb\u7483\u5c0d\u73bb\u7483\u7ba1\u8def\u9023\u63a5\uff09\uff0c\u8b93\u6ce8\u5c04\u5668\u80fd\u628a\u8a66\u5291\u63a8\u904e\u6bd4\u8272\u76bf\u3002<\/li>\n<li><strong>\u77ed\u5149\u7a0b<\/strong> \u2014\u2014\u901a\u5e38 1\u30012 \u6216 3 mm\u2014\u2014\u4ee5\u628a\u6df7\u5408\u5668\u8207\u89c0\u6e2c\u9ede\u4e4b\u9593\u7684\u6b7b\u6642\u9593\u964d\u5230\u6700\u4f4e\u3002\u6a19\u6e96 10 mm \u5149\u7a0b\u6709\u6642\u7528\u65bc\u6162\u53cd\u61c9\uff0c\u4f46\u6b7b\u6642\u9593\u7684\u4ee3\u50f9\u662f\u771f\u5be6\u7684\u3002<\/li>\n<li><strong>\u56db\u900f\u660e\u9762\u6216\u96d9\u900f\u660e\u9762<\/strong> \u8996\u4f60\u662f\u5426\u4e5f\u6536\u96c6\u87a2\u5149\uff08\u5167\u6e90\u87a2\u5149\u3001\u7d50\u5408\u67d3\u6599\u3001FRET\uff09\u800c\u5b9a\u3002<\/li>\n<li><strong>200\u2013700 \u00b5L \u9ad4\u7a4d<\/strong> \u662f\u89c0\u6e2c\u5ba4\u7684\u5178\u578b\uff1b\u8f03\u5c0f\u9ad4\u7a4d\u7d66\u66f4\u5feb\u7684\u6df7\u5408\u3001\u4f46\u5149\u5b78\u5c0d\u6e96\u66f4\u96e3\u3002<\/li>\n<li><strong>\u6a19\u6e96 12.5 \u00d7 12.5 mm \u5916\u90e8\u5c3a\u5bf8<\/strong> \u4ee5\u76f8\u5bb9\u65bc\u88fd\u9020\u5546\u7684\u5b89\u88dd\u5f0f\u6bd4\u8272\u76bf\u584a\u3002<\/li>\n<\/ul>\n\n<p>\u95dc\u65bc\u6d41\u901a\u6c60\u5e7e\u4f55\uff08\u65b9\u5f62 vs \u5713\u67f1\u5f62\u3001\u6b7b\u9ad4\u7a4d\u3001\u6df7\u5408\u6548\u7387\uff09\u7684\u66f4\u6df1\u5165\u8655\u7406\uff0c\u898b\u6211\u5011\u5728 <a href=\"https:\/\/machinedquartz.com\/cuvettes-and-cells-size-chart\/\">\u6bd4\u8272\u76bf\u5c3a\u5bf8\u8868<\/a>.<\/p><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 340\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg5-t\"><title id=\"svg5-t\">\u71b1\u97ff\u61c9\u6642\u9593\u6bd4\u8f03\uff0c\u986f\u793a\u6a23\u54c1\u88dd\u5165\u4e0d\u540c\u6eab\u5ea6\u7684 Peltier \u5ea7\u6642\u6bd4\u8272\u76bf\u58c1\u539a\u5982\u4f55\u5f71\u97ff\u5e73\u8861\u6642\u9593\uff0c\u8584\u58c1\u77f3\u82f1\u6bd4\u539a\u58c1\u73bb\u7483\u66f4\u5feb\u9054\u5e73\u8861<\/title><rect width=\"720\" height=\"340\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u71b1\u97ff\u61c9 \u2014 \u6a23\u54c1\u591a\u5feb\u9054\u5230\u5ea7\u7684\u6eab\u5ea6\uff1f<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">25 \u00b0C \u7684\u6a23\u54c1\u88dd\u5165 65 \u00b0C \u7684 Peltier \u5ea7\uff1b\u5e73\u8861\u5230\u76ee\u6a19\u7684 \u00b10.5 \u00b0C<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"220\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"120\" x2=\"680\" y2=\"120\"\/><line x1=\"80\" y1=\"180\" x2=\"680\" y2=\"180\"\/><line x1=\"80\" y1=\"240\" x2=\"680\" y2=\"240\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"280\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"280\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"280\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"280\"\/><\/g><line x1=\"80\" y1=\"280\" x2=\"680\" y2=\"280\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"280\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"296\" text-anchor=\"middle\">0<\/text><text x=\"200\" y=\"296\" text-anchor=\"middle\">60<\/text><text x=\"320\" y=\"296\" text-anchor=\"middle\">120<\/text><text x=\"440\" y=\"296\" text-anchor=\"middle\">180<\/text><text x=\"560\" y=\"296\" text-anchor=\"middle\">240<\/text><text x=\"680\" y=\"296\" text-anchor=\"middle\">300<\/text><\/g><text x=\"380\" y=\"314\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u88dd\u5165\u5f8c\u7684\u6642\u9593\uff08\u79d2\uff09<\/text><text x=\"36\" y=\"170\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 170)\">\u6a23\u54c1\u6eab\u5ea6\uff08\u00b0C\uff09<\/text><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"74\" y=\"64\" text-anchor=\"end\">65<\/text><text x=\"74\" y=\"124\" text-anchor=\"end\">55<\/text><text x=\"74\" y=\"184\" text-anchor=\"end\">45<\/text><text x=\"74\" y=\"244\" text-anchor=\"end\">35<\/text><text x=\"74\" y=\"280\" text-anchor=\"end\">25<\/text><\/g><line x1=\"80\" y1=\"64\" x2=\"680\" y2=\"64\" stroke=\"#16a34a\" stroke-width=\"1.5\" stroke-dasharray=\"6 3\" opacity=\"0.6\"\/><text x=\"690\" y=\"68\" text-anchor=\"end\" font-family=\"Arial,sans-serif\" font-size=\"10\" font-weight=\"700\" fill=\"#16a34a\">\u76ee\u6a19 65 \u00b0C<\/text><path d=\"M 80 280 Q 110 200 145 130 Q 165 100 200 80 Q 240 70 280 67 L 680 64\" stroke=\"#0ea5e9\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"200\" y=\"96\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#0369a1\">1 mm \u8584\u58c1\u77f3\u82f1<\/text><text x=\"200\" y=\"110\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#0369a1\">~30 \u79d2\u5230 \u00b10.5 \u00b0C<\/text><path d=\"M 80 280 Q 130 240 180 200 Q 230 168 280 142 Q 340 110 400 84 L 500 70 L 680 64\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"370\" y=\"156\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#1a2a6c\">1.5 mm \u6a19\u6e96\u77f3\u82f1<\/text><text x=\"370\" y=\"170\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#1a2a6c\">~90 \u79d2\u5230 \u00b10.5 \u00b0C<\/text><path d=\"M 80 280 Q 150 250 220 230 Q 320 210 420 180 Q 500 150 580 110 L 680 80\" stroke=\"#dc2626\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"500\" y=\"200\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#dc2626\">3 mm \u539a\u58c1\u73bb\u7483<\/text><text x=\"500\" y=\"214\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#dc2626\">~240 \u79d2\u5230 \u00b10.5 \u00b0C<\/text><\/svg><figcaption>\u5716 5 \u2014 \u6a23\u54c1\u88dd\u5165\u71b1\u5ea7\u5f8c\u5728\u6bd4\u8272\u76bf\u5167\u7684\u71b1\u97ff\u61c9\u3002\u8584\u58c1\u77f3\u82f1\u6bd4\u8272\u76bf\u7d04 30 \u79d2\u5e73\u8861\uff1b\u6a19\u6e96 1.5 mm \u58c1\u539a\u7d04 90 \u79d2\uff1b\u539a\u58c1\u5149\u5b78\u73bb\u7483\u6bd4\u8272\u76bf\uff083 mm \u58c1\uff09\u7d04 4 \u5206\u9418\u3002\u5c0d 0.5 \u00b0C\/min \u7684 DNA \u7194\u89e3\u8207 < 5 \u5206\u9418\u7684\u9175\u7d20\u52d5\u529b\u5b78\u904b\u884c\uff0c\u5e73\u8861\u6642\u9593\u5f88\u8981\u7dca\u2014\u2014\u9078\u8584\u58c1\u7194\u878d\u77f3\u82f1\u3001\u5225\u9078\u539a\u58c1\u73bb\u7483\u3002<\/figcaption><\/figure><h2 id=\"thermal\">7. \u71b1\u97ff\u61c9\u6642\u9593\u8207\u5747\u52fb\u5ea6<\/h2>\n<p>\u5169\u500b\u7269\u7406\u6027\u8cea\u6c7a\u5b9a\u6bd4\u8272\u76bf\u5167\u6a23\u54c1\u591a\u5feb\u9054\u5230\u5ea7\u7684\u6eab\u5ea6\uff1a\u6bd4\u8272\u76bf\u58c1\u539a\u8207\u6750\u6599\u5c0e\u71b1\u4fc2\u6578\u3002\u7194\u878d\u77f3\u82f1\u5c0e\u71b1\u4fc2\u6578 1.4 W\/m\u00b7K\uff0c\u5149\u5b78\u73bb\u7483\uff08BK7 \u7d1a\uff09\u7d04 1.1\uff0cPMMA \u53ea\u6709 0.19\uff08PMMA \u6bd4\u8272\u76bf\u4e0d\u9069\u5408\u5feb\u901f\u6eab\u5ea6\u5de5\u4f5c\uff09\u3002\u58c1\u539a\u4e0d\u4e00\uff1a\u8584\u58c1\u77f3\u82f1\uff081 mm \u58c1\uff09\u6bd4\u6a19\u6e96\uff081.5 mm\uff09\u3001\u6bd4\u539a\u58c1\u73bb\u7483\uff083 mm\uff09\u66f4\u5feb\u5e73\u8861\u3002<\/p>\n\n<h3>\u9019\u5c0d\u4f60\u5be6\u9a57\u7684\u610f\u7fa9<\/h3>\n<ul>\n<li><strong>0.5\u20131 \u00b0C\/min \u7684 DNA \u7194\u89e3\uff1a<\/strong> \u6a23\u54c1\u9700\u5728 0.1 \u00b0C \u5167\u8ddf\u4e0a\u5ea7\u7684\u6eab\u5ea6\u3002\u8584\u58c1\u77f3\u82f1\uff1b\u5e73\u8861\u6642\u9593\u6bd4\u6383\u63cf\u6b65\u77ed\u3002<\/li>\n<li><strong>\u9175\u7d20\u52d5\u529b\u5b78\u904b\u884c\uff1a<\/strong> \u88dd\u5165\u7de9\u885d\u6db2 + \u57fa\u8cea\u5f8c\uff0c\u52a0\u9175\u7d20\u524d\u7559 60\u2013120 \u79d2\u505a\u71b1\u5e73\u8861\u3002\u9019\u7a97\u53e3\u5167\u7684\u8b80\u51fa\u4e0d\u7a69\u5b9a\u3002<\/li>\n<li><strong>\u505c\u6d41\uff1a<\/strong> \u5c0f\u7684\u6d41\u901a\u6c60\u9ad4\u7a4d\u5e7e\u79d2\u5c31\u5e73\u8861\u5230\u5ea7\u7684\u6eab\u5ea6\uff1b\u6c7a\u5b9a\u6027\u7684\u9650\u5236\u662f\u6ce8\u5c04\u5668\u5167\u5bb9\u7269\u8207\u6df7\u5408\u5ba4\u58c1\u90fd\u8981\u9810\u5148\u5e73\u8861\uff0c\u800c\u4e0d\u662f\u5149\u5b78\u6bd4\u8272\u76bf\u672c\u8eab\u3002<\/li>\n<\/ul>\n\n<h3>\u6bd4\u8272\u76bf\u5167\u7684\u6eab\u5ea6\u5747\u52fb\u5ea6<\/h3>\n<p>Peltier \u5ea7\u88e1\u7684\u6bd4\u8272\u76bf\u900f\u904e\u91d1\u5c6c\u5c0d\u77f3\u82f1\u7684\u63a5\u89f8\u5f9e\u5e95\u90e8\u8207\u5074\u9762\u52a0\u71b1\/\u51b7\u537b\u3001\u4e26\u900f\u904e\u958b\u653e\u6216\u52a0\u84cb\u7684\u7a7a\u6c23\u9593\u9699\u5f9e\u9802\u90e8\u52a0\u71b1\/\u51b7\u537b\u3002\u6a23\u54c1\u5167\u90e8\u767c\u5c55\u51fa\u4e00\u500b\u5c0f\u7684\u5782\u76f4\u71b1\u68af\u5ea6\uff08~0.1\u20130.3 \u00b0C\uff09\uff0c\u52a0\u71b1\u6642\u5e95\u90e8\u8f03\u6696\u3001\u51b7\u537b\u6642\u9802\u90e8\u8f03\u6696\u3002\u9019\u68af\u5ea6\u5728\u5438\u5149\u5ea6\u88e1\u770b\u4e0d\u898b\uff08\u5149\u675f\u7a7f\u904e\u4e2d\u5fc3\uff09\uff0c\u4f46\u5c0d \u00b10.1 \u00b0C \u6821\u6b63\u5f88\u95dc\u9375\u7684\u975e\u5e38\u7cbe\u78ba\u7194\u89e3\u66f2\u7dda\u5de5\u4f5c\u5f88\u8981\u7dca\u3002\u652a\u62cc\u6bd4\u8272\u76bf\uff08\u7528\u78c1\u652a\u62cc\u5b50\u6216\u8072\u6ce2\u652a\u52d5\uff09\u6d88\u9664\u68af\u5ea6\u3001\u4ee3\u50f9\u662f\u6a5f\u68b0\u8907\u96dc\u5ea6\u3002<\/p>\n\n<div class=\"csg-callout warn\"><strong>\u6eab\u5ea6\u5de5\u4f5c 60 \u00b0C \u4ee5\u4e0a\u4e0d\u8981\u7528\u5851\u81a0\u6bd4\u8272\u76bf\u3002<\/strong> PMMA \u5728 80\u2013100 \u00b0C \u8edf\u5316\u3001\u5230 70 \u00b0C \u5c31\u986f\u51fa\u770b\u5f97\u898b\u7684\u8b8a\u5f62\uff1b\u805a\u82ef\u4e59\u70ef\u5230 90 \u00b0C\u3002\u5c3a\u5bf8\u8b8a\u5316\u4f7f\u5149\u7a0b\u504f\u5dee\u3002\u805a\u78b3\u9178\u916f\u8f03\u5805\u56fa\uff08Tg ~140 \u00b0C\uff09\uff0c\u4f46\u5c0d 70 \u00b0C \u4ee5\u4e0a\u7684\u7194\u89e3\u66f2\u7dda\u4ecd\u4e0d\u662f\u5c0d\u7684\u9078\u64c7\u3002\u7528\u7194\u878d\u77f3\u82f1\u3002<\/div><h2 id=\"decision\">8. \u6c7a\u7b56\u77e9\u9663 \u2014 \u5be6\u9a57\u5c0d\u6bd4\u8272\u76bf\u985e\u5225<\/h2>\n<p>\u4e0b\u9762\u7684\u77e9\u9663\u628a\u516b\u7a2e\u5e38\u898b\u7684\u63a7\u6eab UV-Vis \u5be6\u9a57\u985e\u578b\u5c0d\u61c9\u5230 MachinedQuartz \u7522\u54c1\u985e\u5225\u3002\u7576\u4f5c\u8d77\u9ede\u7528\uff1b\u5177\u9ad4\u7684\u5149\u7a0b\u3001\u9ad4\u7a4d\u8207\u7b49\u7d1a\u53d6\u6c7a\u65bc\u4f60\u7684\u6a23\u54c1\u3002<\/p>\n<table class=\"csg-decision\">\n<thead><tr><th>\u5be6\u9a57<\/th><th>\u5efa\u8b70\u5bc6\u5c01<\/th><th>\u5149\u7a0b<\/th><th>\u5e7e\u4f55<\/th><th>MQ \u985e\u5225<\/th><\/tr><\/thead>\n<tbody>\n<tr><td><strong>DNA \u7194\u89e3\uff0825\u219295 \u00b0C\u300130\u201390 \u5206\u9418\uff09<\/strong><\/td><td class=\"rec\">\u87ba\u65cb\u84cb\uff08\u5fc5\u5099\uff09<\/td><td>10 mm<\/td><td>\u96d9\u9762\u6216\u56db\u9762<\/td><td>\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf<\/td><\/tr>\n<tr><td><strong>\u86cb\u767d\u8cea\u71b1\u7a69\u5b9a\u6027\uff08CD \u6216 A280\uff09<\/strong><\/td><td class=\"rec\">\u87ba\u65cb\u84cb\u6216\u7cbe\u5bc6\u585e<\/td><td>1\u201310 mm<\/td><td>\u504f\u597d\u56db\u9762<\/td><td>\u87ba\u65cb\u84cb\u6216\u52a0\u585e<\/td><\/tr>\n<tr><td><strong>NAD \u4f9d\u8cf4\u52d5\u529b\u5b78\uff0c<5 \u5206\u9418<\/strong><\/td><td class=\"ok\">PTFE \u6469\u64e6\u84cb\u53ef\u63a5\u53d7<\/td><td>10 mm<\/td><td>\u96d9\u9762<\/td><td>\u6a19\u6e96\u6bd4\u8272\u76bf<\/td><\/tr>\n<tr><td><strong>\u6162\u9175\u7d20\u52d5\u529b\u5b78\uff0c5\u201360 \u5206\u9418<\/strong><\/td><td class=\"rec\">\u52a0\u585e\u6216\u87ba\u65cb\u84cb<\/td><td>10 mm<\/td><td>\u96d9\u9762<\/td><td>\u52a0\u585e\u6bd4\u8272\u76bf<\/td><\/tr>\n<tr><td><strong>\u9577\u9175\u7d20\u52d5\u529b\u5b78\uff0c>60 \u5206\u9418<\/strong><\/td><td class=\"rec\">\u9644\u9694\u819c\u7684\u87ba\u65cb\u84cb<\/td><td>10 mm<\/td><td>\u96d9\u9762<\/td><td>\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf<\/td><\/tr>\n<tr><td><strong>\u53ad\u6c27 \/ \u5c0d\u6c27\u654f\u611f\u7684\u52d5\u529b\u5b78<\/strong><\/td><td class=\"rec\">\u9644\u9694\u819c\u7684\u87ba\u65cb\u84cb<\/td><td>10 mm<\/td><td>\u96d9\u9762<\/td><td>\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf<\/td><\/tr>\n<tr><td><strong>\u505c\u6d41\u52d5\u529b\u5b78\uff08\u6beb\u79d2\uff09<\/strong><\/td><td class=\"rec\">\u5e36\u57e0\u7684\u6d41\u901a\u6c60<\/td><td>1\u20133 mm<\/td><td>\u56db\u9762<\/td><td>\u6d41\u901a\u6c60<\/td><\/tr>\n<tr><td><strong>\u9023\u7e8c\u6d41\u88fd\u7a0b\u76e3\u6e2c<\/strong><\/td><td class=\"rec\">\u5e36\u57e0\u7684\u6d41\u901a\u6c60<\/td><td>5\u201310 mm<\/td><td>\u96d9\u9762<\/td><td>\u6d41\u901a\u6c60<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<p>\u5c0d DNA \u7194\u89e3\u8207\u4efb\u4f55 60 \u00b0C \u4ee5\u4e0a\u7684\u5de5\u4f5c\uff0c\u9644 PTFE \u5167\u896f\u7684\u87ba\u65cb\u84cb\u6c92\u5f97\u5546\u91cf\u3002\u5c0d\u5ba4\u6eab\u4e0b 5 \u5206\u9418\u4ee5\u5167\u7684\u52d5\u529b\u5b78\uff0c\u6a19\u6e96 PTFE \u6469\u64e6\u84cb\u5c31\u884c\u3002\u4e2d\u9593\u7bc4\u570d\u2014\u201425 \u6216 37 \u00b0C \u4e0b 5 \u5230 60 \u5206\u9418\u7684\u52d5\u529b\u5b78\u904b\u884c\u2014\u2014\u662f\u5bc6\u5c01\u5c64\u7d1a\u9078\u64c7\u5c0d\u6578\u64da\u54c1\u8cea\u5f71\u97ff\u6700\u5927\u7684\u5730\u65b9\u3002<\/p><h2 id=\"skus\">9. MachinedQuartz \u578b\u9304\u5165\u53e3<\/h2>\n<p>\u4e09\u500b\u7522\u54c1\u985e\u5225\u57fa\u672c\u4e0a\u6db5\u84cb\u6240\u6709\u63a7\u6eab\u6bd4\u8272\u76bf\u5de5\u4f5c\u3002\u6bcf\u4e00\u500b\u90fd\u662f\u6a6b\u8de8\u5149\u7a0b\u3001\u9ad4\u7a4d\u8207\u5e7e\u4f55\u7684\u591a SKU \u5099\u8ca8\u7cfb\u5217\u3002<\/p>\n\n<div class=\"csg-related-grid\">\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-screw-top-cuvettes\/\"><span class=\"csg-related-icon\">\ud83d\udd12<\/span><span class=\"csg-related-h\">\u77f3\u82f1\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf<\/span><span class=\"csg-related-d\">119 \u500b SKU \u2014 \u5bc6\u5c01\u52d5\u529b\u5b78\u904b\u884c\u3001DNA \u7194\u89e3\u3001\u53ad\u6c27\u5de5\u4f5c\u7684\u4e3b\u529b\u3002\u5149\u7a0b 0.5\u2013100 mm\u3001\u9ad4\u7a4d 175 \u00b5L\u2013125 mL\u3001\u96d9\u9762\u8207\u56db\u9762\u900f\u5149\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-stoppered-cuvettes\/\"><span class=\"csg-related-icon\">\ud83d\udca3<\/span><span class=\"csg-related-h\">\u77f3\u82f1\u52a0\u585e\u6bd4\u8272\u76bf<\/span><span class=\"csg-related-d\">36 \u500b SKU \u2014 \u7cbe\u5bc6\u914d\u5408\u7684 PTFE \u585e\uff1b\u7d66\u6709\u5be6\u9a57\u4e2d\u52a0\u6a23\u30015\u201360 \u5206\u9418\u904b\u884c\u7684\u52d5\u529b\u5b78\u5de5\u4f5c\u3002\u6bd4\u87ba\u65cb\u84cb\u66f4\u5feb\u53d6\u51fa\/\u63d2\u5165\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/product-category\/products\/cuvettes\/quartz-flow-cells\/\"><span class=\"csg-related-icon\">\ud83d\udeab<\/span><span class=\"csg-related-h\">\u77f3\u82f1\u6d41\u901a\u6c60<\/span><span class=\"csg-related-d\">78 \u500b SKU \u2014 \u5e36\u9032\/\u51fa\u53e3\u57e0\u7684\u6d41\u901a\u6bd4\u8272\u76bf\uff0c\u4f9b\u505c\u6d41\u3001\u9023\u7e8c\u6d41\u88fd\u7a0b\u76e3\u6e2c\u3001\u7dda\u4e0a HPLC \u5075\u6e2c\u3002\u5149\u7a0b 0.5\u201310 mm\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/screw-top-cuvette-guide\/\"><span class=\"csg-related-icon\">\ud83d\udcd6<\/span><span class=\"csg-related-h\">\u87ba\u65cb\u84cb\u6df1\u5165<\/span><span class=\"csg-related-d\">\u84cb\u5b50\u8207\u5167\u896f\u6750\u6599\u9078\u64c7\uff08PTFE \/ \u77fd\u81a0 \/ Viton \/ \u4e01\u57fa \/ EPDM\uff09\u300124 \u5c0f\u6642\u5bc6\u5c01\u8868\u73fe\u3001\u6bd4\u8272\u76bf\u672c\u9ad4\u53d6\u6368\u3002<\/span><\/a>\n<\/div>\n\n<div class=\"csg-cta-box\"><h3>\u9700\u8981\u6046\u6eab\u6bd4\u8272\u76bf\u5831\u50f9\uff1f<\/h3><p>\u628a\u5be6\u9a57\u985e\u578b\uff08DNA \u7194\u89e3 \/ \u52d5\u529b\u5b78 \/ \u505c\u6d41\uff09\u3001\u5149\u8b5c\u5100\u578b\u865f\u8207\u6578\u91cf\u5bc4\u4f86\u3002\u6211\u5011\u5728\u4e00\u500b\u5de5\u4f5c\u5929\u5167\u4ee5\u5c0d\u7684 SKU \u8207 CoA \u6587\u4ef6\u56de\u8986\u3002<\/p><a href=\"https:\/\/machinedquartz.com\/contact\/\" class=\"csg-cta-btn\">\u7d22\u53d6\u5831\u50f9 \u2192<\/a><a href=\"https:\/\/machinedquartz.com\/bulk-quartz-cuvettes\/\" class=\"csg-cta-btn outline\">\u5927\u5b97\u8207 OEM \u2192<\/a><\/div><h2 id=\"tools\">10. \u76f8\u95dc\u6307\u5357<\/h2>\n<div class=\"csg-related-grid\">\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/screw-top-cuvette-guide\/\"><span class=\"csg-related-icon\">\ud83d\udd12<\/span><span class=\"csg-related-h\">\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u6307\u5357<\/span><span class=\"csg-related-d\">\u9589\u5408\u578b\u5f0f\u3001\u84cb\u5b50\u5167\u896f\u6750\u6599\u3001\u5bc6\u5c01\u8868\u73fe \u2014 \u6df1\u5165\u6280\u8853\u6307\u5357\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/cuvette-path-length-by-analyte\/\"><span class=\"csg-related-icon\">\ud83d\udcd0<\/span><span class=\"csg-related-h\">\u4f9d\u5f85\u6e2c\u7269\u5206\u7684\u5149\u7a0b<\/span><span class=\"csg-related-d\">\u70ba\u86cb\u767d\u8cea\u3001DNA\u3001\u9175\u7d20\u8f14\u56e0\u5b50\u8207\u53e6\u5916 5 \u7a2e\u5f85\u6e2c\u7269\u985e\u5225\u6311\u5c0d\u5149\u7a0b\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/uv-vis-troubleshooting-guide\/\"><span class=\"csg-related-icon\">\ud83d\udd27<\/span><span class=\"csg-related-h\">UV-Vis \u7591\u96e3\u6392\u89e3<\/span><span class=\"csg-related-d\">\u8a3a\u65b7\u6c23\u6ce1\u3001\u689d\u7d0b\u3001\u57fa\u7dda\u6f02\u79fb \u2014 \u63a7\u6eab\u51fa\u932f\u6642\u6703\u58de\u4ec0\u9ebc\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/cuvette-spectrophotometer-verification-usp-ep\/\"><span class=\"csg-related-icon\">\ud83d\udccb<\/span><span class=\"csg-related-h\">USP <851> \u9a57\u8b49<\/span><span class=\"csg-related-d\">\u53d7\u898f\u7bc4\u52d5\u529b\u5b78\u5de5\u4f5c\u7684\u6ce2\u9577 + \u5438\u5149\u5ea6 + \u5149\u7a0b\u9a57\u8b49\u898f\u7a0b\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/cuvette-cleaning-protocol\/\"><span class=\"csg-related-icon\">\ud83e\uddec<\/span><span class=\"csg-related-h\">\u6e05\u6d17\u898f\u7a0b<\/span><span class=\"csg-related-d\">\u5728\u6a23\u54c1\u7d44\u4e4b\u9593\u8b93\u5bc6\u5c01\u6bd4\u8272\u76bf\u7121\u6c61\u67d3\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/bulk-quartz-cuvettes\/\"><span class=\"csg-related-icon\">\ud83d\udce6<\/span><span class=\"csg-related-h\">\u5927\u5b97\u8207 OEM<\/span><span class=\"csg-related-d\">\u91cf\u8a02\u3001\u5ba2\u88fd\u5e7e\u4f55\u3001FedEx DAP\u30012 \u4ef6\u8d77\u8a02\u3001\u5b8c\u6574\u54c1\u7ba1\u6587\u4ef6\u3002<\/span><\/a>\n<\/div><h2 id=\"faq\">11. \u5e38\u898b\u554f\u984c<\/h2>\n<div class=\"csg-faq\">\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">DNA \u7194\u89e3\u66f2\u7dda\u9700\u8981\u7279\u6b8a\u7684\u6bd4\u8272\u76bf\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u662f\uff0c\u610f\u601d\u662f\u6bd4\u8272\u76bf\u5728\u6574\u500b\u6eab\u5ea6\u6383\u63cf\u671f\u9593\u5fc5\u9808\u5bc6\u5c01\u3002DNA \u5f9e 25 \u5230 95 \u00b0C \u7194\u89e3\u901a\u5e38\u8981 30 \u5230 90 \u5206\u9418\uff1b\u958b\u653e\u6216 PTFE \u6469\u64e6\u84cb\u6bd4\u8272\u76bf\u5728\u904b\u884c\u4e2d\u84b8\u767c\u640d\u5931 3 \u5230 8% \u7684\u9ad4\u7a4d\uff0c\u6fc3\u7e2e DNA \u4e26\u4f7f\u8868\u89c0 A260 \u57fa\u7dda\u504f\u5dee\u3002\u9644 PTFE \u5167\u896f\u7684\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u662f\u6a19\u6e96\u9078\u64c7\u3002\u5916\u90e8\u5c3a\u5bf8\u3001\u5149\u5b78\u5e7e\u4f55\uff08\u96d9\u9762\u6216\u56db\u9762\u900f\u5149\uff09\u8207\u5149\u7a0b\uff08\u5178\u578b 10 mm\uff09\u5247\u8207\u4efb\u4f55\u5176\u4ed6 UV-Vis \u6bd4\u8272\u76bf\u76f8\u540c\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u80fd\u5728 Peltier \u63a7\u6eab\u6bd4\u8272\u76bf\u5ea7\u88e1\u7528\u6a19\u6e96 10 mm \u6bd4\u8272\u76bf\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u53ef\u4ee5\u3002Peltier \u5ea7\u8a2d\u8a08\u4f86\u63a5\u53d7\u901a\u7528\u7684 12.5 \u00d7 12.5 \u00d7 45 mm \u5916\u90e8\u5c3a\u5bf8\u6bd4\u8272\u76bf\u3002Peltier \u5143\u4ef6\u900f\u904e\u5ea7\u7684\u91d1\u5c6c\u58c1\u8207\u6bd4\u8272\u76bf\u672c\u9ad4\u505a\u71b1\u63a5\u89f8\u3002\u4e00\u652f\u6a19\u6e96 MachinedQuartz 10 mm \u6bd4\u8272\u76bf\u5728\u4efb\u4f55\u73fe\u4ee3 Peltier \u5ea7\u88e1\u90fd\u88dd\u5f97\u9032\u3001\u4e5f\u80fd\u7528\u3002\u9650\u5236\u662f\u6bd4\u8272\u76bf\u5bc6\u5c01\u3001\u4e0d\u662f\u5e7e\u4f55\uff1a\u5c0d\u5728\u8207\u74b0\u5883\u4e0d\u540c\u6eab\u5ea6\u4e0b\u8d85\u904e\u5e7e\u5206\u9418\u7684\u904b\u884c\uff0c\u7528\u87ba\u65cb\u84cb\u6216\u52a0\u585e\u6bd4\u8272\u76bf\u4ee5\u9632\u84b8\u767c\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u52a0\u585e\u6bd4\u8272\u76bf\u8207\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u5dee\u5728\u54ea\uff1f<\/div><div class=\"csg-faq-a\"><p>\u52a0\u585e\u6bd4\u8272\u76bf\u6709\u4e00\u500b\u914d\u5728\u6bd4\u8272\u76bf\u672c\u9ad4\u78e8\u7802\u73bb\u7483\u627f\u5ea7\u4e0a\u7684 PTFE \u6216\u73bb\u7483\u585e\u2014\u2014\u6469\u64e6\u914d\u5408\u3001\u5feb\u901f\u63d2\u5165\u8207\u53d6\u51fa\u300130 \u5206\u9418\u5167\u5bc6\u5c01\u8868\u73fe\u7d04 95%\u3002\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u6709\u4e00\u500b\u9644 PTFE \u5167\u896f\u7684\u87ba\u7d0b\u84cb\u2014\u2014\u56e0\u70ba\u8981\u9396\u87ba\u7d0b\u6240\u4ee5\u63d2\u5165\u8f03\u6162\u3001\u591a\u5c0f\u6642\u904b\u884c\u5bc6\u5c01\u8868\u73fe\u8d85\u904e 99%\uff0c\u5c0d DNA \u7194\u89e3\u3001\u53ad\u6c27\u5de5\u4f5c\u8207\u4efb\u4f55\u63ee\u767c\u6eb6\u5291\u61c9\u7528\u90fd\u5fc5\u5099\u3002\u9700\u8981\u5728\u5be6\u9a57\u4e2d\u52a0\u6a23\u6642\u7528\u52a0\u585e\uff1b\u9700\u8981\u9577\u6642\u9593\u3001\u4e0d\u53d7\u64fe\u7684\u5bc6\u5c01\u904b\u884c\u6642\u7528\u87ba\u65cb\u84cb\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6eab\u5ea6\u5c0d\u6211\u7684 UV-Vis \u91cf\u6e2c\u5f71\u97ff\u591a\u5c11\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5178\u578b\u751f\u7269\u6a23\u54c1\u6bcf\u8b8a\u5316 10 \u651d\u6c0f\u5ea6\u986f\u793a 0.5 \u5230 2% \u7684\u5438\u5149\u5ea6\u8b8a\u5316\u3002\u6548\u61c9\u4f86\u81ea\u6298\u5c04\u7387\u8b8a\u5316\uff08\u6bcf\u5ea6\u7d04 0.05%\uff09\u3001\u6a23\u54c1\u9ad4\u7a4d\u81a8\u8139\uff08\u6c34\u6bcf\u5ea6\u81a8\u8139 0.025%\uff09\uff0c\u4ee5\u53ca\u86cb\u767d\u8cea\u8207\u6838\u9178\u7684\u5206\u5b50\u69cb\u8c61\u8b8a\u5316\u3002\u5c0d\u5df2\u77e5\u5ba4\u6eab\u4e0b\u7684\u4f8b\u884c\u7d42\u9ede\u91cf\u6e2c\uff0c\u9019\u53ef\u5ffd\u7565\u3002\u5c0d\u85e5\u5178\u65b9\u6cd5\u3001GMP IPC\uff0c\u8207\u4efb\u4f55\u5169\u4f4d\u6709\u6548\u6578\u5b57\u7cbe\u5ea6\u8981\u7dca\u7684\u5b9a\u91cf\u5de5\u4f5c\uff0c\u7528 Peltier \u6216\u6c34\u593e\u5957\u5ea7\u628a\u6eab\u5ea6\u9396\u5b9a\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6bd4\u8272\u76bf\u6750\u6599\u6703\u5f71\u97ff\u71b1\u97ff\u61c9\u6642\u9593\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u6703\uff0c\u4f46\u5f88\u5f31\u3002\u7194\u878d\u77f3\u82f1\u5c0e\u71b1\u4fc2\u6578 1.4 W\/m\u00b7K\uff0c\u76f8\u8f03\u5149\u5b78\u73bb\u7483 1.1\u3001PMMA 0.19\u3002\u8584\u58c1\u77f3\u82f1\u6bd4\u8272\u76bf\uff081 mm \u58c1\uff09\u5f9e 25 \u5230 65 \u00b0C \u7d04 30 \u79d2\u5e73\u8861\uff1b\u6a19\u6e96 1.5 mm \u58c1\u7d04 90 \u79d2\uff1b\u539a\u58c1\u5149\u5b78\u73bb\u7483\uff083 mm \u58c1\uff09\u7d04 4 \u5206\u9418\u3002\u5c0d\u6bcf\u5206\u9418 0.5 \u00b0C \u7684 DNA \u7194\u89e3\uff0c\u5e73\u8861\u6642\u9593\u76f8\u5c0d\u6383\u63cf\u6b65\u5f88\u77ed\u3002\u5c0d\u5e73\u8861\u662f\u9650\u5236\u6b65\u9a5f\u7684\u5feb\u901f\u52d5\u529b\u5b78\u5de5\u4f5c\uff0c\u9078\u8584\u58c1\u7194\u878d\u77f3\u82f1\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u80fd\u7528\u5851\u81a0\u6bd4\u8272\u76bf\u505a DNA \u7194\u89e3\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u4e0d\u884c\u3002PMMA \u5728 80 \u5230 100 \u00b0C \u8edf\u5316\u3001\u5230 70 \u00b0C \u5c31\u986f\u51fa\u770b\u5f97\u898b\u7684\u8b8a\u5f62\uff1b\u805a\u82ef\u4e59\u70ef\u5230 90 \u00b0C\u3002\u5169\u7a2e\u6750\u6599\u53d7\u71b1\u90fd\u6539\u8b8a\u5c3a\u5bf8\u3001\u4f7f\u5149\u7a0b\u504f\u5dee\u3002\u805a\u78b3\u9178\u916f\u71b1\u7a69\u5b9a\u6027\u8f03\u597d\uff08Tg \u7d04 140 \u00b0C\uff09\uff0c\u4f46\u5f88\u5c11\u7528\u65bc\u7194\u89e3\u5de5\u4f5c\uff0c\u56e0\u70ba\u5b83\u5728 290 nm \u4ee5\u4e0b\u7684\u7d2b\u5916\u900f\u5c04\u5f88\u5dee\u3001\u800c\u77f3\u82f1\u662f\u5df2\u767c\u8868\u65b9\u6cd5\u88e1\u9a57\u8b49\u904e\u7684\u6750\u6599\u3002DNA \u7194\u89e3\u7528\u7194\u878d\u77f3\u82f1\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">Peltier \u6bd4\u8272\u76bf\u5ea7\u6db5\u84cb\u4ec0\u9ebc\u6eab\u5ea6\u7bc4\u570d\uff1f<\/div><div class=\"csg-faq-a\"><p>\u73fe\u4ee3 Peltier \u914d\u4ef6\u5ea7\u901a\u5e38\u6db5\u84cb 5 \u5230 100 \u00b0C\u3001\u7a69\u5b9a\u6027\u6b63\u8ca0 0.1 \u00b0C\u3002\u67d0\u4e9b\u7279\u6b8a Peltier \u6a21\u7d44\u4ee5\u4e3b\u52d5\u51b7\u537b\u5ef6\u4f38\u5230\u8ca0 10 \u00b0C\u3001\u6216\u4ee5\u9ad8\u96fb\u6d41\u914d\u7f6e\u5230 110 \u00b0C\u3002\u8d85\u904e\u6b64\u7bc4\u570d\uff0c\u6c34\u593e\u5957\u5ea7\uff08\u914d\u9069\u7576\u6d41\u9ad4\u8ca0 10 \u5230\u6b63 120 \u00b0C\uff09\u6216\u52a0\u71b1\u584a\u5ea7\uff0825 \u5230 200 \u00b0C\uff09\u624d\u662f\u5c0d\u7684\u9078\u64c7\u3002\u67e5\u4f60\u5149\u8b5c\u5100\u5ee0\u5546\u7684\u914d\u4ef6\u898f\u683c\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u52d5\u529b\u5b78\u5de5\u4f5c\u9700\u8981\u5728\u6bd4\u8272\u76bf\u88e1\u653e\u78c1\u652a\u62cc\u5668\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5c0d\u591a\u6578\u5ba4\u6eab\u3001\u8a66\u5291\u6df7\u5408\u826f\u597d\u7684\u9175\u7d20\u52d5\u529b\u5b78\uff0c\u4e0d\u7528\u3002\u6bd4\u8272\u76bf\u9ad4\u7a4d\uff08\u6a19\u6e96 3.5 mL\uff09\u5920\u5c0f\uff0c\u64f4\u6563\u5728 1 \u5230 2 \u5206\u9418\u5167\u5b8c\u6210\u6df7\u5408\u3002\u5c0d\u6df7\u5408\u662f\u9650\u901f\u6b65\u9a5f\u7684\u53cd\u61c9\uff08\u975e\u5e38\u5feb\u7684\u9175\u7d20\u3001\u9ecf\u7a20\u6a23\u54c1\u3001\u9846\u7c92\u61f8\u6d6e\u6db2\uff09\uff0c\u6bd4\u8272\u76bf\u88e1\u7684\u78c1\u652a\u62cc\u5b50\u52a0\u6bd4\u8272\u76bf\u5ea7\u88e1\u7684\u652a\u62cc\u5668\u914d\u4ef6\u6d88\u9664\u64f4\u6563\u5ef6\u9072\u3002\u6709\u4e9b\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u8a2d\u8a08\u4f86\u5bb9\u7d0d\u5c0f\u652a\u62cc\u5b50\uff1b\u82e5\u9700\u8981\u652a\u62cc\uff0c\u4e0b\u55ae\u524d\u78ba\u8a8d\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u80fd\u7528 NanoDrop \u5e95\u5ea7\u505a\u9175\u7d20\u52d5\u529b\u5b78\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u4e0d\u884c\u3002NanoDrop \u5e95\u5ea7\u662f\u55ae\u9ede\u91cf\u6e2c\u5100\u5668\u2014\u2014\u79fb\u6db2\u3001\u91cf\u6e2c\u3001\u64e6\u62ed\u3002\u5b83\u5011\u4e0d\u652f\u63f4\u9023\u7e8c\u76e3\u6e2c\u52d5\u529b\u5b78\u6a21\u5f0f\u3001\u4e5f\u6c92\u6709\u9069\u5408\u52d5\u529b\u5b78\u5de5\u4f5c\u7684\u63a7\u6eab\u3002\u9175\u7d20\u52d5\u529b\u5b78\u8acb\u7528\u5e36 Peltier \u6216\u6c34\u593e\u5957\u6bd4\u8272\u76bf\u5ea7\u8207\u5bc6\u5c01\u6bd4\u8272\u76bf\u7684 UV-Vis \u684c\u4e0a\u578b\u5206\u5149\u5149\u5ea6\u8a08\u3002\u6bd4\u8f03\u898b\u6211\u5011\u7684\u6bd4\u8272\u76bf vs NanoDrop \u6307\u5357\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">Peltier \u5ea7\u9054\u5230\u65b0\u6eab\u5ea6\u8981\u591a\u4e45\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5178\u578b Peltier \u6a21\u7d44\u4ee5\u6bcf\u5206\u9418 1 \u5230 5 \u00b0C \u5347\u964d\u3002\u9054\u5230\u65b0\u8a2d\u5b9a\u9ede\u7684\u6b63\u8ca0 0.5 \u00b0C \u9700 5 \u5230 30 \u5206\u9418\u3001\u8996\u6eab\u5dee\u800c\u5b9a\u3002\u4e00\u5230\u8a2d\u5b9a\u9ede\uff0c\u7a69\u5b9a\u6027\u7121\u9650\u671f\u7dad\u6301\u6b63\u8ca0 0.1 \u00b0C\u3002\u5c0d DNA \u7194\u89e3\u5de5\u4f5c\u6d41\u7a0b\uff0c0.1 \u5230 5 \u00b0C\/min \u7684\u6383\u63cf\u901f\u7387\u9078\u9805\u662f\u5178\u578b\u7684\uff1b0.5 \u5230 1.0 \u00b0C\/min \u662f\u92b3\u5229 Tm \u5224\u5b9a\u7684\u751c\u871c\u9ede\u3002\u66f4\u5feb\u7684\u6383\u63cf\u7d66\u51fa\u7684 Tm \u4f30\u8a08\u5e36\u7d04 1 \u5230 2 \u00b0C \u671d\u8f03\u9ad8\u6eab\u5ea6\u7684\u7cfb\u7d71\u6027\u8aa4\u5dee\uff08\u52d5\u529b\u5b78\u6eef\u5f8c\uff09\u3002<\/p><\/div><\/div>\n<\/div><h2 id=\"disclaimer\">12. \u514d\u8cac\u8072\u660e\u8207\u8a3b\u8a18<\/h2>\n<div class=\"csg-callout\">\n<p><strong>\u5efa\u8b70<\/strong> \u672c\u9801\u4e0a\u7684\u662f\u91dd\u5c0d\u5178\u578b UV-Vis \u63a7\u6eab\u5de5\u4f5c\u7684\u4e00\u822c\u6307\u5f15\u3002\u7279\u5b9a\u6e2c\u5b9a\u3001\u6cd5\u898f\u74b0\u5883\u6216\u5100\u5668\u5ee0\u5546\u5efa\u8b70\u53ef\u80fd\u6539\u8b8a\u9078\u64c7\u3002\u5c0d\u85e5\u5178\u65b9\u6cd5\uff08USP\u3001EP\u3001JP\uff09\u9075\u5faa\u5df2\u767c\u8868\u65b9\u6cd5\u88e1\u7684\u6bd4\u8272\u76bf\u898f\u683c\u3002<\/p>\n<p><strong>\u6eab\u5ea6\u7bc4\u570d\u8207\u7a69\u5b9a\u6027\u503c<\/strong> \u91dd\u5c0d Peltier \u8207\u6c34\u593e\u5957\u5ea7\u7684\u662f\u5beb\u4f5c\u7576\u6642\u73fe\u4ee3\u5100\u5668\u7684\u5178\u578b\u3002\u6c7a\u5b9a\u6027\u7684\u6578\u503c\u5411\u4f60\u5206\u5149\u5149\u5ea6\u8a08\u5ee0\u5546\u7684\u914d\u4ef6\u898f\u683c\u9a57\u8b49\u3002<\/p>\n<p><strong>\u6c34\u593e\u5957\u6bd4\u8272\u76bf vs \u5ea7\u3002<\/strong> MachinedQuartz \u88fd\u9020\u88dd\u5f97\u9032\u6c34\u593e\u5957\u6bd4\u8272\u76bf\u5ea7\u7684\u6a19\u6e96\u6bd4\u8272\u76bf\u3002\u6211\u5011\u4e0d\u88fd\u9020\u4e00\u9ad4\u5f0f\u6c34\u593e\u5957\u6bd4\u8272\u76bf\uff08\u5e36\u710a\u63a5\u5916\u6c34\u593e\u5957\u7684\u6bd4\u8272\u76bf\uff09\u2014\u2014\u90a3\u7a2e\u7279\u6b8a\u898f\u683c\u8acb\u6d3d Hellma 121 \u7cfb\u5217\u6216\u540c\u7d1a\u54c1\u3002\u672c\u6307\u5357\u63cf\u8ff0\u7684 Peltier \u52a0\u6a19\u6e96\u6bd4\u8272\u76bf\u5de5\u4f5c\u6d41\u7a0b\u662f\u4e3b\u5c0e\u7684\u73fe\u4ee3\u505a\u6cd5\u3001\u6db5\u84cb 95% \u7684\u63a7\u6eab UV-Vis \u5de5\u4f5c\u3002<\/p>\n<p><strong>\u5546\u6a19\u8072\u660e\uff1a<\/strong> Cary\u3001Lambda\u3001Shimadzu\u3001JASCO\u3001OLIS\u3001Aviv\u3001Hellma\u3001Hamilton \u662f\u5404\u81ea\u64c1\u6709\u8005\u7684\u5546\u6a19\u3002\u5f15\u7528\u50c5\u70ba\u76f8\u5bb9\u6027\u8207\u65b9\u6cd5\u8108\u7d61\u3002<\/p>\n<p><strong>\u8cc7\u8a0a\u6642\u6548\uff1a<\/strong> \u6700\u5f8c\u5be9\u95b1 2026 \u5e74 5 \u6708\u3002<\/p>\n<\/div><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Article\",\n  \"headline\": \"\u7528\u65bc DNA \u7194\u89e3\u8207\u9175\u7d20\u52d5\u529b\u5b78\u7684\u6046\u6eab\u6bd4\u8272\u76bf\",\n  \"description\": \"\u63a7\u6eab UV-Vis \u6bd4\u8272\u76bf\uff1aDNA \u7194\u89e3(25\u201395\u00b0C)\u3001\u9175\u7d20\u52d5\u529b\u5b78\u3001\u505c\u6d41\u30023 \u7a2e\u5bc6\u5c01\u5c64\u7d1a\u3001Peltier\/\u6c34\u593e\u5957\/\u52a0\u71b1\u584a\u3001\u71b1\u97ff\u61c9\u8207\u6c7a\u7b56\u77e9\u9663\u3002\",\n  \"author\": {\n    \"@type\": \"Organization\",\n    \"name\": \"MachinedQuartz\"\n  },\n  \"publisher\": {\n    \"@type\": \"Organization\",\n    \"name\": \"MachinedQuartz\",\n    \"url\": \"https:\/\/machinedquartz.com\"\n  },\n  \"datePublished\": \"2026-01-17\",\n  \"dateModified\": \"2026-01-19\",\n  \"mainEntityOfPage\": \"https:\/\/machinedquartz.com\/zh-hant\/thermostatted-cuvettes-dna-melting-enzyme-kinetics\/\",\n  \"inLanguage\": \"zh-Hant\"\n}\n<\/script><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"DNA \u7194\u89e3\u66f2\u7dda\u9700\u8981\u7279\u6b8a\u7684\u6bd4\u8272\u76bf\u55ce\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u662f\uff0c\u610f\u601d\u662f\u6bd4\u8272\u76bf\u5728\u6574\u500b\u6eab\u5ea6\u6383\u63cf\u671f\u9593\u5fc5\u9808\u5bc6\u5c01\u3002DNA \u5f9e 25 \u5230 95 \u00b0C \u7194\u89e3\u901a\u5e38\u8981 30 \u5230 90 \u5206\u9418\uff1b\u958b\u653e\u6216 PTFE \u6469\u64e6\u84cb\u6bd4\u8272\u76bf\u5728\u904b\u884c\u4e2d\u84b8\u767c\u640d\u5931 3 \u5230 8% \u7684\u9ad4\u7a4d\uff0c\u6fc3\u7e2e DNA \u4e26\u4f7f\u8868\u89c0 A260 \u57fa\u7dda\u504f\u5dee\u3002\u9644 PTFE \u5167\u896f\u7684\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u662f\u6a19\u6e96\u9078\u64c7\u3002\u5916\u90e8\u5c3a\u5bf8\u3001\u5149\u5b78\u5e7e\u4f55\uff08\u96d9\u9762\u6216\u56db\u9762\u900f\u5149\uff09\u8207\u5149\u7a0b\uff08\u5178\u578b 10 mm\uff09\u5247\u8207\u4efb\u4f55\u5176\u4ed6 UV-Vis \u6bd4\u8272\u76bf\u76f8\u540c\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"\u6211\u80fd\u5728 Peltier \u63a7\u6eab\u6bd4\u8272\u76bf\u5ea7\u88e1\u7528\u6a19\u6e96 10 mm \u6bd4\u8272\u76bf\u55ce\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u53ef\u4ee5\u3002Peltier \u5ea7\u8a2d\u8a08\u4f86\u63a5\u53d7\u901a\u7528\u7684 12.5 \u00d7 12.5 \u00d7 45 mm \u5916\u90e8\u5c3a\u5bf8\u6bd4\u8272\u76bf\u3002Peltier \u5143\u4ef6\u900f\u904e\u5ea7\u7684\u91d1\u5c6c\u58c1\u8207\u6bd4\u8272\u76bf\u672c\u9ad4\u505a\u71b1\u63a5\u89f8\u3002\u4e00\u652f\u6a19\u6e96 MachinedQuartz 10 mm \u6bd4\u8272\u76bf\u5728\u4efb\u4f55\u73fe\u4ee3 Peltier \u5ea7\u88e1\u90fd\u88dd\u5f97\u9032\u3001\u4e5f\u80fd\u7528\u3002\u9650\u5236\u662f\u6bd4\u8272\u76bf\u5bc6\u5c01\u3001\u4e0d\u662f\u5e7e\u4f55\uff1a\u5c0d\u5728\u8207\u74b0\u5883\u4e0d\u540c\u6eab\u5ea6\u4e0b\u8d85\u904e\u5e7e\u5206\u9418\u7684\u904b\u884c\uff0c\u7528\u87ba\u65cb\u84cb\u6216\u52a0\u585e\u6bd4\u8272\u76bf\u4ee5\u9632\u84b8\u767c\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"\u52a0\u585e\u6bd4\u8272\u76bf\u8207\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u5dee\u5728\u54ea\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u52a0\u585e\u6bd4\u8272\u76bf\u6709\u4e00\u500b\u914d\u5728\u6bd4\u8272\u76bf\u672c\u9ad4\u78e8\u7802\u73bb\u7483\u627f\u5ea7\u4e0a\u7684 PTFE \u6216\u73bb\u7483\u585e\u2014\u2014\u6469\u64e6\u914d\u5408\u3001\u5feb\u901f\u63d2\u5165\u8207\u53d6\u51fa\u300130 \u5206\u9418\u5167\u5bc6\u5c01\u8868\u73fe\u7d04 95%\u3002\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u6709\u4e00\u500b\u9644 PTFE \u5167\u896f\u7684\u87ba\u7d0b\u84cb\u2014\u2014\u56e0\u70ba\u8981\u9396\u87ba\u7d0b\u6240\u4ee5\u63d2\u5165\u8f03\u6162\u3001\u591a\u5c0f\u6642\u904b\u884c\u5bc6\u5c01\u8868\u73fe\u8d85\u904e 99%\uff0c\u5c0d DNA \u7194\u89e3\u3001\u53ad\u6c27\u5de5\u4f5c\u8207\u4efb\u4f55\u63ee\u767c\u6eb6\u5291\u61c9\u7528\u90fd\u5fc5\u5099\u3002\u9700\u8981\u5728\u5be6\u9a57\u4e2d\u52a0\u6a23\u6642\u7528\u52a0\u585e\uff1b\u9700\u8981\u9577\u6642\u9593\u3001\u4e0d\u53d7\u64fe\u7684\u5bc6\u5c01\u904b\u884c\u6642\u7528\u87ba\u65cb\u84cb\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"\u6eab\u5ea6\u5c0d\u6211\u7684 UV-Vis \u91cf\u6e2c\u5f71\u97ff\u591a\u5c11\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u5178\u578b\u751f\u7269\u6a23\u54c1\u6bcf\u8b8a\u5316 10 \u651d\u6c0f\u5ea6\u986f\u793a 0.5 \u5230 2% 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\u5230 100 \u00b0C \u8edf\u5316\u3001\u5230 70 \u00b0C \u5c31\u986f\u51fa\u770b\u5f97\u898b\u7684\u8b8a\u5f62\uff1b\u805a\u82ef\u4e59\u70ef\u5230 90 \u00b0C\u3002\u5169\u7a2e\u6750\u6599\u53d7\u71b1\u90fd\u6539\u8b8a\u5c3a\u5bf8\u3001\u4f7f\u5149\u7a0b\u504f\u5dee\u3002\u805a\u78b3\u9178\u916f\u71b1\u7a69\u5b9a\u6027\u8f03\u597d\uff08Tg \u7d04 140 \u00b0C\uff09\uff0c\u4f46\u5f88\u5c11\u7528\u65bc\u7194\u89e3\u5de5\u4f5c\uff0c\u56e0\u70ba\u5b83\u5728 290 nm \u4ee5\u4e0b\u7684\u7d2b\u5916\u900f\u5c04\u5f88\u5dee\u3001\u800c\u77f3\u82f1\u662f\u5df2\u767c\u8868\u65b9\u6cd5\u88e1\u9a57\u8b49\u904e\u7684\u6750\u6599\u3002DNA \u7194\u89e3\u7528\u7194\u878d\u77f3\u82f1\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Peltier \u6bd4\u8272\u76bf\u5ea7\u6db5\u84cb\u4ec0\u9ebc\u6eab\u5ea6\u7bc4\u570d\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u73fe\u4ee3 Peltier \u914d\u4ef6\u5ea7\u901a\u5e38\u6db5\u84cb 5 \u5230 100 \u00b0C\u3001\u7a69\u5b9a\u6027\u6b63\u8ca0 0.1 \u00b0C\u3002\u67d0\u4e9b\u7279\u6b8a Peltier \u6a21\u7d44\u4ee5\u4e3b\u52d5\u51b7\u537b\u5ef6\u4f38\u5230\u8ca0 10 \u00b0C\u3001\u6216\u4ee5\u9ad8\u96fb\u6d41\u914d\u7f6e\u5230 110 \u00b0C\u3002\u8d85\u904e\u6b64\u7bc4\u570d\uff0c\u6c34\u593e\u5957\u5ea7\uff08\u914d\u9069\u7576\u6d41\u9ad4\u8ca0 10 \u5230\u6b63 120 \u00b0C\uff09\u6216\u52a0\u71b1\u584a\u5ea7\uff0825 \u5230 200 \u00b0C\uff09\u624d\u662f\u5c0d\u7684\u9078\u64c7\u3002\u67e5\u4f60\u5149\u8b5c\u5100\u5ee0\u5546\u7684\u914d\u4ef6\u898f\u683c\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"\u52d5\u529b\u5b78\u5de5\u4f5c\u9700\u8981\u5728\u6bd4\u8272\u76bf\u88e1\u653e\u78c1\u652a\u62cc\u5668\u55ce\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u5c0d\u591a\u6578\u5ba4\u6eab\u3001\u8a66\u5291\u6df7\u5408\u826f\u597d\u7684\u9175\u7d20\u52d5\u529b\u5b78\uff0c\u4e0d\u7528\u3002\u6bd4\u8272\u76bf\u9ad4\u7a4d\uff08\u6a19\u6e96 3.5 mL\uff09\u5920\u5c0f\uff0c\u64f4\u6563\u5728 1 \u5230 2 \u5206\u9418\u5167\u5b8c\u6210\u6df7\u5408\u3002\u5c0d\u6df7\u5408\u662f\u9650\u901f\u6b65\u9a5f\u7684\u53cd\u61c9\uff08\u975e\u5e38\u5feb\u7684\u9175\u7d20\u3001\u9ecf\u7a20\u6a23\u54c1\u3001\u9846\u7c92\u61f8\u6d6e\u6db2\uff09\uff0c\u6bd4\u8272\u76bf\u88e1\u7684\u78c1\u652a\u62cc\u5b50\u52a0\u6bd4\u8272\u76bf\u5ea7\u88e1\u7684\u652a\u62cc\u5668\u914d\u4ef6\u6d88\u9664\u64f4\u6563\u5ef6\u9072\u3002\u6709\u4e9b\u87ba\u65cb\u84cb\u6bd4\u8272\u76bf\u8a2d\u8a08\u4f86\u5bb9\u7d0d\u5c0f\u652a\u62cc\u5b50\uff1b\u82e5\u9700\u8981\u652a\u62cc\uff0c\u4e0b\u55ae\u524d\u78ba\u8a8d\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"\u6211\u80fd\u7528 NanoDrop \u5e95\u5ea7\u505a\u9175\u7d20\u52d5\u529b\u5b78\u55ce\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u4e0d\u884c\u3002NanoDrop \u5e95\u5ea7\u662f\u55ae\u9ede\u91cf\u6e2c\u5100\u5668\u2014\u2014\u79fb\u6db2\u3001\u91cf\u6e2c\u3001\u64e6\u62ed\u3002\u5b83\u5011\u4e0d\u652f\u63f4\u9023\u7e8c\u76e3\u6e2c\u52d5\u529b\u5b78\u6a21\u5f0f\u3001\u4e5f\u6c92\u6709\u9069\u5408\u52d5\u529b\u5b78\u5de5\u4f5c\u7684\u63a7\u6eab\u3002\u9175\u7d20\u52d5\u529b\u5b78\u8acb\u7528\u5e36 Peltier \u6216\u6c34\u593e\u5957\u6bd4\u8272\u76bf\u5ea7\u8207\u5bc6\u5c01\u6bd4\u8272\u76bf\u7684 UV-Vis \u684c\u4e0a\u578b\u5206\u5149\u5149\u5ea6\u8a08\u3002\u6bd4\u8f03\u898b\u6211\u5011\u7684\u6bd4\u8272\u76bf vs NanoDrop \u6307\u5357\u3002\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Peltier \u5ea7\u9054\u5230\u65b0\u6eab\u5ea6\u8981\u591a\u4e45\uff1f\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"\u5178\u578b Peltier \u6a21\u7d44\u4ee5\u6bcf\u5206\u9418 1 \u5230 5 \u00b0C \u5347\u964d\u3002\u9054\u5230\u65b0\u8a2d\u5b9a\u9ede\u7684\u6b63\u8ca0 0.5 \u00b0C \u9700 5 \u5230 30 \u5206\u9418\u3001\u8996\u6eab\u5dee\u800c\u5b9a\u3002\u4e00\u5230\u8a2d\u5b9a\u9ede\uff0c\u7a69\u5b9a\u6027\u7121\u9650\u671f\u7dad\u6301\u6b63\u8ca0 0.1 \u00b0C\u3002\u5c0d DNA \u7194\u89e3\u5de5\u4f5c\u6d41\u7a0b\uff0c0.1 \u5230 5 \u00b0C\/min \u7684\u6383\u63cf\u901f\u7387\u9078\u9805\u662f\u5178\u578b\u7684\uff1b0.5 \u5230 1.0 \u00b0C\/min \u662f\u92b3\u5229 Tm \u5224\u5b9a\u7684\u751c\u871c\u9ede\u3002\u66f4\u5feb\u7684\u6383\u63cf\u7d66\u51fa\u7684 Tm \u4f30\u8a08\u5e36\u7d04 1 \u5230 2 \u00b0C \u671d\u8f03\u9ad8\u6eab\u5ea6\u7684\u7cfb\u7d71\u6027\u8aa4\u5dee\uff08\u52d5\u529b\u5b78\u6eef\u5f8c\uff09\u3002\"\n      }\n    }\n  ],\n  \"inLanguage\": \"zh-Hant\"\n}\n<\/script><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"BreadcrumbList\",\n  \"itemListElement\": [\n    {\n      \"@type\": \"ListItem\",\n      \"position\": 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