{"id":98671,"date":"2025-11-21T10:00:00","date_gmt":"2025-11-21T02:00:00","guid":{"rendered":"https:\/\/machinedquartz.com\/cuvette-spectrophotometer-verification-usp-ep\/"},"modified":"2026-06-12T09:35:56","modified_gmt":"2026-06-12T01:35:56","slug":"cuvette-spectrophotometer-verification-usp-ep","status":"publish","type":"post","link":"https:\/\/machinedquartz.com\/zh-hant\/cuvette-spectrophotometer-verification-usp-ep\/","title":{"rendered":"\u6bd4\u8272\u76bf\u8207\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\uff1aUSP\u3001EP \u8207 GMP \u898f\u7a0b"},"content":{"rendered":"\n<div class=\"cq-aidef\" style=\"background:#fafbff;border:1px solid #e0e7ff;border-radius:10px;padding:18px 22px;margin:0 0 24px;\"><p style=\"margin:0;font-size:16px;line-height:1.65;color:#1e293b;\"><strong style=\"color:#1a2a6c;\">\u4f9d USP <851> \u8207 EP \u7684\u6bd4\u8272\u76bf\u8207\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\u662f<\/strong> \u5c0d UV-Vis \u5100\u5668\u53ca\u5176\u6bd4\u8272\u76bf\u7684\u3001\u85e5\u5178\u898f\u5b9a\u7684\u5b63\u5ea6\u78ba\u6548\uff0c\u7528\u65bc\u88fd\u85e5\u54c1\u7ba1\u4ee5\u78ba\u8a8d\u6ce2\u9577\u6e96\u78ba\u5ea6\uff08\u00b11 nm\uff09\u3001\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\uff08A = 1 \u6642 \u00b10.005 AU\uff09\u3001\u96dc\u6563\u5149\uff08< 0.01% \u5728 200 nm \u8655\uff09\uff0c\u4ee5\u53ca\u89e3\u6790\u5ea6\uff08\u2264 2 nm \u983b\u5bec\uff09\u3002\u672c\u898f\u7a0b\u4f7f\u7528\u8a8d\u8b49\u53c3\u8003\u7269\u8cea\uff08\u91cd\u927b\u9178\u9240\u3001\u9420\u91f9\u6ffe\u7247\u3001NIST SRM\uff09\u8207\u5177\u5099\u6587\u4ef6\u5316\u5149\u5b78\u7b49\u6548\u6027\u7684\u914d\u5c0d\u77f3\u82f1\u6bd4\u8272\u76bf\u3002<\/p><\/div>\n\n\n<div class=\"csg-page\"><style>\n.csg-page { max-width:880px; margin:0 auto; padding:0 18px; color:#333; line-height:1.65; font-size:16px; }\n.csg-page p, .csg-page li { color:#333; }\n.csg-page h2 { font-size:clamp(22px,2.2vw,28px); font-weight:700; color:#1a1a2e; 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}\n.csg-toc-floating ol { list-style:none !important; padding:0 !important; margin:0; counter-reset:toc; }\n.csg-toc-floating ol li { list-style:none !important; counter-increment:toc; padding:6px 0 6px 28px !important; position:relative; line-height:1.35; border-bottom:1px dashed #eef0f6; transition:background 0.15s, border-radius 0.15s; }\n.csg-toc-floating ol li:last-child { border-bottom:none; }\n.csg-toc-floating ol li::before { content:counter(toc); position:absolute; left:0; top:6px; width:22px; height:22px; background:#f1f4fb; color:#233a95; border-radius:50%; font-size:11px; font-weight:700; display:flex; align-items:center; justify-content:center; transition:background 0.15s, color 0.15s; }\n.csg-toc-floating ol li a { color:#1a1a2e; text-decoration:none; transition:color 0.15s, font-weight 0.15s; }\n.csg-toc-floating ol li a:hover { color:#233a95; }\n.csg-toc-floating ol li.csg-toc-active { background:linear-gradient(90deg,#eef1fb 0%,rgba(238,241,251,0.4) 100%); 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border-left-color:#7c3aed; background:#f5f3ff; }\n.csg-callout strong { color:#1a1a2e; }\n.csg-eeat-box { background:#f7f8fc; border-left:4px solid #16a34a; padding:16px 20px; margin:24px 0; border-radius:6px; font-size:14px; }\n.csg-eeat-box strong { color:#16a34a; }\n.csg-cta-box { background:linear-gradient(135deg,#1a2a6c,#233a95); border-radius:12px; padding:28px 32px; margin:32px 0; text-align:center; color:#fff !important; }\n.csg-cta-box h3 { color:#fff !important; margin:0 0 8px; }\n.csg-cta-box p { color:#d6e0ff !important; margin:0 0 16px; }\n.csg-cta-btn { display:inline-block; background:#fff; color:#1a2a6c !important; padding:12px 26px; border-radius:6px; font-weight:700; text-decoration:none !important; margin:4px 6px; }\n.csg-cta-btn:hover { background:#f1f4fb; }\n.csg-cta-btn.outline { background:transparent; color:#fff !important; border:2px solid #fff; }\n.csg-cta-btn.outline:hover { background:rgba(255,255,255,0.12); }\n.csg-faq { margin:24px 0; }\n.csg-faq-item { border-bottom:1px solid #e6e9f5; }\n.csg-faq-q { display:flex; justify-content:space-between; align-items:center; padding:16px 0; cursor:pointer; font-weight:600; color:#1a1a2e; font-size:16px; }\n.csg-faq-q::after { content:\"+\"; font-size:22px; color:#233a95; font-weight:400; }\n.csg-faq-item.open .csg-faq-q::after { content:\"-\"; }\n.csg-faq-a { max-height:0; overflow:hidden; transition:max-height 0.3s ease; color:#555; line-height:1.65; }\n.csg-faq-item.open .csg-faq-a { max-height:1200px; padding-bottom:18px; }\n.csg-dim-grid { display:grid; grid-template-columns:repeat(5,1fr); gap:10px; margin:24px 0; }\n.csg-dim-card { background:#fff; border:1px solid #e0e4ef; border-radius:10px; padding:16px 12px; text-align:center; transition:transform 0.15s, box-shadow 0.15s; }\n.csg-dim-card:hover { transform:translateY(-3px); box-shadow:0 6px 18px rgba(35,58,149,0.10); }\n.csg-dim-card .csg-dim-icon { font-size:26px; line-height:1; display:block; margin-bottom:6px; }\n.csg-dim-card h4 { font-size:13px; font-weight:700; color:#1a2a6c; margin:6px 0 4px; }\n.csg-dim-card p { font-size:11px; color:#666; line-height:1.45; margin:0; }\n@media (max-width:720px) { .csg-dim-grid { grid-template-columns:repeat(2,1fr); } }\n.csg-related-grid { display:grid; grid-template-columns:repeat(2,1fr); gap:14px; margin:24px 0; }\n.csg-related-card { background:#fff; border:1px solid #e0e4ef; border-radius:10px; padding:16px 18px; text-decoration:none !important; color:#333 !important; transition:background 0.18s, border-color 0.18s, transform 0.15s, box-shadow 0.18s; display:block; }\n.csg-related-card:hover { background:#f7f8fc; border-color:#233a95; transform:translateY(-2px); box-shadow:0 6px 18px rgba(35,58,149,0.10); }\n.csg-related-icon { font-size:24px; line-height:1; display:block; margin-bottom:6px; }\n.csg-related-h { display:block; font-size:15px; font-weight:700; color:#1a2a6c !important; margin-bottom:4px; }\n.csg-related-d { display:block; font-size:13px; color:#555; line-height:1.45; }\n@media (max-width:720px) { .csg-related-grid { grid-template-columns:1fr; } }\n<\/style><section class=\"csg-hero\">\n<div class=\"csg-hero-eyebrow\">USP <851> \u00b7 EP 2.2.25 \u00b7 \u8207 ICH \u4e00\u81f4\u7684\u65b9\u6cd5<\/div>\n<p class=\"csg-hero-h1\">\u6bd4\u8272\u76bf\u8207\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\uff1aUSP\u3001EP \u8207 GMP \u898f\u7a0b<\/p>\n<p class=\"csg-hero-dek\">\u4e00\u4efd\u5be6\u52d9\u8005\u6307\u5357\uff0c\u8b1b\u6bcf\u500b\u53d7\u898f\u7bc4 UV-Vis \u5be6\u9a57\u5ba4\u90fd\u5fc5\u9808\u78ba\u6548\u7684\u4e94\u500b\u9a57\u8b49\u7dad\u5ea6\u2014\u2014\u6ce2\u9577\u6e96\u78ba\u5ea6\u3001\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u3001\u5149\u5ea6\u7dda\u6027\u3001\u96dc\u6563\u5149\u8207\u89e3\u6790\u5ea6\u2014\u2014\u518d\u52a0\u4e0a\u7a3d\u6838\u54e1\u771f\u6b63\u6703\u770b\u7684\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5\uff08\u5149\u7a0b\u3001\u914d\u5c0d\u5339\u914d\u3001\u7a97\u7247\u5e73\u6574\u5ea6\uff09\u3002<\/p>\n<div class=\"csg-stats\">\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">5<\/div><div class=\"csg-stat-lbl\">\u9a57\u8b49\u7dad\u5ea6<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">3<\/div><div class=\"csg-stat-lbl\">\u6bd4\u8272\u76bf\u6aa2\u67e5<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">USP <851><\/div><div class=\"csg-stat-lbl\">\u65b9\u6cd5\u53c3\u8003<\/div><\/div>\n<div class=\"csg-stat\"><div class=\"csg-stat-num\">10 \u500b SOP<\/div><div class=\"csg-stat-lbl\">\u5f15\u7528\u7684\u7bc4\u672c<\/div><\/div>\n<\/div>\n<\/section><aside class=\"csg-toc-floating\" id=\"csg-toc\">\n<button class=\"csg-toc-handle\" type=\"button\" aria-label=\"Toggle table of contents\" onclick=\"document.getElementById('csg-toc').classList.toggle('collapsed')\"><span class=\"csg-toc-arrow-wrap\"><span class=\"csg-toc-arrow\">\u25b8<\/span><\/span><span class=\"csg-toc-vlabel\">\u672c\u9801\u5167\u5bb9<\/span><\/button>\n<div class=\"csg-toc-content\">\n<div class=\"csg-toc-title\">\u672c\u9801\u5167\u5bb9<\/div>\n<ol>\n<li><a href=\"#why\">\u70ba\u4f55\u9a57\u8b49\u91cd\u8981<\/a><\/li>\n<li><a href=\"#five\">5 \u500b\u9a57\u8b49\u7dad\u5ea6<\/a><\/li>\n<li><a href=\"#wavelength\">\u6ce2\u9577\u6e96\u78ba\u5ea6<\/a><\/li>\n<li><a href=\"#absorbance\">\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6<\/a><\/li>\n<li><a href=\"#linearity\">\u5149\u5ea6\u7dda\u6027<\/a><\/li>\n<li><a href=\"#stray\">\u96dc\u6563\u5149<\/a><\/li>\n<li><a href=\"#resolution\">\u89e3\u6790\u5ea6<\/a><\/li>\n<li><a href=\"#pathlength\">\u6bd4\u8272\u76bf\u5149\u7a0b<\/a><\/li>\n<li><a href=\"#matching\">\u6bd4\u8272\u76bf\u914d\u5c0d\u5339\u914d<\/a><\/li>\n<li><a href=\"#frequency\">\u9a57\u8b49\u983b\u7387<\/a><\/li>\n<li><a href=\"#documentation\">\u6587\u4ef6\u8207 SOP<\/a><\/li>\n<li><a href=\"#standards\">\u53c3\u8003\u7269\u8cea<\/a><\/li>\n<li><a href=\"#faq\">\u5e38\u898b\u554f\u984c<\/a><\/li>\n<li><a href=\"#references\">\u53c3\u8003\u6587\u737b<\/a><\/li>\n<li><a href=\"#disclaimer\">\u514d\u8cac\u8072\u660e<\/a><\/li>\n<\/ol>\n<\/div>\n<\/aside><p>\u6bcf\u53f0 UV-Vis \u5206\u5149\u5149\u5ea6\u8a08\u90fd\u6703\u6f02\u79fb\u3002\u71c8\u8001\u5316\u5728\u516d\u500b\u6708\u5167\u628a\u8868\u89c0\u6ce2\u9577\u4f4d\u79fb 0.1\u20130.3 nm\u3002\u5075\u6e2c\u5668\u96fb\u5b50\u6bcf\u5e74\u8b93\u5149\u5ea6\u6e96\u78ba\u5ea6\u6f02\u79fb 0.5\u20131%\u3002\u6bd4\u8272\u76bf\u7a97\u7247\u6cbe\u4e0a\u8089\u773c\u770b\u4e0d\u898b\u3001\u4f46\u5728\u900f\u5c04\u4e0a\u771f\u5be6\u5b58\u5728\u7684\u8584\u819c\u3001\u522e\u75d5\u8207\u5c0f\u6c59\u67d3\u7279\u5fb5\u3002\u5728\u7814\u7a76\u5be6\u9a57\u5ba4\u9019\u4e9b\u6f02\u79fb\u662f\u9ebb\u7169\uff1b\u5728\u53d7\u898f\u7bc4\u5be6\u9a57\u5ba4\uff08GMP \u88fd\u85e5\u3001GLP \u6bd2\u7406\u3001\u5411 FDA \u7533\u5831\u7684 IVD \u5668\u6750\u3001ISO 17025 \u8a8d\u8b49\u6aa2\u6e2c\uff09\u88e1\uff0c\u5b83\u5011\u662f\u300c\u7d50\u679c\u88ab\u63a5\u53d7\u300d\u8207\u300c\u6279\u6b21\u88ab\u62d2\u300d\u4e4b\u9593\u7684\u5dee\u5225\u2014\u2014\u6216\u662f\u4f60\u4e0b\u6b21\u7a3d\u67e5\u88e1\u7684\u4e00\u689d\u7f3a\u5931\u3002<\/p>\n\n<p>\u85e5\u5178\u7684\u7b54\u6848\u662f\u7d50\u69cb\u5316\u7684\u9031\u671f\u6027\u9a57\u8b49\u3002 <strong>USP <851> \u5206\u5149\u5149\u5ea6\u6cd5\u8207\u5149\u6563\u5c04<\/strong> \u5728\u7f8e\u570b\u3001 <strong>EP 2.2.25 \u7d2b\u5916\u8207\u53ef\u898b\u5438\u6536\u5206\u5149\u5149\u5ea6\u6cd5<\/strong> \u5728\u6b50\u6d32\u3001 <strong>JP 2.24 \u7d2b\u5916-\u53ef\u898b\u5206\u5149\u5149\u5ea6\u6cd5<\/strong> \u5728\u65e5\u672c\uff0c\u4ee5\u53ca <strong>ChP 0401<\/strong> \u5728\u4e2d\u570b\uff0c\u5168\u90fd\u63cf\u8ff0\u4e86\u9a57\u8b49\u6ce2\u9577\u6e96\u78ba\u5ea6\u3001\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u3001\u5149\u5ea6\u7dda\u6027\u3001\u96dc\u6563\u5149\u8207\u89e3\u6790\u5ea6\u7684\u8fd1\u4e4e\u76f8\u540c\u7684\u898f\u7a0b\u3002\u672c\u9801\u6524\u958b\u9019\u4e94\u500b\u7dad\u5ea6\uff0c\u518d\u52a0\u4e0a\u5b8c\u6210\u4e00\u4efd\u7ad9\u5f97\u4f4f\u8173\u7684\u78ba\u6548\u5305\u7684\u4e09\u500b\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5\uff08\u5149\u7a0b\u3001\u914d\u5c0d\u5339\u914d\u3001\u7a97\u7247\u5e73\u6574\u5ea6\uff09\u3002<\/p>\n\n<div class=\"csg-eeat-box\">\n<strong>\u672c\u6307\u5357\u7684\u7bc4\u570d\u3002<\/strong> \u9019\u88e1\u63cf\u8ff0\u7684\u9a57\u8b49\u65b9\u6cd5\u4f86\u81ea\u5df2\u767c\u8868\u7684\u85e5\u5178\u898f\u7a0b\u3002\u6fc3\u5ea6\u3001\u5bb9\u5dee\u8207\u7a0b\u5e8f\u8207\u64b0\u5beb\u6642\u7684 USP <851> \u8207 EP 2.2.25 \u4e00\u81f4\u3002\u8981\u5f97\u5230\u5177\u7d04\u675f\u529b\u7684\u5408\u898f\u7b54\u6848\uff0c\u6c38\u9060\u53c3\u7167\u9069\u7528\u65bc\u4f60\u7ba1\u8f44\u5340\u7684\u7576\u524d\u5df2\u767c\u8868\u65b9\u6cd5\u7248\u672c\u3002\u672c\u9801\u662f\u5be6\u52d9\u8005\u53c3\u8003\u3001\u4e0d\u662f\u5b98\u65b9\u5c08\u8ad6\u7684\u66ff\u4ee3\u3002\n<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg1-t\"><title id=\"svg1-t\">\u4e94\u500b\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\u7dad\u5ea6\u8207\u4e09\u500b\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5\uff0c\u4ee5\u516b\u5f35\u5716\u793a\u5361\u6392\u6210\u683c\u72c0\uff0c\u6db5\u84cb\u6ce2\u9577\u6e96\u78ba\u5ea6\u3001\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u3001\u7dda\u6027\u3001\u96dc\u6563\u5149\u3001\u89e3\u6790\u5ea6\uff0c\u4ee5\u53ca\u6bd4\u8272\u76bf\u5149\u7a0b\u3001\u914d\u5c0d\u5339\u914d\u8207\u5e73\u6574\u5ea6<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u9a57\u8b49\u7dad\u5ea6 \u2014 \u5149\u8b5c\u5100 + \u6bd4\u8272\u76bf<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">5 \u500b\u5100\u5668\u7dad\u5ea6\uff08\u4e0a\u6392\uff09+ 3 \u500b\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5\uff08\u4e0b\u6392\uff09<\/text><g font-family=\"Arial,sans-serif\"><g><rect x=\"20\" y=\"60\" width=\"120\" height=\"120\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"80\" y=\"92\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udd2d<\/text><text x=\"80\" y=\"118\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#1a2a6c\">\u6ce2\u9577<\/text><text x=\"80\" y=\"134\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u6e96\u78ba\u5ea6<\/text><text x=\"80\" y=\"156\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u9225<\/text><text x=\"80\" y=\"168\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u00b11 nm \u7d2b\u5916<\/text><\/g><g><rect x=\"160\" y=\"60\" width=\"120\" height=\"120\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"220\" y=\"92\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udcd1<\/text><text x=\"220\" y=\"118\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#1a2a6c\">\u5438\u5149\u5ea6<\/text><text x=\"220\" y=\"134\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u6e96\u78ba\u5ea6<\/text><text x=\"220\" y=\"156\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">K\u2082Cr\u2082O\u2087<\/text><text x=\"220\" y=\"168\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u00b12% \u76f8\u5c0d<\/text><\/g><g><rect x=\"300\" y=\"60\" width=\"120\" height=\"120\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"360\" y=\"92\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udcca<\/text><text x=\"360\" y=\"118\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#1a2a6c\">\u5149\u5ea6<\/text><text x=\"360\" y=\"134\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u7dda\u6027<\/text><text x=\"360\" y=\"156\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">0.05\u20132.5 AU<\/text><text x=\"360\" y=\"168\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">r\u00b2 \u2265 0.999<\/text><\/g><g><rect x=\"440\" y=\"60\" width=\"120\" height=\"120\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"500\" y=\"92\" text-anchor=\"middle\" font-size=\"22\">\ud83c\udf1e<\/text><text x=\"500\" y=\"118\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#1a2a6c\">\u96dc\u6563\u5149<\/text><text x=\"500\" y=\"134\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u5728\u622a\u6b62 \u03bb<\/text><text x=\"500\" y=\"156\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">KCl @198 nm<\/text><text x=\"500\" y=\"168\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">A > 1.5 AU<\/text><\/g><g><rect x=\"580\" y=\"60\" width=\"120\" height=\"120\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"640\" y=\"92\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udd2c<\/text><text x=\"640\" y=\"118\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#1a2a6c\">\u89e3\u6790\u5ea6<\/text><text x=\"640\" y=\"134\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\/\u983b\u5bec<\/text><text x=\"640\" y=\"156\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u7532\u82ef\/\u5df1\u70f7<\/text><text x=\"640\" y=\"168\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">A269\/A266 \u2265 1.5<\/text><\/g><g><rect x=\"100\" y=\"200\" width=\"120\" height=\"120\" fill=\"#fef3c7\" stroke=\"#ca8a04\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"160\" y=\"232\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udcd0<\/text><text x=\"160\" y=\"258\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#854d0e\">\u6bd4\u8272\u76bf\u5149\u7a0b<\/text><text x=\"160\" y=\"274\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u5149\u7a0b<\/text><text x=\"160\" y=\"296\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u00b10.005 cm \u5bb9\u5dee<\/text><text x=\"160\" y=\"308\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">CoA + \u9a57\u8b49<\/text><\/g><g><rect x=\"300\" y=\"200\" width=\"120\" height=\"120\" fill=\"#fef3c7\" stroke=\"#ca8a04\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"360\" y=\"232\" text-anchor=\"middle\" font-size=\"22\">\u2696<\/text><text x=\"360\" y=\"258\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#854d0e\">\u914d\u5c0d\u5339\u914d<\/text><text x=\"360\" y=\"274\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">2 \u6bd4\u8272\u76bf\u7d44<\/text><text x=\"360\" y=\"296\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u0394A < 0.005<\/text><text x=\"360\" y=\"308\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u8de8\u5149\u8b5c<\/text><\/g><g><rect x=\"500\" y=\"200\" width=\"120\" height=\"120\" fill=\"#fef3c7\" stroke=\"#ca8a04\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"560\" y=\"232\" text-anchor=\"middle\" font-size=\"22\">\ud83d\udd0d<\/text><text x=\"560\" y=\"258\" text-anchor=\"middle\" font-size=\"12\" font-weight=\"700\" fill=\"#854d0e\">\u7a97\u7247\u5e73\u6574\u5ea6<\/text><text x=\"560\" y=\"274\" text-anchor=\"middle\" font-size=\"10\" fill=\"#1a1a2e\">\u8207\u6f54\u6de8\u5ea6<\/text><text x=\"560\" y=\"296\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">< \u03bb\/4 \u689d\u7d0b<\/text><text x=\"560\" y=\"308\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u76ee\u8996 + \u900f\u5c04<\/text><\/g><\/g><text x=\"80\" y=\"194\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#1a2a6c\">\u5149\u8b5c\u5100\u7dad\u5ea6\uff08USP <851>\uff09<\/text><text x=\"360\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\" fill=\"#854d0e\">\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5<\/text><\/svg><figcaption>\u5716 1 \u2014 \u516b\u500b\u9a57\u8b49\u7dad\u5ea6\u3002\u4e0a\u6392\uff1aUSP <851>\u3001EP 2.2.25 \u8207\u7b49\u6548\u85e5\u5178\u898f\u5b9a\u7684\u4e94\u500b\u5100\u5668\u9a57\u8b49\u3002\u4e0b\u6392\uff1a\u5b8c\u6210\u53d7\u898f\u7bc4\u5be6\u9a57\u5ba4\u78ba\u6548\u5305\u7684\u4e09\u500b\u6bd4\u8272\u76bf\u7279\u6709\u6aa2\u67e5\u2014\u2014\u6c92\u6709\u9019\u4e9b\uff0c\u5149\u8b5c\u5100\u53ef\u4ee5\u5b8c\u7f8e\u7121\u7455\u3001\u800c\u4f60\u7684\u6578\u64da\u4ecd\u7ad9\u4e0d\u4f4f\u8173\u3002<\/figcaption><\/figure><h2 id=\"why\">1. \u70ba\u4f55\u9a57\u8b49\u91cd\u8981 \u2014 \u7a3d\u6838\u54e1\u770b\u4ec0\u9ebc<\/h2>\n<p>\u5be9\u4f60\u5206\u5149\u5149\u5ea6\u8a08 SOP \u7684 FDA\u3001EMA \u6216\u7576\u5730\u6cd5\u898f\u7a3d\u67e5\u54e1\uff0c\u6703\u4f9d\u5e8f\u6aa2\u67e5\u4e09\u4ef6\u4e8b\u3002\u7b2c\u4e00\uff0cSOP \u5b58\u5728\u4e14\u53c3\u7167\u9069\u7528\u7684\u85e5\u5178\u65b9\u6cd5\uff08\u4f9d\u7ba1\u8f44\u5340\u662f USP <851>\u3001EP 2.2.25\u3001JP 2.24 \u6216 ChP 0401\uff09\u3002\u7b2c\u4e8c\uff0c\u9a57\u8b49\u78ba\u5be6\u4ee5\u6587\u4ef6\u8a18\u8f09\u7684\u983b\u7387\u57f7\u884c\u4e86\u3002\u7b2c\u4e09\uff0c\u7d00\u9304\u6709\u7c3d\u540d\u3001\u65e5\u671f\u3001\u53ef\u8ffd\u6eaf\u5230\u6821\u6b63\u904e\u7684\u53c3\u8003\u6a19\u6e96\uff0c\u4e26\u7531\u54c1\u8cea\u5be9\u6838\u3002\u9019\u4e09\u6b65\u4efb\u4e00\u6709\u7f3a\u53e3\uff0c\u5c31\u662f\u4e00\u689d\u53ef\u958b\u7acb\u7684\u7f3a\u5931\u3002<\/p>\n<h3>\u6c92\u6709\u9a57\u8b49\u6703\u51fa\u4ec0\u9ebc\u932f<\/h3>\n<ul>\n<li><strong>\u71c8\u6f02\u79fb<\/strong> \u2014\u2014\u6c18\u71c8\u8207\u93a2\u71c8\u5728\u516d\u500b\u6708\u7684\u7dad\u4fee\u9031\u671f\u5167\u628a\u8868\u89c0\u6ce2\u9577\u4f4d\u79fb 0.1\u20130.3 nm\u3002\u4e00\u500b\u6b63\u597d\u5728 280 nm \u6821\u6b63\u7684\u65b9\u6cd5\u73fe\u5728\u8b80 280.2 nm\uff1b\u5f85\u6e2c\u7269\u5728 280.2 \u7684\u83ab\u8033\u5438\u5149\u4fc2\u6578\u8207\u5b83\u5728 280.0 \u7684\u503c\u4e0d\u540c\uff0c\u4f9d\u5438\u6536\u5cf0\u591a\u9661\u800c\u628a\u7d50\u679c\u504f\u5dee 1\u20133%\u3002<\/li>\n<li><strong>\u5075\u6e2c\u5668\u8001\u5316<\/strong> \u2014\u2014\u5149\u96fb\u500d\u589e\u7ba1\u8207\u77fd\u5149\u4e8c\u6975\u9ad4\u5075\u6e2c\u5668\u5728\u9ad8\u5438\u5149\u5ea6\u6bcf\u5e74\u640d\u5931 0.5\u20131% \u7684\u5149\u5ea6\u6e96\u78ba\u5ea6\u3002\u6c92\u6709\u9031\u671f\u6027\u7684 K\u2082Cr\u2082O\u2087 \u9a57\u8b49\uff0c\u9019\u7a2e\u8001\u5316\u770b\u4e0d\u898b\uff0c\u76f4\u5230\u4f60\u62ff\u4e00\u53f0\u65b0\u5100\u5668\u4f86\u6bd4\u3002<\/li>\n<li><strong>\u6bd4\u8272\u76bf\u78e8\u640d<\/strong> \u2014\u2014\u5373\u4f7f\u662f\u7194\u878d\u77f3\u82f1\u7a97\u7247\uff0c\u6578\u6708\u4f7f\u7528\u4e0b\u4f86\u4e5f\u6703\u6cbe\u4e0a\u7d30\u5fae\u522e\u75d5\u3001\u8584\u819c\u8207\u6c59\u67d3\u3002\u914d\u5c0d\u7684\u6bd4\u8272\u76bf\u96a8\u6642\u9593\u6f02\u958b\uff0c\u56e0\u70ba\u5176\u4e2d\u4e00\u652f\u7d2f\u7a4d\u7684\u78e8\u640d\u6bd4\u53e6\u4e00\u652f\u591a\u3002<\/li>\n<li><strong>\u96dc\u6563\u5149\u589e\u52a0<\/strong> \u2014\u2014\u55ae\u8272\u5668\u8207\u5149\u675f\u8655\u7406\u5149\u5b78\u7684\u9000\u5316\u62ac\u9ad8\u96dc\u6563\u5149\uff0c\u5728\u4efb\u4f55\u5f85\u6e2c\u7269\u7684\u9577\u6ce2\u9577\u5438\u6536\u908a\u7de3\uff08\u90a3\u88e1\u771f\u5be6\u8a0a\u865f\u5f88\u5c0f\uff09\u504f\u5dee\u91cf\u6e2c\u3002<\/li>\n<\/ul>\n\n<div class=\"csg-callout warn\"><strong>\u6211\u5011\u5728\u5ba2\u6236\u7a3d\u67e5\u88e1\u770b\u904e\u7684\u53ef\u958b\u7acb\u7f3a\u5931\u3002<\/strong> \u6700\u5e38\u898b\u7684\u767c\u73fe\u662f\uff1a(1) \u9a57\u8b49 SOP \u5b58\u5728\u3001\u4f46\u76f8\u5c0d\u65bc\u7576\u524d\u85e5\u5178\u65b9\u6cd5\u904e\u671f\u8d85\u904e\u4e00\u5e74\uff1b(2) \u9a57\u8b49\u7d00\u9304\u986f\u793a\u4e00\u5e74\u7684\u6bcf\u6708\u6aa2\u67e5\u3001\u7136\u5f8c\u4e00\u500b 4 \u500b\u6708\u7684\u7f3a\u53e3\uff1b(3) \u5b58\u6a94\u7684\u6bd4\u8272\u76bf CoA \u662f\u539f\u59cb\u63a1\u8cfc\u6587\u4ef6\u3001\u7528\u4e86\u597d\u5e7e\u5e74\u5f9e\u672a\u91cd\u65b0\u9a57\u8b49\uff1b(4) \u53c3\u8003\u9225\u6eb6\u6db2\u904e\u4e86 18 \u500b\u6708\u7684\u6709\u6548\u671f\u3002\u6bcf\u4e00\u500b\u90fd\u80fd\u7528\u5169\u5c0f\u6642\u7684 SOP \u5de5\u4f5c\u4fee\u597d\u3002<\/div><h2 id=\"five\">2. \u4e94\u500b\u5149\u8b5c\u5100\u9a57\u8b49\u7dad\u5ea6<\/h2>\n<p>USP <851>\u3001EP 2.2.25\u3001JP 2.24 \u8207 ChP 0401 \u5728\u4e94\u500b\u7dad\u5ea6\u4e0a\u672c\u8cea\u4e0a\u662f\u5354\u8abf\u4e00\u81f4\u7684\u3002\u53c3\u8003\u7269\u8cea\u3001\u76ee\u6a19\u5bb9\u5dee\u8207\u6aa2\u67e5\u983b\u7387\u5728\u7d30\u7bc0\u4e0a\u4e0d\u540c\u3001\u4f46\u6846\u67b6\u76f8\u540c\u3002<\/p>\n<div class=\"csg-dim-grid\">\n<div class=\"csg-dim-card\"><span class=\"csg-dim-icon\">\ud83d\udd2d<\/span><h4>1. \u6ce2\u9577\u6e96\u78ba\u5ea6<\/h4><p>\u8868\u89c0 \u03bb = \u5be6\u969b \u03bb \u00b1 \u5bb9\u5dee\u3002\u7528\u9225\u6c27\u5316\u7269\u6eb6\u6db2\uff08USP\uff09\u6216\u9225\u73bb\u7483\uff08\u5feb\u67e5\uff09\u9a57\u8b49\u3002<\/p><\/div>\n<div class=\"csg-dim-card\"><span class=\"csg-dim-icon\">\ud83d\udcd1<\/span><h4>2. \u5438\u5149\u5ea6\u6e96\u78ba\u5ea6<\/h4><p>\u5728\u591a\u500b\u5438\u5149\u5ea6\u503c\u4e0a\uff0c\u91cf\u5230\u7684 AU = \u8a8d\u8b49 AU \u00b1 \u5bb9\u5dee\u30025 mM H\u2082SO\u2084 \u4e2d\u7684 K\u2082Cr\u2082O\u2087 \u662f USP \u53c3\u8003\u3002<\/p><\/div>\n<div class=\"csg-dim-card\"><span class=\"csg-dim-icon\">\ud83d\udcca<\/span><h4>3. \u5149\u5ea6\u7dda\u6027<\/h4><p>\u5075\u6e2c\u5668\u97ff\u61c9\u5728 0.05 \u5230 ~2.5 AU \u7dda\u6027\u3002\u7528 K\u2082Cr\u2082O\u2087 \u7a00\u91cb\u7cfb\u5217\u6e2c\u8a66\uff1br\u00b2 \u2265 0.999\u3002<\/p><\/div>\n<div class=\"csg-dim-card\"><span class=\"csg-dim-icon\">\ud83c\udf1e<\/span><h4>4. \u96dc\u6563\u5149<\/h4><p>\u5728\u6240\u9078\u983b\u5bec\u4e4b\u5916\u5230\u9054\u5075\u6e2c\u5668\u7684\u5149\u3002\u7528\u5f37\u622a\u6b62\u6ffe\u5149\u7247\u6e2c\u8a66\uff081.2% KCl @198 nm\u30011% NaI @220 nm\uff09\u3002<\/p><\/div>\n<div class=\"csg-dim-card\"><span class=\"csg-dim-icon\">\ud83d\udd2c<\/span><h4>5. \u89e3\u6790\u5ea6 \/ \u983b\u5bec<\/h4><p>\u55ae\u8272\u5668\u7684\u5149\u8b5c\u983b\u5bec\uff08SBW\uff09\u3002\u7528 0.020% v\/v \u5df1\u70f7\u4e2d\u7532\u82ef\u6e2c\u8a66\u2014\u2014A269\/A266 \u6bd4\u503c \u2265 1.5\uff08USP \u76ee\u6a19\uff09\u3002<\/p><\/div>\n<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg2-t\"><title id=\"svg2-t\">\u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2 200 \u5230 700 \u5948\u7c73\u7684 UV-\u53ef\u898b\u5438\u6536\u5149\u8b5c\uff0c\u986f\u793a\u4f9d USP 851 \u7528\u65bc\u5206\u5149\u5149\u5ea6\u8a08\u6ce2\u9577\u6821\u6b63\u7684\u7279\u5fb5\u7a84\u5438\u6536\u5cf0<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u9225\u6c27\u5316\u7269\u6eb6\u6db2 \u2014 \u6ce2\u9577\u6821\u6b63\u5149\u8b5c<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">\u4f9d USP <851> 1.4 M HClO\u2084 \u4e2d 4% Ho\u2082O\u2083\uff1b\u4f9b \u03bb \u6e96\u78ba\u5ea6\u9a57\u8b49\u7684\u7279\u5fb5\u5cf0<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"240\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"100\" x2=\"680\" y2=\"100\"\/><line x1=\"80\" y1=\"160\" x2=\"680\" y2=\"160\"\/><line x1=\"80\" y1=\"220\" x2=\"680\" y2=\"220\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"300\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"300\"\/><\/g><line x1=\"80\" y1=\"300\" x2=\"680\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"316\" text-anchor=\"middle\">200<\/text><text x=\"200\" y=\"316\" text-anchor=\"middle\">300<\/text><text x=\"320\" y=\"316\" text-anchor=\"middle\">400<\/text><text x=\"440\" y=\"316\" text-anchor=\"middle\">500<\/text><text x=\"560\" y=\"316\" text-anchor=\"middle\">600<\/text><text x=\"680\" y=\"316\" text-anchor=\"middle\">700<\/text><\/g><text x=\"380\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u6ce2\u9577\uff08nm\uff09<\/text><text x=\"36\" y=\"180\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 180)\">\u5438\u5149\u5ea6\uff08AU\uff09<\/text><path d=\"M 80 290 L 130 285 L 145 230 L 155 285 L 180 280 L 195 200 L 205 285 L 230 280 L 250 250 L 260 235 L 275 285 L 290 280 L 320 270 L 340 195 L 350 280 L 380 270 L 415 240 L 425 270 L 445 240 L 455 270 L 480 270 L 500 240 L 510 280 L 545 230 L 555 280 L 645 270 L 655 200 L 670 280 L 680 280\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" fill=\"none\"\/><g font-family=\"Arial,sans-serif\" font-size=\"9\" font-weight=\"700\" fill=\"#dc2626\"><line x1=\"149\" y1=\"225\" x2=\"149\" y2=\"218\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"149\" y=\"212\" text-anchor=\"middle\">241.13<\/text><line x1=\"200\" y1=\"195\" x2=\"200\" y2=\"188\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"200\" y=\"182\" text-anchor=\"middle\">287.18<\/text><line x1=\"256\" y1=\"230\" x2=\"256\" y2=\"223\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"256\" y=\"217\" text-anchor=\"middle\">345.47<\/text><line x1=\"346\" y1=\"190\" x2=\"346\" y2=\"183\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"346\" y=\"177\" text-anchor=\"middle\">416.28<\/text><line x1=\"425\" y1=\"235\" x2=\"425\" y2=\"228\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"425\" y=\"222\" text-anchor=\"middle\">451.30<\/text><line x1=\"455\" y1=\"235\" x2=\"455\" y2=\"228\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"455\" y=\"244\" text-anchor=\"middle\">467.83<\/text><line x1=\"505\" y1=\"235\" x2=\"505\" y2=\"228\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"505\" y=\"222\" text-anchor=\"middle\">485.29<\/text><line x1=\"551\" y1=\"225\" x2=\"551\" y2=\"218\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"551\" y=\"212\" text-anchor=\"middle\">536.64<\/text><line x1=\"654\" y1=\"195\" x2=\"654\" y2=\"188\" stroke=\"#dc2626\" stroke-width=\"1\"\/><text x=\"654\" y=\"182\" text-anchor=\"middle\">640.49<\/text><\/g><\/svg><figcaption>\u5716 2 \u2014 \u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2 UV-Vis \u5149\u8b5c\uff08USP <851> \u53c3\u8003\uff09\u3002\u5341\u4e09\u500b\u7279\u5fb5\u5438\u6536\u5cf0\u6a6b\u8de8 241 \u5230 640 nm\uff0c\u6db5\u84cb\u7d2b\u5916\u5230\u53ef\u898b\u3002\u8868\u89c0\u5cf0\u4f4d\u7f6e\u8207\u8a8d\u8b49\u503c\u6bd4\u8f03\uff1b\u5bb9\u5dee\u901a\u5e38\u5728\u7d2b\u5916\uff08400 nm \u4ee5\u4e0b\uff09\u00b11 nm\u3001\u5728\u53ef\u898b\uff08400 nm \u4ee5\u4e0a\uff09\u00b13 nm\u3002\u5cf0\u4f4d\u7f6e\u7a69\u5b9a\u3001\u7a84\u3001\u4e14\u5206\u8fa8\u826f\u597d\uff0c\u4f7f\u9019\u6210\u70ba\u6ce2\u9577\u6821\u6b63\u7684\u9ec3\u91d1\u6a19\u6e96\u7269\u8cea\u3002<\/figcaption><\/figure><h2 id=\"wavelength\">3. \u6ce2\u9577\u6e96\u78ba\u5ea6 \u2014 \u9225\u6a19\u6e96<\/h2>\n<p>\u6ce2\u9577\u6e96\u78ba\u5ea6\u9a57\u8b49\u56de\u7b54\u4e00\u500b\u554f\u984c\uff1a\u7576\u5149\u8b5c\u5100\u55ae\u8272\u5668\u8a2d\u5728 280 nm \u6642\uff0c\u5be6\u969b\u900f\u5c04\u7684\u6ce2\u9577\u662f 280.0 nm \u55ce\uff1f\u85e5\u5178\u63a5\u53d7\u4e09\u7a2e\u53c3\u8003\u7269\u8cea\u3002<\/p>\n\n<h3>\u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2\uff08USP <851> \u4e3b\u8981\uff09<\/h3>\n<p>4% w\/v \u9225\u6c27\u5316\u7269\uff08Ho\u2082O\u2083\uff09\u6eb6\u65bc 1.4 M \u904e\u6c2f\u9178\uff08HClO\u2084\uff09\uff0c\u586b\u5165\u5e36\u585e\u7684 10 mm \u77f3\u82f1\u6bd4\u8272\u76bf\u3002\u5341\u4e09\u500b\u7279\u5fb5\u5438\u6536\u5cf0\u5728 241.13\u3001249.79\u3001278.10\u3001287.18\u3001333.44\u3001345.47\u3001361.31\u3001416.28\u3001451.30\u3001467.83\u3001485.29\u3001536.64 \u8207 640.49 nm\uff0c\u6db5\u84cb\u7d2b\u5916\u5230\u53ef\u898b\u3002\u8a8d\u8b49\u5cf0\u4f4d\u7f6e\u900f\u904e SRM 2034 \u53ef\u8ffd\u6eaf\u5230 NIST\u3002<\/p>\n<ul>\n<li><strong>\u5bb9\u5dee\uff1a<\/strong> \u03bb \u2264 400 nm \u6642 \u00b11 nm\uff1b\u03bb > 400 nm \u6642 \u00b13 nm\uff08USP <851>\uff09<\/li>\n<li><strong>\u4f7f\u7528\u58fd\u547d\uff1a<\/strong> \u5728\u539f\u6bd4\u8272\u76bf\u88e1\u65bc 15\u201330 \u00b0C \u5bc6\u5c01\u4fdd\u5b58\u5247 5 \u5e74<\/li>\n<li><strong>\u53d6\u5f97\uff1a<\/strong> NIST SRM 2034\u3001Starna Cells\u300c\u9225\u53c3\u8003\u6eb6\u6db2\u300d\uff0c\u6216\u4f9d USP \u65b9\u6cd5\u81ea\u884c\u88fd\u5099<\/li>\n<\/ul>\n\n<h3>\u9225\u73bb\u7483\uff08\u56fa\u9ad4\u53c3\u8003\uff09<\/h3>\n<p>\u4e00\u584a\u647b\u9225\u73bb\u7483\u7684\u56fa\u9ad4\u584a\uff0c\u5207\u6210\u6a19\u6e96 12.5 \u00d7 12.5 \u00d7 55 mm \u6bd4\u8272\u76bf\u5916\u5c3a\u5bf8\u3002\u8207\u6eb6\u6db2\u76f8\u540c\u7684\u7279\u5fb5\u5cf0\uff0c\u88ab\u56fa\u9ad4\u57fa\u8cea\u7565\u5fae\u5c55\u5bec\u3002\u5c0d\u4f8b\u884c\u5feb\u67e5\u65b9\u4fbf\uff0c\u56e0\u70ba\u6c92\u6709\u6771\u897f\u5728\u6eb6\u6db2\u88e1\uff0c\u8a8d\u8b49\u5cf0\u4f4d\u7f6e\u5728\u73bb\u7483\u7684\u58fd\u547d\uff0810+ \u5e74\uff09\u5167\u7a69\u5b9a\u3002<\/p>\n<ul>\n<li><strong>\u63a5\u53d7\u5ea6\uff1a<\/strong> USP \u51c6\u8a31\u7528\u65bc\u4f8b\u884c\u7684\u4e2d\u9593\u6aa2\u67e5\uff1b\u6db2\u9ad4\u6eb6\u6db2\u662f\u5b8c\u6574\u78ba\u6548\u7684\u53c3\u8003<\/li>\n<li><strong>\u6ce2\u9577\u7bc4\u570d\uff1a<\/strong> 240\u2013640 nm\uff08\u50c5 UV-Vis\uff1b\u66f4\u6df1\u7684\u7d2b\u5916\u9700\u8981\u6eb6\u6db2\uff09<\/li>\n<li><strong>\u5bb9\u5dee\uff1a<\/strong> \u5cf0\u4f4d\u7f6e\u6bd4\u6eb6\u6db2\u7565\u5bec\uff1b\u7528\u73bb\u7483\u88fd\u9020\u5546\u63d0\u4f9b\u7684\u8a8d\u8b49\u5149\u8b5c\u9a57\u8b49<\/li>\n<\/ul>\n\n<h3>\u91f9\u9420\u73bb\u7483\uff08\u8f03\u5bec\u7bc4\u570d\u7684\u66ff\u4ee3\uff09<\/h3>\n<p>\u91f9\u9420 = \u647b\u91f9 + \u9420\u6c27\u5316\u7269\u7684\u73bb\u7483\u3002\u6db5\u84cb\u8f03\u5bec\u7684\u6ce2\u9577\u7bc4\u570d\uff08\u901a\u5e38 190\u2013900 nm\uff09\uff0c\u5cf0\u5728 431\u3001522\u3001580\u3001685\u3001745 \u8207 803 nm\uff0c\u5728\u9225\u5cf0\u8f03\u5c11\u7684\u53ef\u898b-\u8fd1\u7d05\u5916\u5340\u6709\u7528\u3002<\/p>\n\n<h3>\u6c5e\u71c8\u767c\u5c04\u8b5c\u7dda\uff08\u8f03\u820a\u7684\u65b9\u6cd5\uff09<\/h3>\n<p>\u67d0\u4e9b\u8f03\u820a\u7684\u5206\u5149\u5149\u5ea6\u8a08\u8a2d\u8a08\u542b\u4f4e\u58d3\u6c5e\u5f27\u71c8\u914d\u4ef6\u3002Hg \u5728 253.65\u3001365.02\u3001404.66\u3001435.83\u3001546.07 \u8207 578.97 nm \u7684\u767c\u5c04\u8b5c\u7dda\u63d0\u4f9b\u7d55\u5c0d\u6ce2\u9577\u53c3\u8003\u3002\u5728\u73fe\u4ee3\u5100\u5668\u88e1\u8f03\u5c11\u898b\u3001\u4f46 USP \u8207 EP \u4ecd\u63a5\u53d7\u3002<\/p>\n\n<div class=\"csg-callout protocol\"><strong>\u9a57\u8b49\u898f\u7a0b\uff08\u5178\u578b\uff09\u3002<\/strong> \u628a\u9225\u6bd4\u8272\u76bf\u63d2\u5165\u6a23\u54c1\u5ba4\u3002\u4ee5\u5be6\u52d9\u4e0a\u6700\u6162\u7684\u901f\u5ea6\u8207\u6700\u7a84\u7684\u5be6\u52d9\u983b\u5bec\uff08\u901a\u5e38 1 nm \u6216\u66f4\u5c0f\uff09\u5f9e 240 \u6383\u5230 650 nm\u3002\u8fa8\u8b58\u6bcf\u500b\u7279\u5fb5\u5cf0\u3002\u8a18\u9304\u6bcf\u500b\u7684\u8868\u89c0\u6ce2\u9577\u3002\u8207\u8a8d\u8b49\u503c\u6bd4\u8f03\u3002\u7b97\u51fa\u5dee\u503c\u3002\u4f9d\u4e0a\u9762\u7684\u5bb9\u5dee\u8868\u901a\u904e\/\u4e0d\u901a\u904e\u3002\u5728\u7d00\u9304\u4e0a\u7c3d\u540d\u8207\u8a3b\u660e\u65e5\u671f\u3002<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 360\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg3-t\"><title id=\"svg3-t\">\u91cd\u927b\u9178\u9240\u5728\u56db\u500b\u6ce2\u9577\u7684\u6821\u6b63\u66f2\u7dda\uff0c\u986f\u793a\u5438\u5149\u5ea6\u5c0d\u6fc3\u5ea6\uff0c\u4f9b\u4f9d USP 851 \u9a57\u8b49\u5206\u5149\u5149\u5ea6\u8a08\u5149\u5ea6\u6e96\u78ba\u5ea6\u8207\u7dda\u6027<\/title><rect width=\"720\" height=\"360\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">K\u2082Cr\u2082O\u2087 \u53c3\u8003 \u2014 \u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u8207\u7dda\u6027<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">USP <851> 5 mM H\u2082SO\u2084 \u4e2d\u7684 SRM 935a \u2014 \u56db\u500b\u6ce2\u9577\u7684 A\u00b9% \u503c<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"240\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"100\" x2=\"680\" y2=\"100\"\/><line x1=\"80\" y1=\"160\" x2=\"680\" y2=\"160\"\/><line x1=\"80\" y1=\"220\" x2=\"680\" y2=\"220\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"300\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"300\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"300\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"300\"\/><\/g><line x1=\"80\" y1=\"300\" x2=\"680\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"300\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"316\" text-anchor=\"middle\">0<\/text><text x=\"200\" y=\"316\" text-anchor=\"middle\">25<\/text><text x=\"320\" y=\"316\" text-anchor=\"middle\">50<\/text><text x=\"440\" y=\"316\" text-anchor=\"middle\">75<\/text><text x=\"560\" y=\"316\" text-anchor=\"middle\">100<\/text><text x=\"680\" y=\"316\" text-anchor=\"middle\">125<\/text><\/g><text x=\"380\" y=\"334\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">K\u2082Cr\u2082O\u2087 \u6fc3\u5ea6\uff08mg\/L\uff09<\/text><text x=\"36\" y=\"180\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 180)\">\u5438\u5149\u5ea6\uff08AU\u300110 mm \u6bd4\u8272\u76bf\uff09<\/text><line x1=\"80\" y1=\"290\" x2=\"680\" y2=\"80\" stroke=\"#dc2626\" stroke-width=\"2.4\"\/><line x1=\"80\" y1=\"295\" x2=\"680\" y2=\"120\" stroke=\"#1a2a6c\" stroke-width=\"2.4\"\/><line x1=\"80\" y1=\"297\" x2=\"680\" y2=\"190\" stroke=\"#0ea5e9\" stroke-width=\"2.4\"\/><line x1=\"80\" y1=\"299\" x2=\"680\" y2=\"240\" stroke=\"#16a34a\" stroke-width=\"2.4\"\/><g><circle cx=\"80\" cy=\"290\" r=\"4\" fill=\"#dc2626\"\/><circle cx=\"200\" cy=\"248\" r=\"4\" fill=\"#dc2626\"\/><circle cx=\"320\" cy=\"206\" r=\"4\" fill=\"#dc2626\"\/><circle cx=\"440\" cy=\"164\" r=\"4\" fill=\"#dc2626\"\/><circle cx=\"560\" cy=\"122\" r=\"4\" fill=\"#dc2626\"\/><circle cx=\"80\" cy=\"295\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"200\" cy=\"260\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"320\" cy=\"225\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"440\" cy=\"190\" r=\"4\" fill=\"#1a2a6c\"\/><circle cx=\"560\" cy=\"155\" r=\"4\" fill=\"#1a2a6c\"\/><\/g><g font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"700\"><text x=\"690\" y=\"84\" text-anchor=\"end\" fill=\"#dc2626\">235 nm\uff08A\u00b9%=124.5\uff09<\/text><text x=\"690\" y=\"124\" text-anchor=\"end\" fill=\"#1a2a6c\">257 nm\uff08A\u00b9%=144\uff09<\/text><text x=\"690\" y=\"194\" text-anchor=\"end\" fill=\"#0369a1\">313 nm\uff08A\u00b9%=48.6\uff09<\/text><text x=\"690\" y=\"244\" text-anchor=\"end\" fill=\"#15803d\">350 nm\uff08A\u00b9%=106.6\uff09<\/text><\/g><\/svg><figcaption>\u5716 3 \u2014 \u91cd\u927b\u9178\u9240\u5728\u56db\u500b USP \u8a8d\u8b49\u6ce2\u9577\u7684\u6821\u6b63\u66f2\u7dda\u3002\u6bcf\u689d\u7dda\u662f 10 mm \u6bd4\u8272\u76bf\u88e1\u5438\u5149\u5ea6\u5c0d\u6fc3\u5ea6\u7684\u6717\u4f2f-\u6bd4\u723e\u5716\u3002\u6bcf\u500b\u6ce2\u9577\u7684\u659c\u7387\u7d66\u51fa\u5df2\u767c\u8868\u7684 A\u00b9%\uff081% w\/v \u6eb6\u6db2\u7684\u5438\u5149\u4fc2\u6578\uff09\uff1b\u91cf\u5230\u7684\u659c\u7387\u5728\u8a8d\u8b49\u503c\u7684 \u00b12% \u5167\u5c31\u901a\u904e\u5149\u5ea6\u6e96\u78ba\u5ea6\u3002\u7dda\u6027\uff08\u76f8\u95dc\u4fc2\u6578 r\u00b2 \u2265 0.999\uff09\u78ba\u8a8d\u5075\u6e2c\u5668\u5728 0.1\u20131.5 AU \u7684\u7dda\u6027\u3002<\/figcaption><\/figure><h2 id=\"absorbance\">4. \u5438\u5149\u5ea6\u6e96\u78ba\u5ea6 \u2014 \u56db\u500b\u6ce2\u9577\u7684\u91cd\u927b\u9178\u9240<\/h2>\n<p>\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u9a57\u8b49\u56de\u7b54\uff1a\u7576\u5100\u5668\u8b80 1.000 AU \u6642\uff0c\u771f\u5be6\u5438\u5149\u5ea6\u662f 1.000 AU \u55ce\uff1f\u85e5\u5178\u53c3\u8003\u662f 5 mM\uff080.005 M\uff09\u786b\u9178\u4e2d\u7684\u91cd\u927b\u9178\u9240\uff08K\u2082Cr\u2082O\u2087\uff09\u2014\u2014\u5177\u9ad4\u662f NIST SRM 935a\uff0c\u4e00\u7a2e\u9ad8\u7d14\u5ea6\u8a8d\u8b49\u7269\u8cea\u3002<\/p>\n\n<h3>USP <851> \u5df2\u767c\u8868\u503c<\/h3>\n<p>5 mM H\u2082SO\u2084 \u4e2d\u7684 K\u2082Cr\u2082O\u2087 \u6709\u5df2\u77e5\u7684\u5438\u5149\u4fc2\u6578\u503c\uff08A\u00b9%\uff0c\u5373 1% w\/v \u6eb6\u6db2\u5728 10 mm \u6bd4\u8272\u76bf\u88e1\u7684\u5438\u5149\u5ea6\uff09\uff1a<\/p>\n<table class=\"csg-protocol\">\n<thead><tr><th>\u6ce2\u9577<\/th><th>A\u00b9% \u8a8d\u8b49\u503c<\/th><th>\u5bb9\u5dee<\/th><th>\u7528\u9014<\/th><\/tr><\/thead>\n<tbody>\n<tr><td>235 nm<\/td><td>124.5<\/td><td>\u00b12% \u76f8\u5c0d<\/td><td>\u9060\u7d2b\u5916\uff1b\u71c8\u6cc1<\/td><\/tr>\n<tr><td>257 nm<\/td><td>144.0<\/td><td>\u00b12% \u76f8\u5c0d<\/td><td>\u4e2d\u7d2b\u5916\uff1b\u4e3b\u8981\u6aa2\u67e5<\/td><\/tr>\n<tr><td>313 nm<\/td><td>48.6<\/td><td>\u00b12% \u76f8\u5c0d<\/td><td>\u8f03\u4f4e AU \u7a97\u53e3<\/td><\/tr>\n<tr><td>350 nm<\/td><td>106.6<\/td><td>\u00b12% \u76f8\u5c0d<\/td><td>\u53ef\u898b\u908a\u7de3\u6aa2\u67e5<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<h3>\u9a57\u8b49\u898f\u7a0b<\/h3>\n<ol>\n<li>\u5f9e SRM 935a \u6216\u7b49\u6548\u7684 NIST \u53ef\u8ffd\u6eaf\u7269\u8cea\uff0c\u5728 5 mM H\u2082SO\u2084 \u4e2d\u88fd\u5099 25\u300150\u300175 \u8207 100 mg\/L \u7684 K\u2082Cr\u2082O\u2087 \u6eb6\u6db2\u3002<\/li>\n<li>\u5728\u55ae\u4e00\u914d\u5c0d\u7684 10 mm \u6bd4\u8272\u76bf\u88e1\uff0c\u5c0d 5 mM H\u2082SO\u2084 \u7a7a\u767d\uff0c\u5728 235\u3001257\u3001313 \u8207 350 nm \u8b80\u6bcf\u500b\u6eb6\u6db2\u3002<\/li>\n<li>\u6bcf\u500b\u6ce2\u9577\uff0c\u4f5c A \u5c0d\u6fc3\u5ea6\u5716\u3002\u7b97\u659c\u7387 = \u5be6\u9a57 A\u00b9%\u3002<\/li>\n<li>\u628a\u5be6\u9a57 A\u00b9% \u8207\u8a8d\u8b49\u503c\u6bd4\u8f03\u3002\u5728 \u00b12% \u76f8\u5c0d\u5167\u5c31\u901a\u904e\u3002<\/li>\n<\/ol>\n\n<div class=\"csg-callout\"><strong>\u70ba\u4f55\u5169\u7a2e\u65b9\u6cd5\uff08\u9225\u300c\u548c\u300d\u91cd\u927b\u9178\u9e7d\uff09\u3002<\/strong> \u9225\u9a57\u8b49\u6ce2\u9577\u6e96\u78ba\u5ea6\u2014\u2014\u5149\u8b5c\u5100\u6b63\u78ba\u5730\u8b80\u6ce2\u9577\u523b\u5ea6\u3002K\u2082Cr\u2082O\u2087 \u9a57\u8b49\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u2014\u2014\u5149\u8b5c\u5100\u6b63\u78ba\u5730\u8b80\u5438\u5149\u5ea6\u523b\u5ea6\u3002\u5169\u8005\u90fd\u5fc5\u9808\u901a\u904e\uff1b\u901a\u904e\u5176\u4e00\u4e0d\u78ba\u6548\u53e6\u4e00\u3002\u5169\u8005\u90fd\u662f\u7b26\u5408 USP <851> \u7684\u5100\u5668\u6240\u5fc5\u9700\u3002<\/div><h2 id=\"linearity\">5. \u5149\u5ea6\u7dda\u6027 \u2014 \u7a00\u91cb\u7cfb\u5217<\/h2>\n<p>\u7dda\u6027\u9a57\u8b49\u78ba\u8a8d\u5075\u6e2c\u5668\u5728\u5de5\u4f5c\u7bc4\u570d\u5167\u5c0d\u5438\u5149\u5ea6\u7dda\u6027\u97ff\u61c9\u30021.5\u20132.0 AU \u4ee5\u4e0a\uff0c\u6240\u6709\u5075\u6e2c\u5668\u90fd\u56e0\u9ad8\u5149\u5bc6\u5ea6\u4e0b\u6717\u4f2f-\u6bd4\u723e\u5931\u6548\u3001\u4ee5\u53ca\u5075\u6e2c\u5668\u96dc\u8a0a\u4e3b\u5bb0\u4f4e\u900f\u5c04\u5f37\u5ea6\u800c\u504f\u96e2\u7dda\u6027\u3002<\/p>\n<h3>\u65b9\u6cd5<\/h3>\n<p>\u7528 K\u2082Cr\u2082O\u2087 \u53c3\u8003\uff0c\u88fd\u5099\u5f9e 5 \u5230 100 mg\/L \u7684 6\u20138 \u500b\u6fc3\u5ea6\u7684\u7a00\u91cb\u7cfb\u5217\uff08\u6db5\u84cb 257 nm \u8655 A \u5f9e 0.05 \u5230 ~1.5 AU\uff09\u3002\u91cf\u6bcf\u500b\u6eb6\u6db2\u7684\u5438\u5149\u5ea6\u3002\u4f5c A \u5c0d\u6fc3\u5ea6\u5716\u3002\u8a08\u7b97\uff1a<\/p>\n<ul>\n<li><strong>\u7dda\u6027\uff08r\u00b2\uff09\uff1a<\/strong> \u5728 0.1\u20131.5 AU \u7bc4\u570d\u5167 \u2265 0.999\u3002\u67d0\u4e9b\u85e5\u5178\u63a5\u53d7 r\u00b2 \u2265 0.998\u3002<\/li>\n<li><strong>\u659c\u7387\uff1a<\/strong> 257 nm \u7684\u659c\u7387\u61c9\u5728 \u00b12% \u76f8\u5c0d\u5167\u7b26\u5408 A\u00b9% = 144\u3002<\/li>\n<li><strong>\u622a\u8ddd\uff1a<\/strong> \u5728\u5be6\u9a57\u96dc\u8a0a\u5167\u61c9\u8207\u96f6\u7121\u7d71\u8a08\u4e0a\u7684\u5dee\u7570\u3002<\/li>\n<li><strong>\u6b98\u5dee\uff1a<\/strong> \u6b98\u5dee\u5716\u7121\u7cfb\u7d71\u6027\u66f2\u7387\uff1b\u5982\u679c\u4f60\u770b\u5230\u66f2\u7387\uff0c\u5075\u6e2c\u5668\u7dda\u6027\u5728\u7bc4\u570d\u7684\u4e00\u7aef\u5931\u6557\u4e86\u3002<\/li>\n<\/ul>\n\n<h3>\u7dda\u6027\u5931\u6557\u9577\u4ec0\u9ebc\u6a23<\/h3>\n<p>\u6700\u5e38\u898b\u7684\u5931\u6557\u6a21\u5f0f\uff1a\u5728\u9ad8\u5438\u5149\u5ea6\uff081.5 AU \u4ee5\u4e0a\uff09\uff0c\u66f2\u7dda\u8b8a\u5e73\u2014\u2014\u91cf\u5230\u7684 A \u6bd4\u6fc3\u5ea6\u9810\u6e2c\u7684\u4f4e\u3002\u9019\u662f\u5075\u6e2c\u5668\u98fd\u548c\u3001\u5100\u5668\u7684\u6975\u9650\u3002\u88dc\u6551\u662f\u5728\u7dda\u6027\u7bc4\u570d\u5167\u64cd\u4f5c\uff08\u7528\u66f4\u77ed\u7684\u6bd4\u8272\u76bf\u3001\u7a00\u91cb\u6a23\u54c1\uff0c\u6216\u63a5\u53d7\u9ad8 AU \u8b80\u6578\u50c5\u4f9b\u7be9\u6aa2\uff09\u3002<\/p><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 340\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg4-t\"><title id=\"svg4-t\">\u96dc\u6563\u5149\u622a\u6b62\u6ffe\u5149\u7247\u5149\u8b5c\uff0c\u986f\u793a KCl\u3001NaI \u8207\u6c34\u6e2c\u8a66\u6eb6\u6db2\u5728\u7279\u5fb5\u7d2b\u5916\u6ce2\u9577\u5438\u5149\u5ea6\u5347\u5230\u9ad8\u503c\uff0c\u7528\u65bc USP 851 \u96dc\u6563\u5149\u9a57\u8b49<\/title><rect width=\"720\" height=\"340\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u96dc\u6563\u5149\u9a57\u8b49 \u2014 \u7d2b\u5916\u622a\u6b62\u6e2c\u8a66\u6eb6\u6db2<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">\u5f37\u622a\u6b62\u7269\u8cea\u53ea\u6709\u5728\u96dc\u6563\u5149\u4f4e\u5230\u53ef\u63a5\u53d7\u6642\u624d\u986f\u793a\u91cf\u5230 A \u9ad8\u65bc 1.5 AU<\/text><rect x=\"80\" y=\"60\" width=\"600\" height=\"220\" fill=\"#fff\" stroke=\"#cdd2dd\" stroke-width=\"1\"\/><g stroke=\"#e8eaf0\" stroke-width=\"0.6\"><line x1=\"80\" y1=\"100\" x2=\"680\" y2=\"100\"\/><line x1=\"80\" y1=\"160\" x2=\"680\" y2=\"160\"\/><line x1=\"80\" y1=\"220\" x2=\"680\" y2=\"220\"\/><line x1=\"200\" y1=\"60\" x2=\"200\" y2=\"280\"\/><line x1=\"320\" y1=\"60\" x2=\"320\" y2=\"280\"\/><line x1=\"440\" y1=\"60\" x2=\"440\" y2=\"280\"\/><line x1=\"560\" y1=\"60\" x2=\"560\" y2=\"280\"\/><\/g><line x1=\"80\" y1=\"280\" x2=\"680\" y2=\"280\" stroke=\"#333\" stroke-width=\"1.2\"\/><line x1=\"80\" y1=\"60\" x2=\"80\" y2=\"280\" stroke=\"#333\" stroke-width=\"1.2\"\/><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"80\" y=\"296\" text-anchor=\"middle\">190<\/text><text x=\"200\" y=\"296\" text-anchor=\"middle\">220<\/text><text x=\"320\" y=\"296\" text-anchor=\"middle\">250<\/text><text x=\"440\" y=\"296\" text-anchor=\"middle\">280<\/text><text x=\"560\" y=\"296\" text-anchor=\"middle\">320<\/text><text x=\"680\" y=\"296\" text-anchor=\"middle\">360<\/text><\/g><text x=\"380\" y=\"314\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\">\u6ce2\u9577\uff08nm\uff09<\/text><text x=\"36\" y=\"170\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-weight=\"600\" fill=\"#333\" text-anchor=\"middle\" transform=\"rotate(-90 36 170)\">\u5438\u5149\u5ea6\uff08AU\u300110 mm \u6bd4\u8272\u76bf\uff09<\/text><g font-family=\"Arial,sans-serif\" font-size=\"10\" fill=\"#555\"><text x=\"74\" y=\"64\" text-anchor=\"end\">2.5<\/text><text x=\"74\" y=\"124\" text-anchor=\"end\">1.5<\/text><text x=\"74\" y=\"184\" text-anchor=\"end\">0.8<\/text><text x=\"74\" y=\"244\" text-anchor=\"end\">0.2<\/text><\/g><line x1=\"80\" y1=\"120\" x2=\"680\" y2=\"120\" stroke=\"#16a34a\" stroke-width=\"1.5\" stroke-dasharray=\"6 3\" opacity=\"0.6\"\/><text x=\"690\" y=\"124\" text-anchor=\"end\" font-family=\"Arial,sans-serif\" font-size=\"10\" font-weight=\"700\" fill=\"#16a34a\">A > 1.5 = \u901a\u904e<\/text><path d=\"M 80 80 L 130 100 L 160 200 L 200 270 L 280 277 L 680 278\" stroke=\"#dc2626\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"100\" y=\"92\" font-family=\"Arial,sans-serif\" font-size=\"10\" font-weight=\"700\" fill=\"#dc2626\">1.2% KCl<\/text><text x=\"100\" y=\"105\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#dc2626\">\u622a\u6b62 @ 198 nm<\/text><path d=\"M 80 70 L 110 75 L 150 90 L 200 100 L 240 240 L 280 275 L 380 277 L 680 278\" stroke=\"#1a2a6c\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"220\" y=\"85\" font-family=\"Arial,sans-serif\" font-size=\"10\" font-weight=\"700\" fill=\"#1a2a6c\">1% NaI<\/text><text x=\"220\" y=\"98\" font-family=\"Arial,sans-serif\" font-size=\"9\" fill=\"#1a2a6c\">\u622a\u6b62 @ 220 nm<\/text><path d=\"M 80 95 L 110 110 L 140 200 L 180 270 L 250 277 L 680 278\" stroke=\"#7c3aed\" stroke-width=\"2.4\" fill=\"none\"\/><text x=\"100\" y=\"178\" font-family=\"Arial,sans-serif\" font-size=\"10\" font-weight=\"700\" fill=\"#7c3aed\">\u6c34\uff08200 nm \u622a\u6b62\uff09<\/text><\/svg><figcaption>\u5716 4 \u2014 \u96dc\u6563\u5149\u9a57\u8b49\u3002\u5f37\u622a\u6b62\u6ffe\u5149\u7247\uff081.2% KCl\u30011% NaI\u3001\u7d14\u6c34\uff09\u57fa\u672c\u4e0a\u5438\u6536\u5176\u622a\u6b62\u6ce2\u9577\u4ee5\u4e0a\u7684\u6240\u6709\u8f3b\u5c04\u3002\u5982\u679c\u5206\u5149\u5149\u5ea6\u8a08\u7684\u96dc\u6563\u5149\u53ef\u63a5\u53d7\uff0c\u622a\u6b62\u4ee5\u4e0a\u91cf\u5230\u7684\u5438\u5149\u5ea6\u8d85\u904e 1.5 AU\uff1b\u5982\u679c\u96dc\u6563\u5149\u592a\u9ad8\uff0c\u672a\u88ab\u64cb\u4f4f\u7684\u96dc\u6563\u5149\u5b50\u5230\u9054\u5075\u6e2c\u5668\u3001\u91cf\u5230\u7684\u5438\u5149\u5ea6\u5047\u6027\u504f\u4f4e\u3002<\/figcaption><\/figure><h2 id=\"stray\">6. \u96dc\u6563\u5149 \u2014 \u622a\u6b62\u6ffe\u5149\u7247\u6e2c\u8a66<\/h2>\n<p>\u96dc\u6563\u5149\u662f\u5728\u6240\u9078\u6ce2\u9577\u983b\u5bec\u4e4b\u5916\u5230\u9054\u5075\u6e2c\u5668\u7684\u96fb\u78c1\u8f3b\u5c04\u3002\u539f\u56e0\u5305\u62ec\u55ae\u8272\u5668\u6563\u5c04\u3001\u5149\u675f\u8655\u7406\u5149\u5b78\u7684\u4e0d\u5b8c\u7f8e\uff0c\u8207\u74b0\u5883\u5149\u6f0f\u9032\u6a23\u54c1\u5ba4\u3002\u5373\u4f7f\u5f88\u5c0f\u7684\u96dc\u6563\u5149\u6bd4\u4f8b\uff080.01%\uff09\u4e5f\u6703\u9020\u6210\u9ad8\u5438\u5149\u5ea6\u8b80\u6578\u7684\u7cfb\u7d71\u6027\u4f4e\u4f30\u3002<\/p>\n\n<h3>\u622a\u6b62\u6ffe\u5149\u7247\u539f\u7406<\/h3>\n<p>\u628a\u4e00\u500b\u57fa\u672c\u4e0a\u5438\u6536\u5176\u622a\u6b62\u6ce2\u9577\u4ee5\u4e0a\u6240\u6709\u8f3b\u5c04\u7684\u7269\u8cea\u653e\u9032\u6a23\u54c1\u5149\u675f\u3002\u7406\u60f3\u96dc\u6563\u5149 = 0 \u6642\uff0c\u91cf\u5230\u7684\u5438\u5149\u5ea6\u5c31\u662f\u8a72\u7269\u8cea\u5728\u90a3\u6ce2\u9577\u7684\u771f\u5be6\u5438\u5149\u5ea6\u2014\u2014\u5f37\u622a\u6b62\u901a\u5e38 > 4 AU\u3002\u96dc\u6563\u5149\u986f\u8457\u6642\uff0c\u91cf\u5230\u7684\u5438\u5149\u5ea6\u5728\u5c0d\u61c9\u96dc\u6563\u5149\u767e\u5206\u6bd4\u7684\u503c\u8b8a\u5e73\uff08\u4f8b\u5982 0.1% \u96dc\u6563\u5149\u628a\u91cf\u5230\u7684 A \u5c01\u9802\u5728 3.0 AU\uff1b1% \u5728 2.0 AU\uff1b2% \u5728 ~1.7 AU\uff09\u3002<\/p>\n\n<h3>USP <851> \u53c3\u8003\u6eb6\u6db2<\/h3>\n<table class=\"csg-protocol\">\n<thead><tr><th>\u53c3\u8003<\/th><th>\u6fc3\u5ea6<\/th><th>\u622a\u6b62 \u03bb<\/th><th>\u901a\u904e\u6a19\u6e96<\/th><\/tr><\/thead>\n<tbody>\n<tr><td>\u6c2f\u5316\u9240\uff08KCl\uff09<\/td><td>\u6c34\u4e2d 1.2% w\/v<\/td><td>198 nm<\/td><td>198 nm \u8655 A > 2.0<\/td><\/tr>\n<tr><td>\u7898\u5316\u9209\uff08NaI\uff09<\/td><td>\u6c34\u4e2d 1.0% w\/v<\/td><td>220 nm<\/td><td>220 nm \u8655 A > 1.5<\/td><\/tr>\n<tr><td>\u4e9e\u785d\u9178\u9209\uff08NaNO\u2082\uff09<\/td><td>\u6c34\u4e2d 5.0% w\/v<\/td><td>340 nm<\/td><td>340 nm \u8655 A > 1.5\uff08\u53ef\u898b\u908a\u7de3\u96dc\u6563\u5149\uff09<\/td><\/tr>\n<tr><td>\u7d14\u53bb\u96e2\u5b50\u6c34<\/td><td>\uff08\u7a7a\u767d\uff09<\/td><td>200 nm<\/td><td>\u5408\u7406\u7684\u57fa\u7dda\uff1b\u672c\u8eab\u4e0d\u7b97\u622a\u6b62<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<div class=\"csg-callout\"><strong>\u300c\u96dc\u6563\u5149\u5931\u6557\u300d\u5728\u5be6\u52d9\u4e0a\u662f\u4ec0\u9ebc\u610f\u601d\u3002<\/strong> \u5982\u679c\u96dc\u6563\u5149\u592a\u9ad8\uff0c\u4f60\u5728\u53d7\u5f71\u97ff\u7684\u6ce2\u9577\u5340\u7121\u6cd5\u4fe1\u4efb ~2 AU \u4ee5\u4e0a\u7684\u5438\u5149\u5ea6\u8b80\u6578\u3002\u5c0d\u4f60\u7784\u6e96 0.5\u20131.0 AU \u7684\u88fd\u85e5\u6e2c\u5b9a\uff0c\u9019\u5f88\u5c11\u662f\u554f\u984c\u3002\u5c0d\u9ad8\u5438\u5149\u5ea6\u7684\u5fae\u91cf\u5de5\u4f5c\u6216\u96dc\u8cea\u5206\u6790\uff0c\u96dc\u6563\u5149\u9a57\u8b49\u66f4\u91cd\u8981\u3002<\/div><h2 id=\"resolution\">7. \u89e3\u6790\u5ea6 \u2014 \u5df1\u70f7\u4e2d\u7532\u82ef\u6e2c\u8a66<\/h2>\n<p>\u5149\u8b5c\u983b\u5bec\uff08SBW\uff09\u6c7a\u5b9a\u5169\u500b\u76f8\u9130\u7684\u7a84\u5438\u6536\u5cf0\u662f\u5206\u8fa8\u6210\u7368\u7acb\u7279\u5fb5\u3001\u9084\u662f\u4f75\u6210\u4e00\u500b\u5bec\u80a9\u3002\u7a84 SBW \u7d66\u66f4\u597d\u7684\u89e3\u6790\u5ea6\u3001\u4f46\u8f03\u4f4e\u7684\u901a\u91cf\uff08\u9032\u5075\u6e2c\u5668\u7684\u5149\u8f03\u5c11\uff09\uff1b\u9019\u53d6\u6368\u7531\u5149\u8b5c\u5100\u88fd\u9020\u5546\u7684\u8a2d\u8a08\u6c7a\u5b9a\u3002<\/p>\n<h3>\u7532\u82ef\/\u5df1\u70f7\u89e3\u6790\u5ea6\u6e2c\u8a66<\/h3>\n<p>\u5df1\u70f7\u4e2d 0.020% v\/v \u7532\u82ef <em>n<\/em>\u5728 269 \u8207 266 nm \u986f\u793a\u5169\u500b\u7279\u5fb5\u5438\u6536\u5cf0\u3001\u4e4b\u9593\u6709\u500b\u5c0f\u8c37\u3002A269 \/ A266 \u7684\u6bd4\u503c\u662f\u89e3\u6790\u5ea6\u6307\u6a19\uff1a<\/p>\n<ul>\n<li><strong>USP <851> \u901a\u904e\u6a19\u6e96\uff1a<\/strong> A269 \/ A266 \u2265 1.5<\/li>\n<li><strong>EP 2.2.25 \u901a\u904e\u6a19\u6e96\uff1a<\/strong> A269 \/ A266 \u2265 1.5<\/li>\n<li><strong>\u8f03\u597d\u7684\u5100\u5668\uff1a<\/strong> \u6bd4\u503c \u2265 2.0\uff08\u66f4\u7a84 SBW\u3001\u66f4\u597d\u89e3\u6790\u5ea6\uff09<\/li>\n<\/ul>\n<p>\u5982\u679c\u6bd4\u503c\u8dcc\u5230 1.5 \u4ee5\u4e0b\uff0c\u5149\u8b5c\u5100\u7684 SBW \u5c0d\u53d7\u898f\u7bc4\u65b9\u6cd5\u592a\u5bec\uff1b\u5100\u5668\u7121\u6cd5\u53ef\u9760\u5730\u5340\u5206\u7dca\u9130\u7684\u5cf0\u3002\u4fee\u6cd5\u662f\u63db\u5230\u66f4\u7a84 SBW \u7684\u5100\u5668\uff0c\u6216\u5982\u679c\u5100\u5668\u652f\u63f4\u5c31\u628a SBW \u8a2d\u5230\u66f4\u7a84\u7684\u503c\uff08\u67d0\u4e9b\u6383\u63cf\u5f0f\u5149\u8b5c\u5100\u63d0\u4f9b 0.5\u30011.0\u30012.0\u30014.0 nm \u8a2d\u5b9a\uff09\u3002<\/p><h2 id=\"pathlength\">8. \u6bd4\u8272\u76bf\u5149\u7a0b\u9a57\u8b49<\/h2>\n<p>\u85e5\u5178\u7684\u5206\u5149\u5149\u5ea6\u8a08\u898f\u7a0b\u5c0d\u6bd4\u8272\u76bf\u5149\u7a0b\u9a57\u8b49\u96bb\u5b57\u672a\u63d0\u2014\u2014\u5b83\u5011\u5047\u8a2d\u6bd4\u8272\u76bf\u5c31\u662f\u5176\u6a19\u7c64\u8aaa\u7684\u3002\u5be6\u52d9\u4e0a\uff0c\u4e09\u4ef6\u4e8b\u53ef\u80fd\u8b8a\u52d5\uff1a\u6bd4\u8272\u76bf\u5728\u88fd\u9020\u6642\u88ab\u91cf\u932f\uff08\u4fe1\u8b7d\u597d\u7684\u4f9b\u61c9\u5546\u5c11\u898b\uff09\u3001\u7a97\u7247\u5728\u591a\u5e74\u4f7f\u7528\u4e2d\u767c\u5c55\u51fa\u5fae\u66f2\u7387\uff0c\u6216\u6bd4\u8272\u76bf\u8207\u9577\u5f97\u50cf\u4f46\u5149\u7a0b\u4e0d\u540c\u7684\u6bd4\u8272\u76bf\u641e\u6df7\u3002\u5149\u7a0b\u9a57\u8b49\u6293\u4f4f\u9019\u4e09\u8005\u3002<\/p>\n\n<h3>\u65b9\u6cd5 1\uff1a\u4f9b\u61c9\u5546\u5206\u6790\u8b49\u66f8\uff08CoA\uff09<\/h3>\n<p>\u6700\u7c21\u55ae\u7684\u9a57\u8b49\uff1a\u6bcf\u500b\u4fe1\u8b7d\u597d\u7684\u6bd4\u8272\u76bf\u88fd\u9020\u5546\u90fd\u9644\u4e00\u4efd CoA\uff0c\u8f09\u660e\u91cf\u5230\u7684\u5149\u7a0b\u5230 \u00b10.005 cm\uff08\u5178\u578b\uff09\u6216 \u00b10.002 cm\uff08\u9ad8\u968e\uff09\u5bb9\u5dee\u3002\u628a CoA \u9023\u540c\u6bd4\u8272\u76bf\u5e8f\u865f\u5b58\u6a94\uff1b\u5728\u9032\u6599\u6aa2\u9a57\u6216\u6bcf\u5e74\u5be9\u6838\u3002\u4f8b\u884c\u4f7f\u7528\u53ef\u63a5\u53d7\uff0c\u4f46\u6293\u4e0d\u5230\u4f7f\u7528\u4e2d\u7684\u6f02\u79fb\u3002\u898b\u6211\u5011\u7684 <a href=\"https:\/\/machinedquartz.com\/bulk-quartz-cuvettes\/\">\u6279\u91cf\u8207 OEM \u9801<\/a> \u4e86\u89e3\u6587\u4ef6\u9078\u9805\u3002<\/p>\n\n<h3>\u65b9\u6cd5 2\uff1a\u7528\u53c3\u8003\u6a19\u6e96\u505a\u6717\u4f2f-\u6bd4\u723e\u9a57\u8b49<\/h3>\n<p>\u628a\u6bd4\u8272\u76bf\u586b\u5165\u5df2\u77e5\u6fc3\u5ea6\u7684 K\u2082Cr\u2082O\u2087 \u53c3\u8003\u6eb6\u6db2\u3002\u5728 257 nm \u91cf\u5438\u5149\u5ea6\u3002\u8a08\u7b97\u5149\u7a0b\uff1a<\/p>\n<p style=\"text-align:center;font-family:monospace;background:#f7f8fc;padding:14px;border-radius:6px;\"><strong>l (cm) = A\u2084\u2086\u2087 \/ (A\u00b9% \u00b7 c)<\/strong><\/p>\n<p>\u5176\u4e2d c \u662f % w\/v \u7684\u6fc3\u5ea6\u3001257 nm \u7684 A\u00b9% = 144.0\u3002\u628a\u7b97\u51fa\u7684 l \u8207\u6a19\u7c64\u5149\u7a0b\u6bd4\u8f03\u3002\u5dee\u7570 < \u00b12% \u8868\u793a\u5149\u7a0b\u5728\u5bb9\u5dee\u5167\u3002\u9019\u65b9\u6cd5\u8981\u6c42\u5149\u8b5c\u5100\u672c\u8eab\u5df2\u9a57\u8b49\u904e\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u3002<\/p>\n\n<h3>\u65b9\u6cd5 3\uff1a\u5dee\u5206\u6bd4\u8f03<\/h3>\n<p>\u5c0d\u96d9\u5149\u675f\u5149\u8b5c\u5100\uff0c\u628a\u6e2c\u8a66\u6bd4\u8272\u76bf\uff08\u586b K\u2082Cr\u2082O\u2087\uff09\u653e\u9032\u6a23\u54c1\u5149\u675f\u3001\u53c3\u8003\u6bd4\u8272\u76bf\uff08\u586b\u540c\u6a23\u6eb6\u6db2\u3001\u5149\u7a0b\u5f9e CoA \u5df2\u77e5\uff09\u653e\u9032\u53c3\u8003\u5149\u675f\u3002\u91cf\u5230\u7684 A \u61c9\u63a5\u8fd1\u96f6\u3002\u4efb\u4f55\u7cfb\u7d71\u6027\u504f\u79fb\u5c0d\u61c9\u4e00\u500b\u5149\u7a0b\u5dee\uff1a\u0394A = A\u00b9% \u00b7 c \u00b7 (l_\u6a23\u54c1 \u2212 l_\u53c3\u8003)\u3002\u9019\u662f\u6700\u9748\u654f\u7684\u81ea\u6709\u65b9\u6cd5\uff0c\u4f8b\u884c\u5c31\u80fd\u6293\u5230\u4e0d\u5230\u4e00\u500b\u767e\u5206\u9ede\u7684\u5149\u7a0b\u6f02\u79fb\u3002<\/p>\n\n<h3>\u65b9\u6cd5 4\uff1a\u5149\u5b78\u5e72\u6d89\u6cd5\uff08\u7279\u6b8a\uff09<\/h3>\n<p>\u7a7a\u6bd4\u8272\u76bf\uff1a\u653e\u5728\u5169\u7247\u53cd\u5c04\u5149\u5b78\u5e73\u677f\u4e4b\u9593\u89c0\u5bdf\u725b\u9813\u74b0\uff0c\u6216\u7528\u9081\u514b\u751f\u5e72\u6d89\u5100\u91cf\u6e2c\u3002\u7d66\u51fa\u7d55\u5c0d\u5149\u7a0b\u5230 \u00b10.5 \u00b5m\u3002\u50c5\u7279\u6b8a\u5be6\u9a57\u5ba4\uff1b\u5c0d\u88fd\u85e5\u54c1\u7ba1\u975e\u4f8b\u884c\u3002\u53ef\u5f9e\u5149\u5b78\u6e2c\u8a66\u8a2d\u5099\u4f9b\u61c9\u5546\u53d6\u5f97\u6b64\u670d\u52d9\u3002<\/p><h2 id=\"matching\">9. \u6bd4\u8272\u76bf\u914d\u5c0d\u5339\u914d<\/h2>\n<p>\u96d9\u5149\u675f\u5206\u5149\u5149\u5ea6\u8a08\u540c\u6642\u8dd1\u5169\u652f\u6bd4\u8272\u76bf\uff1a\u6a23\u54c1\u5728\u4e00\u500b\u5149\u675f\u3001\u7a7a\u767d\u6216\u53c3\u8003\u5728\u53e6\u4e00\u500b\u3002\u5100\u5668\u8a08\u7b97 A = -log(I_\u6a23\u54c1 \/ I_\u53c3\u8003)\u3002\u8981\u8b93\u9019\u6210\u7acb\uff0c\u6a23\u54c1\u8207\u53c3\u8003\u6bd4\u8272\u76bf\u5fc5\u9808\u5728\u95dc\u6ce8\u7684\u6ce2\u9577\u7bc4\u570d\u5167\u7b49\u6548\u5730\u900f\u5c04\u3002\u4e0d\u5339\u914d\u7684\u914d\u5c0d\u9020\u6210\u770b\u8d77\u4f86\u50cf\u6a23\u54c1\u5438\u5149\u5ea6\u3001\u4f46\u5176\u5be6\u4e0d\u662f\u7684\u7cfb\u7d71\u6027\u57fa\u7dda\u8aa4\u5dee\u3002<\/p>\n\n<h3>\u300c\u5339\u914d\u300d\u662f\u4ec0\u9ebc\u610f\u601d<\/h3>\n<ul>\n<li>\u5149\u7a0b\u76f8\u540c\u3001\u5728 \u00b10.005 cm \u5167\uff08\u901a\u5e38\u7531\u88fd\u9020\u5546\u7dad\u6301\uff09<\/li>\n<li>\u7a97\u7247\u539a\u5ea6\u76f8\u540c\u3001\u5728 \u00b10.05 mm \u5167<\/li>\n<li>\u7a97\u7247\u5e73\u6574\u5ea6\u76f8\u540c\uff08\u7121\u5167\u90e8\u6954\u5f62\uff09<\/li>\n<li>\u8868\u9762\u5149\u6f54\u5ea6\u76f8\u540c\uff08\u6e05\u6f54\u53f2\u3001\u7121\u522e\u75d5\uff09<\/li>\n<li>\u900f\u904e\u7387\u5339\u914d\uff1a\u8de8\u5de5\u4f5c\u5149\u8b5c \u0394A < 0.005\uff08\u7576\u5169\u652f\u90fd\u586b\u540c\u6a23\u7684\u7a7a\u767d\uff09<\/li>\n<\/ul>\n\n<h3>\u9a57\u8b49\u4e00\u5c0d<\/h3>\n<ol>\n<li>\u5169\u652f\u6bd4\u8272\u76bf\u90fd\u586b\u540c\u6a23\u7684\u7a7a\u767d\u6eb6\u5291\uff08\u6c34\u76f8\u65b9\u6cd5\u901a\u5e38\u53bb\u96e2\u5b50\u6c34\uff0c\u6216\u5339\u914d\u7684\u6eb6\u5291\uff09\u3002<\/li>\n<li>\u4e00\u652f\u653e\u6a23\u54c1\u5149\u675f\u3001\u53e6\u4e00\u652f\u653e\u53c3\u8003\u5149\u675f\u3002\u8dd1\u57fa\u7dda\u6383\u63cf\u3002<\/li>\n<li>\u8de8 200\u2013800 nm \u91cf\u5230\u7684 A \u61c9\u662f 0.000 \u00b1 0.005\u3002\u4efb\u4f55\u504f\u5dee\u5c31\u662f\u914d\u5c0d\u4e0d\u5339\u914d\u3002<\/li>\n<li>\u628a\u6bd4\u8272\u76bf\u5c0d\u8abf\uff08\u6a23\u54c1 \u2194 \u53c3\u8003\uff09\u91cd\u8907\u3002\u504f\u5dee\u7684\u6b63\u8ca0\u865f\u61c9\u53cd\u8f49\uff1b\u5927\u5c0f\u61c9\u543b\u5408\u3002<\/li>\n<\/ol>\n\n<h3>\u91cd\u65b0\u5339\u914d\u6f02\u958b\u7684\u914d\u5c0d<\/h3>\n<p>\u7528\u4e86\u597d\u5e7e\u5e74\u7684\u914d\u5c0d\u5e38\u56e0\u5176\u4e2d\u4e00\u652f\u7d2f\u7a4d\u8f03\u591a\u78e8\u640d\u800c\u6f02\u958b\u3002\u5982\u679c\u4e00\u500b\u6709\u6587\u4ef6\u8a18\u8f09\u7684\u914d\u5c0d\u6c92\u901a\u904e \u0394A < 0.005 \u6aa2\u67e5\uff0c\u9078\u9805\u662f\uff1a(a) \u628a\u4e00\u652f\u5f9e\u914d\u5c0d\u4e2d\u63db\u6389\u3001\u7528\u4e00\u652f\u65b0\u7684\u8207\u5269\u4e0b\u7684\u91cd\u65b0\u5339\u914d\uff1b(b) \u5411\u88fd\u9020\u5546\u91cd\u65b0\u8cfc\u8cb7\u4e00\u5c0d\uff1b(c) \u628a\u6bd4\u8272\u76bf\u7576\u55ae\u652f\u7528\u65bc\u4e0d\u9069\u7528\u914d\u5c0d\u5339\u914d\u7684\u55ae\u5149\u675f\u5de5\u4f5c\u3002<\/p>\n\n<div class=\"csg-callout tip\"><strong>\u8cb7\u9810\u5148\u5339\u914d\u7684\u914d\u5c0d\u3002<\/strong> \u591a\u6578\u4fe1\u8b7d\u597d\u7684\u6bd4\u8272\u76bf\u88fd\u9020\u5546\uff08\u5305\u62ec MachinedQuartz \u4f9d\u9700\u6c42\uff09\u8ca9\u552e\u8de8\u5b8c\u6574 200\u2013800 nm \u5149\u8b5c\u6709\u6587\u4ef6\u8a18\u8f09 \u0394A \u7684\u9810\u5339\u914d\u914d\u5c0d\u3002\u6bcf\u652f\u52a0\u4e00\u9ede\u50f9\uff0c\u4f46\u7701\u4e0b\u81ea\u5df1\u5339\u914d\u7684\u5de5\u4f5c\u3001\u4e26\u70ba\u78ba\u6548\u6a94\u6848\u63d0\u4f9b\u76f4\u63a5\u7684\u6587\u4ef6\u3002<\/div><figure class=\"csg-svg-figure\"><svg viewBox=\"0 0 720 320\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"svg5-t\"><title id=\"svg5-t\">\u9a57\u8b49\u983b\u7387\u6642\u9593\u8ef8\uff0c\u986f\u793a\u53d7\u898f\u7bc4 GMP \u74b0\u5883\u4e2d\u6bd4\u8272\u76bf\u8207\u5206\u5149\u5149\u5ea6\u8a08\u7684\u6bcf\u65e5\u3001\u6bcf\u9031\u3001\u6bcf\u6708\u3001\u6bcf\u5b63\u8207\u6bcf\u5e74\u6aa2\u67e5<\/title><rect width=\"720\" height=\"320\" fill=\"#ffffff\"\/><text x=\"360\" y=\"22\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"15\" font-weight=\"700\" fill=\"#1a2a6c\">\u9a57\u8b49\u983b\u7387 \u2014 \u4f55\u6642\u505a\u4ec0\u9ebc<\/text><text x=\"360\" y=\"40\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" fill=\"#666\">GMP \u53d7\u898f\u7bc4 UV-Vis \u5206\u5149\u5149\u5ea6\u8a08 + \u6bd4\u8272\u76bf\u7d44\u7684\u5178\u578b\u6642\u7a0b<\/text><g font-family=\"Arial,sans-serif\"><g><rect x=\"20\" y=\"60\" width=\"130\" height=\"220\" fill=\"#fef3c7\" stroke=\"#ca8a04\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"85\" y=\"84\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#854d0e\">\u6bcf\u65e5<\/text><text x=\"85\" y=\"100\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">\u4f7f\u7528\u65e5<\/text><text x=\"85\" y=\"124\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u7cfb\u7d71\u9069\u7528\u6027<\/text><text x=\"85\" y=\"142\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u54c1\u7ba1\u6a19\u6e96<\/text><text x=\"85\" y=\"156\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u5728\u5df2\u77e5 A<\/text><text x=\"85\" y=\"180\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u6bd4\u8272\u76bf\u6aa2\u67e5<\/text><text x=\"85\" y=\"198\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u71c8\u4e0b\u76ee\u8996<\/text><text x=\"85\" y=\"212\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u7121\u522e\u75d5 \/ \u8584\u819c<\/text><text x=\"85\" y=\"240\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u71c8\u9810\u71b1<\/text><text x=\"85\" y=\"258\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u6700\u5c11 30 \u5206\u9418<\/text><\/g><g><rect x=\"160\" y=\"60\" width=\"130\" height=\"220\" fill=\"#dbeafe\" stroke=\"#1a2a6c\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"225\" y=\"84\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#1a2a6c\">\u6bcf\u9031<\/text><text x=\"225\" y=\"100\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">~5 \u5929<\/text><text x=\"225\" y=\"124\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u6ce2\u9577\u5feb\u67e5<\/text><text x=\"225\" y=\"142\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u9225\u73bb\u7483<\/text><text x=\"225\" y=\"156\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u6383 240-650<\/text><text x=\"225\" y=\"180\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u914d\u5c0d\u5339\u914d<\/text><text x=\"225\" y=\"198\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u6c34-\u6c34\u7a7a\u767d<\/text><text x=\"225\" y=\"212\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u0394A < 0.005<\/text><\/g><g><rect x=\"300\" y=\"60\" width=\"130\" height=\"220\" fill=\"#dcfce7\" stroke=\"#16a34a\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"365\" y=\"84\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#15803d\">\u6bcf\u6708<\/text><text x=\"365\" y=\"100\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">~22 \u5929<\/text><text x=\"365\" y=\"124\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u6ce2\u9577\u5b8c\u6574<\/text><text x=\"365\" y=\"142\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u9225\u904e\u6c2f\u9178<\/text><text x=\"365\" y=\"156\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u6eb6\u6db2 USP<\/text><text x=\"365\" y=\"180\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6<\/text><text x=\"365\" y=\"198\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">K\u2082Cr\u2082O\u2087 257 nm<\/text><text x=\"365\" y=\"212\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u00b12% \u76f8\u5c0d<\/text><text x=\"365\" y=\"240\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u96dc\u6563\u5149<\/text><text x=\"365\" y=\"258\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">KCl\/NaI \u622a\u6b62<\/text><\/g><g><rect x=\"440\" y=\"60\" width=\"130\" height=\"220\" fill=\"#e0e7ff\" stroke=\"#4338ca\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"505\" y=\"84\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#3730a3\">\u6bcf\u5b63<\/text><text x=\"505\" y=\"100\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">~64 \u5929<\/text><text x=\"505\" y=\"124\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u7dda\u6027<\/text><text x=\"505\" y=\"142\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">K\u2082Cr\u2082O\u2087 \u7cfb\u5217<\/text><text x=\"505\" y=\"156\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">r\u00b2 \u2265 0.999<\/text><text x=\"505\" y=\"180\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u89e3\u6790\u5ea6<\/text><text x=\"505\" y=\"198\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u7532\u82ef\/\u5df1\u70f7<\/text><text x=\"505\" y=\"212\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">A269\/A266 \u22651.5<\/text><text x=\"505\" y=\"240\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u6bd4\u8272\u76bf\u5149\u7a0b l<\/text><text x=\"505\" y=\"258\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u5c0d CoA \/ \u6a19\u6e96<\/text><\/g><g><rect x=\"580\" y=\"60\" width=\"130\" height=\"220\" fill=\"#fce7f3\" stroke=\"#be185d\" stroke-width=\"1.5\" rx=\"8\"\/><text x=\"645\" y=\"84\" text-anchor=\"middle\" font-size=\"13\" font-weight=\"700\" fill=\"#9d174d\">\u6bcf\u5e74<\/text><text x=\"645\" y=\"100\" text-anchor=\"middle\" font-size=\"9\" fill=\"#666\">12 \u500b\u6708<\/text><text x=\"645\" y=\"124\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u5b8c\u6574 IQ\/OQ<\/text><text x=\"645\" y=\"142\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u4f9b\u61c9\u5546\u6216<\/text><text x=\"645\" y=\"156\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u7b2c\u4e09\u65b9\u7dad\u4fee<\/text><text x=\"645\" y=\"180\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">\u63db\u71c8<\/text><text x=\"645\" y=\"198\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">D\u2082 \u71c8 2000h<\/text><text x=\"645\" y=\"212\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u93a2\u71c8 5000h<\/text><text x=\"645\" y=\"240\" text-anchor=\"middle\" font-size=\"11\" font-weight=\"700\" fill=\"#1a1a2e\">SOP \u5be9\u6838<\/text><text x=\"645\" y=\"258\" text-anchor=\"middle\" font-size=\"10\" fill=\"#444\">\u5c0d\u7576\u524d\u65b9\u6cd5<\/text><\/g><\/g><text x=\"360\" y=\"306\" text-anchor=\"middle\" font-family=\"Arial,sans-serif\" font-size=\"11\" font-style=\"italic\" fill=\"#666\">\u983b\u7387\u4f9d\u5be6\u9a57\u5ba4 GxP \u7b49\u7d1a\u800c\u7570\u2014\u2014\u4f9d\u4f60\u7684\u54c1\u8cea\u7cfb\u7d71\u8981\u6c42\u8abf\u6574\u3002<\/text><\/svg><figcaption>\u5716 5 \u2014 \u9a57\u8b49\u983b\u7387\u6642\u9593\u8ef8\u3002\u6bcf\u65e5\u6aa2\u67e5\u5728\u91cf\u6e2c\u524d\u78ba\u8a8d\u7cfb\u7d71\u9069\u7528\u6027\u3002\u6bcf\u9031\u6aa2\u67e5\u6293\u77ed\u671f\u6f02\u79fb\u3002\u6bcf\u6708\u6aa2\u67e5\u662f\u591a\u6578\u88fd\u85e5\u54c1\u7ba1\u7684\u6cd5\u898f\u6700\u4f4e\u8981\u6c42\u3002\u6bcf\u5b63\u6aa2\u67e5\u6db5\u84cb\u6162\u6f02\u79fb\u9805\u76ee\uff08\u7dda\u6027\u3001\u89e3\u6790\u5ea6\u3001\u5149\u7a0b\uff09\u3002\u6bcf\u5e74\u6d89\u53ca\u4f9b\u61c9\u5546\u7dad\u4fee\u3001\u63db\u71c8\uff0c\u8207\u5c0d\u7576\u524d\u85e5\u5178\u65b9\u6cd5\u7684 SOP \u5be9\u6838\u3002<\/figcaption><\/figure><h2 id=\"frequency\">10. \u9a57\u8b49\u983b\u7387 \u2014 \u4f55\u6642\u505a\u4ec0\u9ebc<\/h2>\n<p>\u85e5\u5178\u65b9\u6cd5\u63cf\u8ff0\u9a57\u8b49\u4ec0\u9ebc\u3001\u4f46\u4e0d\u8b1b\u591a\u4e45\u4e00\u6b21\u3002\u983b\u7387\u7531\u5be6\u9a57\u5ba4\u7684\u54c1\u8cea\u7cfb\u7d71\u3001\u91cf\u6e2c\u7684\u95dc\u9375\u6027\uff0c\u8207\u5100\u5668\u7684\u5931\u6548\u7387\u6c7a\u5b9a\u3002\u4e0b\u9762\u662f GMP \u53d7\u898f\u7bc4\u88fd\u85e5\u54c1\u7ba1\u5be6\u9a57\u5ba4\u7684\u5178\u578b\u6642\u7a0b\uff1b\u4f9d\u4f60\u7684\u74b0\u5883\u8abf\u6574\u3002<\/p>\n<h3>\u6bcf\u65e5\uff08\u7cfb\u7d71\u9069\u7528\u6027\uff09<\/h3>\n<ul>\n<li>\u5728\u5df2\u77e5\u6fc3\u5ea6\u8dd1\u4e00\u500b\u54c1\u7ba1\u6a19\u6e96\u3002\u63a5\u53d7\u6a19\u6e96\uff1a\u91cf\u5230\u7684\u503c\u5728\u9810\u671f\u7684 \u00b12% \u5167\u3002\u9019\u662f\u500b\u55ae\u9ede\u6aa2\u67e5\uff0c\u6293\u7c97\u5927\u7684\u5931\u6548\uff08\u71c8\u6ec5\u3001\u6bd4\u8272\u76bf\u63d2\u932f\u69fd\u3001\u5100\u5668\u96e2\u7dda\uff09\u3002<\/li>\n<li>\u5728\u6aaf\u71c8\u4e0b\u76ee\u8996\u6aa2\u67e5\u6bd4\u8272\u76bf\u3002\u7121\u53ef\u898b\u522e\u75d5\u3001\u8584\u819c\u6216\u6c59\u67d3\u3002<\/li>\n<li>\u9996\u6b21\u91cf\u6e2c\u524d\u71c8\u9810\u71b1\u2014\u2014\u6c18\u71c8\u6700\u5c11 30 \u5206\u9418\uff1b\u4e5f\u8b93\u93a2\u71c8\u7a69\u5b9a\u4e0b\u4f86\u3002<\/li>\n<\/ul>\n<h3>\u6bcf\u9031<\/h3>\n<ul>\n<li>\u7528\u9225\u73bb\u7483\u56fa\u9ad4\u53c3\u8003\u505a\u6ce2\u9577\u5feb\u67e5\u3002\u6383 240\u2013650 nm\uff1b\u9a57\u8b49\u4e09\u5230\u56db\u500b\u7279\u5fb5\u5cf0\u5728\u5bb9\u5dee\u5167\u3002<\/li>\n<li>\u6bd4\u8272\u76bf\u914d\u5c0d\u5339\u914d\u6aa2\u67e5\uff08\u96d9\u5149\u675f\u5100\u5668\uff09\u3002\u5169\u652f\u6bd4\u8272\u76bf\u90fd\u586b\u53bb\u96e2\u5b50\u6c34\u6216\u5de5\u4f5c\u7a7a\u767d\uff1b\u57fa\u7dda\u6383\u63cf\u61c9\u662f\u8de8 200\u2013800 nm \u7684 0.000 \u00b1 0.005 AU\u3002<\/li>\n<\/ul>\n<h3>\u6bcf\u6708<\/h3>\n<ul>\n<li>\u4f9d USP <851> \u7528\u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2\u505a\u5b8c\u6574\u6ce2\u9577\u6e96\u78ba\u5ea6\u9a57\u8b49\u3002\u5168\u90e8\u5341\u4e09\u500b\u7279\u5fb5\u5cf0\uff1b\u901a\u904e\u6a19\u6e96 \u00b11 nm \u7d2b\u5916 \/ \u00b13 nm \u53ef\u898b\u3002<\/li>\n<li>\u7528 K\u2082Cr\u2082O\u2087 \u53c3\u8003\u5728 257 nm \u505a\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u3002\u901a\u904e\u6a19\u6e96\u76f8\u5c0d\u8a8d\u8b49 A\u00b9% \u7684 \u00b12%\u3002<\/li>\n<li>\u7528 198 nm \u7684 1.2% KCl \u8207 220 nm \u7684 1% NaI \u505a\u96dc\u6563\u5149\u6aa2\u67e5\u3002\u901a\u904e\u6a19\u6e96\u91cf\u5230 A > 1.5 AU\u3002<\/li>\n<\/ul>\n<h3>\u6bcf\u5b63<\/h3>\n<ul>\n<li>\u7528 6\u20138 \u500b\u6fc3\u5ea6\u7684 K\u2082Cr\u2082O\u2087 \u7a00\u91cb\u7cfb\u5217\u505a\u5149\u5ea6\u7dda\u6027\u3002\u901a\u904e\u6a19\u6e96\u8de8\u5de5\u4f5c AU \u7bc4\u570d r\u00b2 \u2265 0.999\u3002<\/li>\n<li>\u7528\u5df1\u70f7\u4e2d 0.020% \u7532\u82ef\u505a\u5149\u8b5c\u89e3\u6790\u5ea6\u3002\u901a\u904e\u6a19\u6e96 A269 \/ A266 \u2265 1.5\u3002<\/li>\n<li>\u6bd4\u8272\u76bf\u5149\u7a0b\u9a57\u8b49\u2014\u2014\u7528 K\u2082Cr\u2082O\u2087 \u505a\u6717\u4f2f-\u6bd4\u723e\uff0c\u6216\u5c0d\u53c3\u8003\u6bd4\u8272\u76bf\u505a\u5dee\u5206\u6bd4\u8f03\u3002<\/li>\n<\/ul>\n<h3>\u6bcf\u5e74<\/h3>\n<ul>\n<li>\u7531\u5149\u8b5c\u5100\u4f9b\u61c9\u5546\u6216\u8a8d\u8b49\u7684\u7b2c\u4e09\u65b9\u7dad\u4fee\u505a\u5b8c\u6574\u7684\u5b89\u88dd\u9a57\u8b49 \/ \u64cd\u4f5c\u9a57\u8b49\uff08IQ\/OQ\uff09\u3002\u5728\u984d\u5b9a\u58fd\u547d\u7d42\u7d50\u6642\u63db\u71c8\uff08D\u2082 ~ 2000 \u5c0f\u6642\u3001\u93a2\u71c8 ~ 5000 \u5c0f\u6642\uff09\u3002<\/li>\n<li>\u5c0d\u7576\u524d\u5df2\u767c\u8868\u7684\u85e5\u5178\u65b9\u6cd5\u7248\u672c\u5be9\u6838 SOP\u2014\u2014\u65b9\u6cd5\u6703\u9031\u671f\u6027\u66f4\u65b0\u3001\u4f60\u7684 SOP \u61c9\u8a72\u8ddf\u4e0a\u3002<\/li>\n<li>\u5728\u4f8b\u884c\u4f7f\u7528\u597d\u5e7e\u5e74\u5f8c\u91cd\u65b0\u767c\u884c\u6bd4\u8272\u76bf CoA\uff0c\u6216\u5982\u679c\u5de5\u4f5c\u7d44\u5df2\u7d2f\u7a4d\u53ef\u898b\u78e8\u640d\u5c31\u63db\u5230\u4e00\u7d44\u65b0\u7684\u6bd4\u8272\u76bf\u3002<\/li>\n<\/ul><h2 id=\"documentation\">11. \u6587\u4ef6\u8207 SOP \u8981\u6c42<\/h2>\n<p>\u9a57\u8b49\u53ea\u6709\u5728\u6709\u6587\u4ef6\u8a18\u8f09\u6642\u624d\u7b97\u6578\u3002\u7a3d\u6838\u54e1\u5728\u78ba\u6548\u6a94\u6848\u88e1\u770b\u4e09\u4ef6\u4e8b\uff1aSOP \u672c\u8eab\uff08\u754c\u5b9a\u9a57\u8b49\u4ec0\u9ebc\u3001\u7528\u4ec0\u9ebc\u53c3\u8003\u3001\u4ec0\u9ebc\u983b\u7387\u3001\u4ec0\u9ebc\u5bb9\u5dee\uff09\u3001\u9a57\u8b49\u7d00\u9304\uff08\u6bcf\u500b\u9a57\u8b49\u6d3b\u52d5\u6709\u7c3d\u540d\u8207\u65e5\u671f\u7684\u7d00\u9304\uff09\uff0c\u8207\u8da8\u52e2\u5206\u6790\uff08\u6b77\u53f2\u8868\u73fe\uff0c\u5728\u6f02\u79fb\u8d8a\u904e\u5bb9\u5dee\u4e4b\u524d\u8fa8\u8b58\u51fa\u4f86\uff09\u3002<\/p>\n<h3>SOP \u6700\u4f4e\u5167\u5bb9<\/h3>\n<ul>\n<li>\u53c3\u7167\u9069\u7528\u7684\u85e5\u5178\u65b9\u6cd5\uff08USP <851>\u3001EP 2.2.25 \u7b49\uff09\u542b\u7248\u672c\u65e5\u671f<\/li>\n<li>\u5217\u51fa\u9a57\u8b49\u6d3b\u52d5\u53ca\u983b\u7387\uff08\u6bcf\u65e5 \/ \u6bcf\u9031 \/ \u6bcf\u6708 \/ \u6bcf\u5b63 \/ \u6bcf\u5e74\uff09<\/li>\n<li>\u53c3\u8003\u7269\u8cea\u542b NIST \u6216\u85e5\u5178\u5f59\u7de8\u4f86\u6e90\u3001\u6279\u865f\u8207\u6709\u6548\u671f<\/li>\n<li>\u6bcf\u500b\u6e2c\u8a66\u7684\u63a5\u53d7\u6a19\u6e96<\/li>\n<li>\u5931\u6557\u6642\u600e\u9ebc\u505a\uff08\u91cd\u67e5\u3001\u5347\u7d1a\u3001\u8b93\u5100\u5668\u96e2\u7dda\u3001\u806f\u7d61\u4f9b\u61c9\u5546\uff09<\/li>\n<li>\u6bcf\u500b\u6e2c\u8a66\u7684\u6587\u4ef6\u8981\u6c42\uff08\u64cd\u4f5c\u8005\u7e2e\u5beb\u3001\u65e5\u671f\u3001\u539f\u59cb\u6578\u64da\u6a94\u3001\u8a08\u7b97\u7d50\u679c\u3001\u901a\u904e\/\u5931\u6557\uff09<\/li>\n<li>SOP \u672c\u8eab\u7684\u9031\u671f\u6027\u5be9\u6838\u6642\u7a0b<\/li>\n<\/ul>\n<h3>\u9a57\u8b49\u7d00\u9304\u6700\u4f4e\u5167\u5bb9<\/h3>\n<ul>\n<li>\u65e5\u671f\u8207\u6642\u9593<\/li>\n<li>\u64cd\u4f5c\u8005\uff08\u7e2e\u5beb\u6216\u4f7f\u7528\u8005\u540d\u7a31\uff09<\/li>\n<li>\u5100\u5668\u8b58\u5225\u78bc\uff08\u5e8f\u865f\uff09<\/li>\n<li>\u53c3\u8003\u7269\u8cea\u8b58\u5225\u78bc\uff08NIST SRM \u7de8\u865f\u3001\u6279\u865f\u3001\u6709\u6548\u671f\uff09<\/li>\n<li>\u539f\u59cb\u91cf\u6e2c\u6578\u64da\uff08\u5132\u5b58\u7684\u5149\u8b5c\u6a94\u6216\u6578\u503c\uff09<\/li>\n<li>\u8a08\u7b97\u7d50\u679c\uff08\u5cf0\u4f4d\u7f6e\u3001\u6bd4\u503c\u3001\u5438\u5149\u5ea6\u503c\uff09<\/li>\n<li>\u63a5\u53d7\u6a19\u6e96<\/li>\n<li>\u901a\u904e \/ \u5931\u6557\u7d50\u8ad6<\/li>\n<li>\u64cd\u4f5c\u8005\u7c3d\u540d\uff1b\u5be9\u6838\u8005\u7c3d\u540d<\/li>\n<\/ul>\n\n<div class=\"csg-callout protocol\"><strong>\u8da8\u52e2\u5206\u6790\u662f\u6293\u6162\u6f02\u79fb\u7684\u6771\u897f\u3002<\/strong> \u628a\u904e\u53bb 12 \u500b\u6708\u7684\u6ce2\u9577\u6e96\u78ba\u5ea6\u3001257 nm \u5438\u5149\u5ea6\u8207\u96dc\u6563\u5149\u4f5c\u5716\u3002\u4e00\u53f0\u5728\u7b2c 9 \u500b\u6708\u5e95\u8da8\u5411\u5bb9\u5dee\u6975\u9650\u7684\u5149\u8b5c\u5100\uff0c\u662f\u4f60\u8a72\u5728\u7b2c 10 \u500b\u6708\u3001\u800c\u4e0d\u662f\u5b83\u771f\u6b63\u5931\u6557\u7684\u7b2c 12 \u500b\u6708\u63d0\u51fa\u7684\u4f9b\u61c9\u5546\u7dad\u4fee\u8acb\u6c42\u3002\u591a\u6578 LIMS \u7cfb\u7d71\u652f\u63f4\u81ea\u52d5\u8da8\u52e2\u5831\u544a\uff1b\u5982\u679c\u4f60\u7684\u4e0d\u652f\u63f4\uff0c\u6bcf\u5b63\u4e00\u6b21\u7684 Excel \u5be9\u6838\u82b1 15 \u5206\u9418\u3001\u5c31\u80fd\u907f\u514d OOS \u8abf\u67e5\u3002<\/div><h2 id=\"standards\">12. \u9078\u64c7\u53c3\u8003\u7269\u8cea<\/h2>\n<p>\u85e5\u5178\u65b9\u6cd5\u63a5\u53d7\u597d\u5e7e\u7a2e\u53c3\u8003\u7269\u8cea\u985e\u5225\uff0c\u5404\u6709\u4e0d\u540c\u7684\u6210\u672c\u3001\u53ef\u8ffd\u6eaf\u6027\u8207\u4fbf\u5229\u6027\u53d6\u6368\u3002<\/p>\n<h3>NIST SRM\uff08\u9ec3\u91d1\u6a19\u6e96\uff09<\/h3>\n<table>\n<thead><tr><th>SRM<\/th><th>\u7269\u8cea<\/th><th>\u9a57\u8b49<\/th><th>\u5178\u578b\u6210\u672c<\/th><\/tr><\/thead>\n<tbody>\n<tr><td>SRM 2034<\/td><td>HClO\u2084 \u4e2d\u7684\u9225\u6c27\u5316\u7269\u6eb6\u6db2<\/td><td>\u6ce2\u9577\u6e96\u78ba\u5ea6<\/td><td>$$$<\/td><\/tr>\n<tr><td>SRM 935a<\/td><td>\u91cd\u927b\u9178\u9240\uff08\u9ad8\u7d14\u5ea6\uff09<\/td><td>\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u8207\u7dda\u6027<\/td><td>$$<\/td><\/tr>\n<tr><td>SRM 930e<\/td><td>\u4e2d\u6027\u5bc6\u5ea6\u73bb\u7483\u6ffe\u5149\u7247\u7d44<\/td><td>\u56fa\u5b9a AU \u503c\u7684\u5438\u5149\u5ea6<\/td><td>$$$$<\/td><\/tr>\n<tr><td>SRM 1930<\/td><td>\u4e09\u7247\u8a8d\u8b49 \u03bb \u7684\u6ffe\u5149\u7247<\/td><td>\u6ce2\u9577 + \u5438\u5149\u5ea6\u5408\u4f75<\/td><td>$$$$<\/td><\/tr>\n<tr><td>SRM 2032<\/td><td>HClO\u2084 \u4e2d\u7684 KIO\u2083\uff08\u96dc\u6563\u5149\uff09<\/td><td>\u7d2b\u5916\u96dc\u6563\u5149<\/td><td>$$$<\/td><\/tr>\n<tr><td>SRM 2031<\/td><td>\u77f3\u82f1\u4e0a\u934d\u91d1\u5c6c\u7684\u4e2d\u6027\u5bc6\u5ea6<\/td><td>\u9ad8 AU \u5438\u5149\u5ea6<\/td><td>$$$$$<\/td><\/tr>\n<\/tbody>\n<\/table>\n<h3>\u5546\u696d\u66ff\u4ee3\uff08NIST \u53ef\u8ffd\u6eaf\uff09<\/h3>\n<p>\u597d\u5e7e\u5bb6\u4f9b\u61c9\u5546\u4ee5\u6bd4\u76f4\u63a5\u8cb7 SRM \u66f4\u4f4e\u7684\u6210\u672c\u4f9b\u61c9 NIST \u53ef\u8ffd\u6eaf\u7684\u53c3\u8003\u7269\u8cea\uff1aStarna Cells\uff08\u82f1\u570b\uff09\u3001Hellma Analytics\uff08\u5fb7\u570b\uff09\u3001Reagecon\uff08\u611b\u723e\u862d\uff09\u3001Camspec\uff08\u82f1\u570b\uff09\u3002\u5b83\u5011\u7684\u8b49\u66f8\u900f\u904e\u5c0d SRM \u7269\u8cea\u7684\u9031\u671f\u6027\u6821\u6b63\u5e36\u6709 NIST \u53ef\u8ffd\u6eaf\u6027\u3002\u5c0d\u591a\u6578\u88fd\u85e5\u54c1\u7ba1\u53ef\u63a5\u53d7\uff1b\u78ba\u8a8d\u4f60\u7684\u7a3d\u6838\u54e1\u63a5\u53d7\u9019\u500b\u8ffd\u6eaf\u93c8\u3002<\/p>\n<h3>\u56fa\u9ad4 vs \u6db2\u9ad4\u53c3\u8003\u7269\u8cea<\/h3>\n<ul>\n<li><strong>\u56fa\u9ad4\uff08\u9225\u73bb\u7483\u3001\u91f9\u9420\u73bb\u7483\u3001NIST \u73bb\u7483\u6ffe\u5149\u7247\uff09\uff1a<\/strong> \u4fdd\u5b58\u671f\u9577\uff0810+ \u5e74\uff09\u3001\u4e0d\u9700\u88fd\u5099\u3001\u5c0d\u4f8b\u884c\u5feb\u67e5\u65b9\u4fbf\u3002\u76f8\u5c0d\u6db2\u9ad4\u6eb6\u6db2\u6709\u8f15\u5fae\u7684\u5cf0\u5c55\u5bec\u3002<\/li>\n<li><strong>\u6db2\u9ad4\uff08\u9225\u904e\u6c2f\u9178\u3001K\u2082Cr\u2082O\u2087\uff09\uff1a<\/strong> \u85e5\u5178\u7684\u4e3b\u8981\u53c3\u8003\uff1b\u5cf0\u66f4\u92b3\u5229\uff1b\u8655\u7406\u4e0a\u66f4\u8b1b\u7a76\uff08\u5bc6\u5c01\u6bd4\u8272\u76bf\u3001\u9031\u671f\u6027\u91cd\u65b0\u88fd\u5099\u30011\u20135 \u5e74\u4fdd\u5b58\u671f\uff09\u3002<\/li>\n<\/ul>\n<h3>\u81ea\u884c\u88fd\u5099<\/h3>\n<p>\u9225\u904e\u6c2f\u9178\u8207 K\u2082Cr\u2082O\u2087 \u53c3\u8003\u6eb6\u6db2\u90fd\u80fd\u4f9d\u5df2\u767c\u8868\u7684 USP \u65b9\u6cd5\u3001\u7528 NIST \u7d1a\u6216 USP \u7d1a\u5316\u5b78\u54c1\u81ea\u884c\u88fd\u5099\u3002\u82e5\u53ef\u8ffd\u6eaf\u5230\u8a8d\u8b49\u7684\u6bcd\u9ad4\u7269\u8cea\uff0c\u81ea\u884c\u88fd\u5099\u5c0d\u9a57\u8b49\u5de5\u4f5c\u53ef\u63a5\u53d7\u3002\u8a18\u9304\u88fd\u5099\u3001\u5728\u78ba\u6548\u7684\u5b89\u5b9a\u6027\u7a97\u53e3\u5167\u4f7f\u7528\uff0c\u4e26\u5728\u4f7f\u7528\u524d\u9a57\u8b49\u88fd\u5099\u6eb6\u6db2\u7684\u5149\u8b5c\u7b26\u5408\u5df2\u767c\u8868\u503c\u3002<\/p>\n\n<div class=\"csg-callout\"><strong>\u6211\u5011\u505a\u4ec0\u9ebc\u3001\u4e0d\u505a\u4ec0\u9ebc\u3002<\/strong> MachinedQuartz \u88fd\u9020\u7528\u4f86\u88dd\u9019\u4e9b\u53c3\u8003\u7269\u8cea\u7684\u6a19\u6e96 10 mm \u8207\u914d\u5c0d\u6bd4\u8272\u76bf\uff0c\u4e26\u9644\u4e0a\u8f09\u660e\u6bd4\u8272\u76bf\u5149\u7a0b\u5230\u4f60\u898f\u683c\u7684 CoA\u3002\u6211\u5011\u76ee\u524d\u4e0d\u51fa\u8ca8\u53c3\u8003\u6eb6\u6db2\u6216\u56fa\u9ad4\u53c3\u8003\u73bb\u7483\uff1b\u90a3\u4e9b\u7684\u63a1\u8cfc\u662f\u5c0d\u4e0a\u9762\u90a3\u4e9b\u4f9b\u61c9\u5546\u7684\u53e6\u4e00\u500b\u6c7a\u5b9a\u3002<\/div><h2 id=\"tools\">13. \u5de5\u5177\u3001\u76f8\u95dc\u6307\u5357\u8207\u6bd4\u8272\u76bf\u578b\u9304<\/h2>\n<p>\u4e0b\u9762\u7684\u9801\u9762\u5b8c\u6210\u9a57\u8b49\u5de5\u5177\u93c8\uff1a\u88dd\u53c3\u8003\u7269\u8cea\u7684\u6bd4\u8272\u76bf\u3001\u5149\u7a0b\u9a57\u8b49\u8a08\u7b97\u7684\u8a08\u7b97\u5668\uff0c\u4ee5\u53ca\u5f71\u97ff\u65e5\u5e38\u91cf\u6e2c\u53ef\u9760\u6027\u7684\u6bd4\u8272\u76bf\u9078\u578b\u8207\u6e05\u6f54\u76f8\u95dc\u6307\u5357\u3002<\/p>\n\n<div class=\"csg-cta-box\"><h3>\u9700\u8981\u6709\u6587\u4ef6\u8a18\u8f09 CoA \u7684\u6bd4\u8272\u76bf\uff1f<\/h3><p>\u6240\u6709 MachinedQuartz \u6bd4\u8272\u76bf\u90fd\u53ef\u9644\u4e0a\u53ef\u8ffd\u6eaf\u5230\u81ea\u6709\u6821\u6b63\u6a19\u6e96\u7684\u5149\u7a0b CoA\u3002\u914d\u5c0d\u7d44\u8207\u85e5\u5178\u7d1a\u6587\u4ef6\uff0c\u5e36\u8457\u4f60\u7684\u898f\u683c\u806f\u7d61\u6211\u5011\u3002<\/p><a href=\"https:\/\/machinedquartz.com\/contact\/\" class=\"csg-cta-btn\">\u7d22\u53d6 CoA \u5831\u50f9 \u2192<\/a><a href=\"https:\/\/machinedquartz.com\/bulk-quartz-cuvettes\/\" class=\"csg-cta-btn outline\">\u6279\u91cf\u8207 OEM \u65b9\u6848 \u2192<\/a><\/div>\n\n<div class=\"csg-related-grid\">\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/cuvette-path-length-by-analyte\/\"><span class=\"csg-related-icon\">\ud83d\udcd0<\/span><span class=\"csg-related-h\">\u4f9d\u5f85\u6e2c\u7269\u5206\u7684\u5149\u7a0b<\/span><span class=\"csg-related-d\">\u4e00\u65e6\u9a57\u8b49\u78ba\u8a8d\u6bd4\u8272\u76bf\u5c31\u662f\u5176\u6a19\u7c64\u8aaa\u7684\uff0c\u6311\u5c0d\u7684\u5149\u7a0b\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/path-length-calculator\/\"><span class=\"csg-related-icon\">\ud83d\udccb<\/span><span class=\"csg-related-h\">\u6717\u4f2f-\u6bd4\u723e\u8a08\u7b97\u5668<\/span><span class=\"csg-related-d\">\u4f9b\u5149\u7a0b\u9a57\u8b49\u8a08\u7b97\uff1aA \/ (\u03b5 \u00b7 c) = l\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/uv-vis-troubleshooting-guide\/\"><span class=\"csg-related-icon\">\ud83d\udd27<\/span><span class=\"csg-related-h\">UV-Vis \u7591\u96e3\u6392\u89e3<\/span><span class=\"csg-related-d\">\u8a3a\u65b7\u6c23\u6ce1\u3001\u689d\u7d0b\u3001\u57fa\u7dda\u6f02\u79fb\u2014\u2014\u9a57\u8b49\u5931\u6557\u7684\u75c7\u72c0\u9762\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/cuvette-cleaning-protocol\/\"><span class=\"csg-related-icon\">\ud83e\uddec<\/span><span class=\"csg-related-h\">\u6e05\u6f54\u898f\u7a0b<\/span><span class=\"csg-related-d\">\u5728\u91cf\u6e2c\u4e4b\u9593\u8b93\u9a57\u8b49\u7d1a\u6bd4\u8272\u76bf\u4fdd\u6301\u7121\u6c59\u67d3\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/zh-hant\/quartz-cuvette-uv-cutoff\/\"><span class=\"csg-related-icon\">\ud83d\udd2c<\/span><span class=\"csg-related-h\">\u4f9d\u7b49\u7d1a\u5206\u7684\u7d2b\u5916\u622a\u6b62<\/span><span class=\"csg-related-d\">JGS1 \/ JGS2 \/ JGS3 \u900f\u5c04\u66f2\u7dda\u2014\u2014\u70ba\u7d2b\u5916\u9a57\u8b49\u9078\u6bd4\u8272\u76bf\u6750\u8cea\u3002<\/span><\/a>\n<a class=\"csg-related-card\" href=\"https:\/\/machinedquartz.com\/bulk-quartz-cuvettes\/\"><span class=\"csg-related-icon\">\ud83d\udce6<\/span><span class=\"csg-related-h\">\u6279\u91cf\u8207 OEM<\/span><span class=\"csg-related-d\">\u91cf\u8a02\u3001\u914d\u5c0d\u7d44\u3001\u5b8c\u6574\u54c1\u7ba1\u6587\u4ef6\u5305\u3002<\/span><\/a>\n<\/div><h2 id=\"faq\">14. \u5e38\u898b\u554f\u984c<\/h2>\n<div class=\"csg-faq\">\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u4ec0\u9ebc\u662f USP <851> \u3001\u70ba\u4f55\u5b83\u91cd\u8981\uff1f<\/div><div class=\"csg-faq-a\"><p>USP <851> \u5206\u5149\u5149\u5ea6\u6cd5\u8207\u5149\u6563\u5c04\u662f\u7f8e\u570b\u85e5\u5178\u88e1\u7684\u7ae0\u7bc0\uff0c\u754c\u5b9a\u9a57\u8b49\u7528\u65bc\u88fd\u85e5\u5206\u6790\u7684 UV-Vis \u5206\u5149\u5149\u5ea6\u8a08\u7684\u65b9\u6cd5\u8207\u63a5\u53d7\u6a19\u6e96\u3002\u5b83\u898f\u5b9a\u6ce2\u9577\u6e96\u78ba\u5ea6\u6e2c\u8a66\uff08\u7528\u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2\uff09\u3001\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u6e2c\u8a66\uff08\u7528\u91cd\u927b\u9178\u9240\uff09\u3001\u5149\u5ea6\u7dda\u6027\u3001\u96dc\u6563\u5149\u9650\u503c\u8207\u89e3\u6790\u5ea6\u3002\u5728\u7f8e\u570b\uff0c\u4efb\u4f55\u7528\u65bc GMP \u53d7\u898f\u7bc4\u88fd\u85e5\u5206\u6790\u7684\u5206\u5149\u5149\u5ea6\u8a08\u90fd\u9700\u8981\u5408\u898f\u3002EP 2.2.25\u3001JP 2.24 \u8207 ChP 0401 \u662f\u6b50\u6d32\u3001\u65e5\u672c\u8207\u4e2d\u570b\u7684\u7b49\u6548\u65b9\u6cd5\u3001\u672c\u8cea\u4e0a\u5354\u8abf\u4e00\u81f4\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u505a GMP \u5de5\u4f5c\u6211\u591a\u4e45\u8981\u9a57\u8b49\u4e00\u6b21\u5206\u5149\u5149\u5ea6\u8a08\uff1f<\/div><div class=\"csg-faq-a\"><p>\u983b\u7387\u7531\u5be6\u9a57\u5ba4\u7684\u54c1\u8cea\u7cfb\u7d71\u8207\u91cf\u6e2c\u7684\u95dc\u9375\u6027\u6c7a\u5b9a\u3002\u5178\u578b GMP \u6642\u7a0b\uff1a\u6bcf\u65e5\u7528\u55ae\u9ede\u54c1\u7ba1\u6a19\u6e96\u505a\u7cfb\u7d71\u9069\u7528\u6027\uff1b\u6bcf\u9031\u7528\u9225\u73bb\u7483\u505a\u6ce2\u9577\u5feb\u67e5\uff1b\u6bcf\u6708\u7528 USP \u53c3\u8003\u6eb6\u6db2\u505a\u5b8c\u6574\u6ce2\u9577\u8207\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\uff1b\u6bcf\u5b63\u505a\u5149\u5ea6\u7dda\u6027\u8207\u89e3\u6790\u5ea6\uff1b\u6bcf\u5e74\u7531\u4f9b\u61c9\u5546\u6216\u7b2c\u4e09\u65b9\u7dad\u4fee\u505a\u5b8c\u6574 IQ\/OQ\u3002\u4f9d\u4f60\u5177\u9ad4\u7684\u54c1\u8cea\u7cfb\u7d71\u8981\u6c42\u8207\u5100\u5668\u5931\u6548\u53f2\u8abf\u6574\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u80fd\u7528\u9225\u73bb\u7483\u53d6\u4ee3\u9225\u6c27\u5316\u7269\u6eb6\u6db2\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u4f8b\u884c\u7684\u4e2d\u9593\u6aa2\u67e5\u53ef\u4ee5\u3002USP <851> \u5169\u8005\u90fd\u8a8d\u53ef\u3002\u56fa\u9ad4\u9225\u73bb\u7483\u5c0d\u6bcf\u65e5\u6216\u6bcf\u9031\u5feb\u67e5\u65b9\u4fbf\uff0c\u56e0\u70ba\u4e0d\u9700\u88fd\u5099\u6eb6\u6db2\u3002\u5b8c\u6574\u85e5\u5178\u78ba\u6548\uff08\u6bcf\u6708\u6216\u6bcf\u5e74\u7684\u5b8c\u6574\u9a57\u8b49\uff09\uff0c\u6db2\u9ad4\u9225\u6c27\u5316\u7269\u904e\u6c2f\u9178\u6eb6\u6db2\u662f\u4e3b\u8981\u53c3\u8003\uff0c\u56e0\u70ba\u5cf0\u4f4d\u7f6e\u66f4\u92b3\u5229\u3001\u8a8d\u8b49\u503c\u900f\u904e SRM 2034 \u53ef\u8ffd\u6eaf\u5230 NIST\u3002\u5169\u8005\u90fd\u63a5\u53d7\uff1b\u56fa\u9ad4\u8f03\u5feb\u3001\u6db2\u9ad4\u8f03\u56b4\u8b39\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u4f9d USP \u6ce2\u9577\u6e96\u78ba\u5ea6\u5bb9\u5dee\u662f\u591a\u5c11 <851>\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5728\u7b49\u65bc\u6216\u4f4e\u65bc 400 nm \u7684\u6ce2\u9577\uff08\u7d2b\u5916\u5340\uff09\u6b63\u8ca0 1 nm\uff0c\u5728\u9ad8\u65bc 400 nm \u7684\u6ce2\u9577\uff08\u53ef\u898b\u5340\uff09\u6b63\u8ca0 3 nm\u3002EP 2.2.25 \u7528\u985e\u4f3c\u7684\u5bb9\u5dee\u3002\u8981\u66f4\u56b4\u7684\u6ce2\u9577\u63a7\u5236\uff080.5 nm \u4ee5\u4e0b\uff09\uff0c\u9700\u8981\u5c08\u9580\u7684\u7814\u7a76\u5100\u5668\u8207\u65b9\u6cd5\uff0cSOP \u61c9\u898f\u5b9a\u66f4\u56b4\u7684\u5bb9\u5dee\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u9700\u8981\u9a57\u8b49\u6bd4\u8272\u76bf\u5149\u7a0b\u55ce\uff0c\u9084\u662f\u88fd\u9020\u5546 CoA \u5c31\u5920\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5c0d\u9032\u6599\u6aa2\u9a57\u8207\u4f8b\u884c\u4f7f\u7528\uff0c\u53ea\u8981\u88fd\u9020\u5546 CoA \u8f09\u660e\u5149\u7a0b\u5728\u6b63\u8ca0 0.005 cm \u5bb9\u5dee\u5167\u3001\u4e14\u53ef\u8ffd\u6eaf\u5230\u6821\u6b63\u6a19\u6e96\uff0c\u5c31\u53ef\u63a5\u53d7\u3002\u5c0d\u9577\u671f\u4f7f\u7528\u4e2d\uff08GMP \u6bd4\u8272\u76bf\u901a\u5e38\u6bcf 1 \u5230 2 \u5e74\uff09\uff0c\u7528 K2Cr2O7 \u53c3\u8003\u6eb6\u6db2\u505a\u6717\u4f2f-\u6bd4\u723e\u7684\u81ea\u884c\u9a57\u8b49\uff0c\u6293\u5f97\u5230\u539f\u59cb CoA \u6355\u6349\u4e0d\u5230\u7684\u4f7f\u7528\u4e2d\u6f02\u79fb\u3002\u85e5\u5178\u5de5\u4f5c\uff0c\u5728 SOP \u88e1\u8a18\u9304\u9a57\u8b49\u505a\u6cd5\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u96d9\u5149\u675f\u5206\u5149\u5149\u5ea6\u8a08\u6211\u600e\u9ebc\u914d\u5c0d\u6bd4\u8272\u76bf\uff1f<\/div><div class=\"csg-faq-a\"><p>\u5169\u652f\u6bd4\u8272\u76bf\u90fd\u586b\u540c\u6a23\u7684\u7a7a\u767d\u6eb6\u5291\u3002\u4e00\u652f\u653e\u6a23\u54c1\u5149\u675f\u3001\u4e00\u652f\u653e\u53c3\u8003\u5149\u675f\u3002\u5f9e 200 \u5230 800 nm \u8dd1\u57fa\u7dda\u6383\u63cf\u3002\u91cf\u5230\u7684\u5438\u5149\u5ea6\u5728\u6bcf\u500b\u6ce2\u9577\u90fd\u61c9\u662f 0.000 \u6b63\u8ca0 0.005 AU\u3002\u4efb\u4f55\u4e00\u81f4\u7684\u504f\u5dee\u5c31\u662f\u914d\u5c0d\u4e0d\u5339\u914d\u3002\u53ef\u63a5\u53d7\u7684\u914d\u5c0d\u5728\u5de5\u4f5c\u5149\u8b5c\u4e0a delta-A \u5c0f\u65bc 0.005\u3002\u6bd4\u8272\u76bf\u88fd\u9020\u5546\u7684\u9810\u5339\u914d\u914d\u5c0d\u9644\u6709\u6587\u4ef6\u8a18\u8f09\u7684\u4e0d\u5339\u914d\u8f2a\u5ed3\u3001\u662f\u6700\u7c21\u55ae\u7684\u9014\u5f91\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u91cd\u927b\u9178\u9240\u5728 257 nm \u7684 A1% \u503c\u662f\u591a\u5c11\uff1f<\/div><div class=\"csg-faq-a\"><p>5 mM H2SO4 \u4e2d K2Cr2O7 \u5728 257 nm \u7684 A1%\uff081% w\/v \u6eb6\u6db2\u5728 10 mm \u6bd4\u8272\u76bf\u88e1\u7684\u5438\u5149\u5ea6\uff09\u4f9d USP \u662f 144.0 <851>\u5728 235 nm \u662f 124.5\u3002\u5728 313 nm\uff0c48.6\u3002\u5728 350 nm\uff0c106.6\u3002\u9019\u4e9b\u662f\u5be6\u9a57\u91cf\u6e2c\u5fc5\u9808\u5728\u76f8\u5c0d\u6b63\u8ca0 2% \u5167\u7b26\u5408\u3001\u624d\u80fd\u901a\u904e\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u9a57\u8b49\u7684\u8a8d\u8b49\u503c\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u7684\u5149\u8b5c\u5100\u6c92\u901a\u904e\u7532\u82ef\/\u5df1\u70f7\u89e3\u6790\u5ea6\u6e2c\u8a66\u662f\u4ec0\u9ebc\u610f\u601d\uff1f<\/div><div class=\"csg-faq-a\"><p>\u610f\u601d\u662f\u4f60\u55ae\u8272\u5668\u7684\u5149\u8b5c\u983b\u5bec\uff08SBW\uff09\u592a\u5bec\uff0c\u7121\u6cd5\u5206\u8fa8\u5728 269 \u8207 266 nm \u7dca\u9130\u7684\u7532\u82ef\u5cf0\u3002A269\/A266 \u6bd4\u503c\u8dcc\u5230 USP <851> \u901a\u904e\u6a19\u6e96 1.5 \u4ee5\u4e0b\u3002\u4e0d\u662f\u5149\u8b5c\u5100\u4ee5\u592a\u5bec\u7684 SBW \u8a2d\u5b9a\u64cd\u4f5c\uff08\u5982\u679c\u5100\u5668\u5141\u8a31\u5c31\u964d\u4f4e SBW\uff09\uff0c\u5c31\u662f\u5100\u5668\u7684\u8a2d\u8a08 SBW \u5c0d\u53d7\u898f\u7bc4\u65b9\u6cd5\u592a\u5bec\uff08\u5100\u5668\u53ef\u80fd\u4e0d\u9069\u5408\u9019\u985e\u5de5\u4f5c\uff09\u3002\u5c0d\u591a\u6578\u5cf0\u8f03\u5bec\u7684\u88fd\u85e5\u6e2c\u5b9a\uff0c\u9019\u5f88\u5c11\u662f\u9650\u5236\u56e0\u7d20\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u6211\u80fd\u81ea\u884c\u88fd\u5099\u9225\u6c27\u5316\u7269\u6eb6\u6db2\u55ce\uff1f<\/div><div class=\"csg-faq-a\"><p>\u53ef\u4ee5\u3002USP <851> \u65b9\u6cd5\u898f\u5b9a\u88fd\u5099\uff1a4 g \u9ad8\u7d14\u5ea6 Ho2O3 \u6eb6\u65bc 100 mL \u7684 1.4 M HClO4\u3002\u88fd\u5099\u597d\u7684\u6eb6\u6db2\u61c9\u586b\u5165\u5e36\u585e\u7684 10 mm \u77f3\u82f1\u6bd4\u8272\u76bf\u3001\u5728\u4f7f\u7528\u524d\u5c0d\u5df2\u767c\u8868\u7684\u5cf0\u4f4d\u7f6e\u9a57\u8b49\u3002\u81ea\u884c\u88fd\u5099\u9700\u8981\u9ad8\u7d14\u5ea6\uff08\u5927\u65bc 99.9%\uff09\u9225\u6c27\u5316\u7269\u8207\u6fc3\u904e\u6c2f\u9178\uff1b\u5728 SOP \u88e1\u8a18\u9304\u88fd\u5099\u3001\u6709\u6548\u671f\u8207\u9a57\u8b49\u3002\u5982\u679c\u4f60\u60f3\u8981\u6bcd\u9ad4\u7269\u8cea\u7684\u55ae\u4e00\u4f86\u6e90\uff0cNIST SRM 2034 \u662f\u53ef\u8ffd\u6eaf\u7684\u53c3\u8003\u6a19\u6e96\u3002<\/p><\/div><\/div>\n<div class=\"csg-faq-item\"><div class=\"csg-faq-q\">\u7a3d\u6838\u54e1\u671f\u671b\u770b\u5230\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\u7684\u54ea\u4e9b\u7d00\u9304\uff1f<\/div><div class=\"csg-faq-a\"><p>\u4e00\u4efd\u53c3\u7167\u9069\u7528\u85e5\u5178\u65b9\u6cd5\u7248\u672c\u7684 SOP\u3002\u6bcf\u500b\u9a57\u8b49\u6d3b\u52d5\u6709\u7c3d\u540d\u8207\u65e5\u671f\u7684\u9a57\u8b49\u7d00\u9304\u3002\u53c3\u8003\u7269\u8cea\u7684\u5206\u6790\u8b49\u66f8\u542b\u6279\u865f\u3001\u6709\u6548\u671f\u8207 NIST \u53ef\u8ffd\u6eaf\u6027\u3002\u986f\u793a\u904e\u53bb 6 \u5230 12 \u500b\u6708\u6b77\u53f2\u8868\u73fe\u7684\u8da8\u52e2\u5206\u6790\u3002\u4f9b\u61c9\u5546\u7dad\u4fee\u7d00\u9304\u3002\u5e8f\u865f\u8207\u4f7f\u7528\u4e2d\u6bd4\u8272\u76bf\u5c0d\u61c9\u7684\u6bd4\u8272\u76bf CoA\u3002\u4efb\u4f55\u9a57\u8b49\u5931\u6557\u7684\u8abf\u67e5\u5831\u544a\u542b\u6839\u56e0\u8207\u77ef\u6b63\u63aa\u65bd\u3002\u6bcf\u4efd\u7d00\u9304\u4e0a\u7684\u7a3d\u6838\u8ecc\u8de1\u7c3d\u540d\uff08\u64cd\u4f5c\u8005\u8207\u5be9\u6838\u8005\uff09\u662f\u6b63\u5f0f\u7684\u5408\u898f\u8981\u7d20\u3002<\/p><\/div><\/div>\n<\/div><h2 id=\"references\">15. \u6b0a\u5a01\u53c3\u8003\u6587\u737b<\/h2>\n<ul>\n<li>\u7f8e\u570b\u85e5\u5178\uff08USP\uff09\u3002\u901a\u5247 <851> <em>\u5206\u5149\u5149\u5ea6\u6cd5\u8207\u5149\u6563\u5c04<\/em>\u3002\u7f8e\u570b\u88fd\u85e5\u5206\u6790\u4e2d\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\u7684\u4e3b\u8981\u53c3\u8003\u3002<\/li>\n<li>\u6b50\u6d32\u85e5\u5178\uff08EP\uff09\u3002\u7ae0\u7bc0 2.2.25 <em>\u7d2b\u5916\u8207\u53ef\u898b\u5438\u6536\u5206\u5149\u5149\u5ea6\u6cd5<\/em>\u3002USP <851> \u7684\u6b50\u6d32\u5354\u8abf\u7b49\u6548\u7248\u3002<\/li>\n<li>\u65e5\u672c\u85e5\u5178\uff08JP\uff09\u3002\u7ae0\u7bc0 2.24 <em>\u7d2b\u5916-\u53ef\u898b\u5206\u5149\u5149\u5ea6\u6cd5<\/em>\u3002\u65e5\u672c\u7684\u7b49\u6548\u7248\u3002<\/li>\n<li>\u4e2d\u570b\u85e5\u5178\uff08ChP\uff09\u3002\u7ae0\u7bc0 0401 <em>\u7d2b\u5916-\u53ef\u898b\u5206\u5149\u5149\u5ea6\u6cd5<\/em>\u3002\u4e2d\u570b\u7684\u7b49\u6548\u7248\u3002<\/li>\n<li>NIST \u6a19\u6e96\u53c3\u8003\u8cc7\u6599\u5eab 2034 \u2014 \u9225\u6c27\u5316\u7269\u6eb6\u6db2\u6ce2\u9577\u6a19\u6e96\u3002\u6ce2\u9577\u6821\u6b63\u7684 NIST \u53ef\u8ffd\u6eaf\u53c3\u8003\u3002<\/li>\n<li>NIST SRM 935a \u2014 \u7d50\u6676\u91cd\u927b\u9178\u9240\uff08\u6c27\u5316\u6ef4\u5b9a\u6a19\u6e96\uff09\u3002\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u7684 NIST \u53ef\u8ffd\u6eaf\u53c3\u8003\u3002<\/li>\n<li>NIST SRM 930e \u2014 \u4e2d\u6027\u5bc6\u5ea6\u73bb\u7483\u6ffe\u5149\u7247\u3002\u56fa\u5b9a AU \u503c\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\u9a57\u8b49\u7684\u56fa\u9ad4\u53c3\u8003\u3002<\/li>\n<li>ICH Q2(R1) <em>\u5206\u6790\u7a0b\u5e8f\u7684\u78ba\u6548<\/em>\u3002\u5efa\u7acb\u542b\u5206\u5149\u5149\u5ea6\u6cd5\u7684\u5206\u6790\u65b9\u6cd5\u78ba\u6548\u6846\u67b6\u3002<\/li>\n<li>ASTM E275 <em>\u63cf\u8ff0\u8207\u91cf\u6e2c\u7d2b\u5916\u8207\u53ef\u898b\u5206\u5149\u5149\u5ea6\u8a08\u6027\u80fd\u7684\u6a19\u6e96\u505a\u6cd5<\/em>\u3002ASTM \u5c0d\u5206\u5149\u5149\u5ea6\u8a08\u6027\u80fd\u8868\u5fb5\u7684\u5171\u8b58\u3002<\/li>\n<\/ul><h2 id=\"disclaimer\">16. \u514d\u8cac\u8072\u660e\u8207\u8a3b\u8a18<\/h2>\n<div class=\"csg-callout\">\n<p><strong>\u65b9\u6cd5\u6b0a\u5a01\u3002<\/strong> \u672c\u9801\u63cf\u8ff0\u7684\u9a57\u8b49\u898f\u7a0b\u662f\u64b0\u5beb\u6642\u5df2\u767c\u8868\u85e5\u5178\u65b9\u6cd5\u7684\u6458\u8981\u3002\u8981\u5f97\u5230\u5177\u7d04\u675f\u529b\u7684\u5408\u898f\uff0c\u6c38\u9060\u53c3\u7167\u9069\u7528\u85e5\u5178\u65b9\u6cd5\u7684\u7576\u524d\u5df2\u767c\u8868\u7248\u672c\uff08USP <851>\u3001EP 2.2.25\u3001JP 2.24\u3001ChP 0401 \u6216\u7576\u5730\u7b49\u6548\uff09\u3002\u672c\u6307\u5357\u8207\u5df2\u767c\u8868\u65b9\u6cd5\u6709\u51fa\u5165\u6642\uff0c\u4ee5\u5df2\u767c\u8868\u65b9\u6cd5\u70ba\u6e96\u3002<\/p>\n<p><strong>\u5bb9\u5dee\u8207\u63a5\u53d7\u6a19\u6e96<\/strong> \u8868\u4e2d\u6240\u793a\u662f\u5178\u578b\u85e5\u5178\u503c\u3002\u7279\u5b9a\u65b9\u6cd5\u3001\u5100\u5668\u985e\u5225\u6216\u5be6\u9a57\u5ba4\u54c1\u8cea\u7cfb\u7d71\u7684\u5177\u9ad4\u5bb9\u5dee\u53ef\u80fd\u4e0d\u540c\u3002\u4f60\u63a1\u7528\u7684\u5bb9\u5dee\u5fc5\u9808\u7b26\u5408\u4f60\u7684 SOP \u8207\u78ba\u6548\u898f\u7a0b\u6240\u898f\u5b9a\u7684\u3002<\/p>\n<p><strong>\u6cd5\u898f\u8108\u7d61\u3002<\/strong> \u672c\u9801\u63cf\u8ff0 UV-Vis \u5206\u5149\u5149\u5ea6\u6cd5\u7684\u4e00\u822c\u9a57\u8b49\u505a\u6cd5\u3002\u5b83\u4e0d\u662f\u6cd5\u5f8b\u6216\u6cd5\u898f\u5efa\u8b70\u3002\u5c0d FDA\u3001EMA\u3001PMDA\u3001NMPA \u6216\u5176\u4ed6\u6cd5\u898f\u6a5f\u95dc\u7684\u5408\u898f\u53d6\u6c7a\u65bc\u7ba1\u8f44\u5340\u3001\u7522\u54c1\u985e\u5225\u8207\u65e2\u6709\u7684\u5177\u9ad4\u54c1\u8cea\u7cfb\u7d71\u3002\u5408\u898f\u5efa\u8b70\u8acb\u8aee\u8a62\u4f60\u7684\u54c1\u4fdd\u5718\u968a\u8207\u6cd5\u898f\u5c08\u5bb6\u3002<\/p>\n<p><strong>\u5546\u6a19\u8072\u660e\u3002<\/strong> USP\u3001EP\u3001JP\u3001ChP\u3001NIST \u8207 SRM \u8b58\u5225\u78bc\u662f\u5404\u81ea\u6a19\u6e96\u6a5f\u69cb\u7684\u5546\u6a19\u3002Hellma\u3001Starna\u3001Reagecon\u3001Camspec \u8207\u5176\u4ed6\u4f9b\u61c9\u5546\u540d\u7a31\u662f\u5404\u81ea\u6240\u6709\u8005\u7684\u5546\u6a19\u3002\u63d0\u53ca\u50c5\u4f9b\u76f8\u5bb9\u6027\u8207\u65b9\u6cd5\u8108\u7d61\u4e4b\u7528\u3002<\/p>\n<p><strong>\u8cc7\u8a0a\u6642\u6548\uff1a<\/strong> \u6700\u5f8c\u5be9\u95b1\u65bc 2026 \u5e74 5 \u6708\u3002\u85e5\u5178\u65b9\u6cd5\u6703\u9031\u671f\u6027\u66f4\u65b0\uff1b\u4f7f\u7528\u524d\u67e5\u7576\u524d\u4fee\u8a02\u7248\u3002<\/p>\n<\/div><script type=\"application\/ld+json\">{\"@context\": \"https:\/\/schema.org\", \"@type\": \"Article\", \"headline\": \"Cuvette & Spectrophotometer Verification: USP <851>, EP & GMP Protocols\", \"description\": \"Practitioner guide to spectrophotometer verification per USP <851>, EP 2.2.25 and equivalent pharmacopoeias. Wavelength accuracy, absorbance accuracy, linearity, stray light, resolution + cuvette path length, pair matching, and frequency.\", \"author\": {\"@type\": \"Organization\", \"name\": \"MachinedQuartz\"}, \"publisher\": {\"@type\": \"Organization\", \"name\": \"MachinedQuartz\", \"url\": \"https:\/\/machinedquartz.com\"}, \"datePublished\": \"2026-01-12\", \"dateModified\": \"2026-01-14\", \"mainEntityOfPage\": \"https:\/\/machinedquartz.com\/zh-hant\/cuvette-spectrophotometer-verification-usp-ep\/\", \"inLanguage\": \"zh-Hant\"}<\/script><script type=\"application\/ld+json\">{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What is USP <851> and why does it matter?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"USP <851> Spectrophotometry and Light-Scattering is the United States Pharmacopeia chapter that defines the methodology and acceptance criteria for verifying UV-Vis spectrophotometers used in pharmaceutical analysis. It specifies wavelength accuracy testing (with holmium oxide perchlorate solution), absorbance accuracy testing (with potassium dichromate), photometric linearity, stray light limits, and resolution. Compliance is required for any spectrophotometer used in GMP-regulated pharmaceutical analysis in the US. EP 2.2.25, JP 2.24, and ChP 0401 are the equivalent methods in Europe, Japan, and China and are essentially harmonised.\"}}, {\"@type\": \"Question\", \"name\": \"\u505a GMP \u5de5\u4f5c\u6211\u591a\u4e45\u8981\u9a57\u8b49\u4e00\u6b21\u5206\u5149\u5149\u5ea6\u8a08\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"\u983b\u7387\u7531\u5be6\u9a57\u5ba4\u7684\u54c1\u8cea\u7cfb\u7d71\u8207\u91cf\u6e2c\u7684\u95dc\u9375\u6027\u6c7a\u5b9a\u3002\u5178\u578b GMP \u6642\u7a0b\uff1a\u6bcf\u65e5\u7528\u55ae\u9ede\u54c1\u7ba1\u6a19\u6e96\u505a\u7cfb\u7d71\u9069\u7528\u6027\uff1b\u6bcf\u9031\u7528\u9225\u73bb\u7483\u505a\u6ce2\u9577\u5feb\u67e5\uff1b\u6bcf\u6708\u7528 USP \u53c3\u8003\u6eb6\u6db2\u505a\u5b8c\u6574\u6ce2\u9577\u8207\u5438\u5149\u5ea6\u6e96\u78ba\u5ea6\uff1b\u6bcf\u5b63\u505a\u5149\u5ea6\u7dda\u6027\u8207\u89e3\u6790\u5ea6\uff1b\u6bcf\u5e74\u7531\u4f9b\u61c9\u5546\u6216\u7b2c\u4e09\u65b9\u7dad\u4fee\u505a\u5b8c\u6574 IQ\/OQ\u3002\u4f9d\u4f60\u5177\u9ad4\u7684\u54c1\u8cea\u7cfb\u7d71\u8981\u6c42\u8207\u5100\u5668\u5931\u6548\u53f2\u8abf\u6574\u3002\"}}, {\"@type\": \"Question\", \"name\": \"\u6211\u80fd\u7528\u9225\u73bb\u7483\u53d6\u4ee3\u9225\u6c27\u5316\u7269\u6eb6\u6db2\u55ce\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"For routine intermediate checks, yes. USP <851> recognises both. The solid holmium glass is convenient for daily or weekly quick-checks because no solution preparation is needed. For full pharmacopoeial validation (monthly or annual full verification), the liquid holmium oxide perchlorate solution is the primary reference because the peak positions are sharper and the certified values are NIST-traceable through SRM 2034. Both are accepted; the solid is faster, the liquid is more rigorous.\"}}, {\"@type\": \"Question\", \"name\": \"What is the wavelength accuracy tolerance per USP <851>?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"\u5728\u7b49\u65bc\u6216\u4f4e\u65bc 400 nm \u7684\u6ce2\u9577\uff08\u7d2b\u5916\u5340\uff09\u6b63\u8ca0 1 nm\uff0c\u5728\u9ad8\u65bc 400 nm \u7684\u6ce2\u9577\uff08\u53ef\u898b\u5340\uff09\u6b63\u8ca0 3 nm\u3002EP 2.2.25 \u7528\u985e\u4f3c\u7684\u5bb9\u5dee\u3002\u8981\u66f4\u56b4\u7684\u6ce2\u9577\u63a7\u5236\uff080.5 nm \u4ee5\u4e0b\uff09\uff0c\u9700\u8981\u5c08\u9580\u7684\u7814\u7a76\u5100\u5668\u8207\u65b9\u6cd5\uff0cSOP \u61c9\u898f\u5b9a\u66f4\u56b4\u7684\u5bb9\u5dee\u3002\"}}, {\"@type\": \"Question\", \"name\": \"\u6211\u9700\u8981\u9a57\u8b49\u6bd4\u8272\u76bf\u5149\u7a0b\u55ce\uff0c\u9084\u662f\u88fd\u9020\u5546 CoA \u5c31\u5920\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"\u5c0d\u9032\u6599\u6aa2\u9a57\u8207\u4f8b\u884c\u4f7f\u7528\uff0c\u53ea\u8981\u88fd\u9020\u5546 CoA \u8f09\u660e\u5149\u7a0b\u5728\u6b63\u8ca0 0.005 cm \u5bb9\u5dee\u5167\u3001\u4e14\u53ef\u8ffd\u6eaf\u5230\u6821\u6b63\u6a19\u6e96\uff0c\u5c31\u53ef\u63a5\u53d7\u3002\u5c0d\u9577\u671f\u4f7f\u7528\u4e2d\uff08GMP \u6bd4\u8272\u76bf\u901a\u5e38\u6bcf 1 \u5230 2 \u5e74\uff09\uff0c\u7528 K2Cr2O7 \u53c3\u8003\u6eb6\u6db2\u505a\u6717\u4f2f-\u6bd4\u723e\u7684\u81ea\u884c\u9a57\u8b49\uff0c\u6293\u5f97\u5230\u539f\u59cb CoA \u6355\u6349\u4e0d\u5230\u7684\u4f7f\u7528\u4e2d\u6f02\u79fb\u3002\u85e5\u5178\u5de5\u4f5c\uff0c\u5728 SOP \u88e1\u8a18\u9304\u9a57\u8b49\u505a\u6cd5\u3002\"}}, {\"@type\": \"Question\", \"name\": \"\u96d9\u5149\u675f\u5206\u5149\u5149\u5ea6\u8a08\u6211\u600e\u9ebc\u914d\u5c0d\u6bd4\u8272\u76bf\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"\u5169\u652f\u6bd4\u8272\u76bf\u90fd\u586b\u540c\u6a23\u7684\u7a7a\u767d\u6eb6\u5291\u3002\u4e00\u652f\u653e\u6a23\u54c1\u5149\u675f\u3001\u4e00\u652f\u653e\u53c3\u8003\u5149\u675f\u3002\u5f9e 200 \u5230 800 nm \u8dd1\u57fa\u7dda\u6383\u63cf\u3002\u91cf\u5230\u7684\u5438\u5149\u5ea6\u5728\u6bcf\u500b\u6ce2\u9577\u90fd\u61c9\u662f 0.000 \u6b63\u8ca0 0.005 AU\u3002\u4efb\u4f55\u4e00\u81f4\u7684\u504f\u5dee\u5c31\u662f\u914d\u5c0d\u4e0d\u5339\u914d\u3002\u53ef\u63a5\u53d7\u7684\u914d\u5c0d\u5728\u5de5\u4f5c\u5149\u8b5c\u4e0a delta-A \u5c0f\u65bc 0.005\u3002\u6bd4\u8272\u76bf\u88fd\u9020\u5546\u7684\u9810\u5339\u914d\u914d\u5c0d\u9644\u6709\u6587\u4ef6\u8a18\u8f09\u7684\u4e0d\u5339\u914d\u8f2a\u5ed3\u3001\u662f\u6700\u7c21\u55ae\u7684\u9014\u5f91\u3002\"}}, {\"@type\": \"Question\", \"name\": \"\u91cd\u927b\u9178\u9240\u5728 257 nm \u7684 A1% \u503c\u662f\u591a\u5c11\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"A1% (the absorbance of a 1% w\/v solution in a 10 mm cell) for K2Cr2O7 in 5 mM H2SO4 at 257 nm is 144.0 per USP <851>. At 235 nm it is 124.5. At 313 nm, 48.6. At 350 nm, 106.6. These are the certified values that experimental measurements must match within plus or minus 2 percent relative to pass absorbance accuracy verification.\"}}, {\"@type\": \"Question\", \"name\": \"\u6211\u7684\u5149\u8b5c\u5100\u6c92\u901a\u904e\u7532\u82ef\/\u5df1\u70f7\u89e3\u6790\u5ea6\u6e2c\u8a66\u662f\u4ec0\u9ebc\u610f\u601d\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It means the spectral bandwidth (SBW) of your monochromator is too wide to resolve the closely spaced toluene peaks at 269 and 266 nm. The A269\/A266 ratio falls below the USP <851> pass criterion of 1.5. Either the spectrometer is operating with too wide an SBW setting (reduce the SBW if your instrument allows), or the instrument's design SBW is too wide for the regulated method (the instrument may not be suitable for this class of work). For most pharma assays with broader peaks, this is rarely the limiting factor.\"}}, {\"@type\": \"Question\", \"name\": \"\u6211\u80fd\u81ea\u884c\u88fd\u5099\u9225\u6c27\u5316\u7269\u6eb6\u6db2\u55ce\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. The USP <851> method specifies preparation: 4 g of high-purity Ho2O3 dissolved in 100 mL of 1.4 M HClO4. The prepared solution should be filled into a stoppered 10 mm quartz cuvette and verified against published peak positions before use. In-house preparation requires high-purity (greater than 99.9%) holmium oxide and concentrated perchloric acid; document the preparation, expiry, and verification in the SOP. NIST SRM 2034 is the traceable reference standard if you want a single source for the parent material.\"}}, {\"@type\": \"Question\", \"name\": \"\u7a3d\u6838\u54e1\u671f\u671b\u770b\u5230\u5206\u5149\u5149\u5ea6\u8a08\u9a57\u8b49\u7684\u54ea\u4e9b\u7d00\u9304\uff1f\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"\u4e00\u4efd\u53c3\u7167\u9069\u7528\u85e5\u5178\u65b9\u6cd5\u7248\u672c\u7684 SOP\u3002\u6bcf\u500b\u9a57\u8b49\u6d3b\u52d5\u6709\u7c3d\u540d\u8207\u65e5\u671f\u7684\u9a57\u8b49\u7d00\u9304\u3002\u53c3\u8003\u7269\u8cea\u7684\u5206\u6790\u8b49\u66f8\u542b\u6279\u865f\u3001\u6709\u6548\u671f\u8207 NIST \u53ef\u8ffd\u6eaf\u6027\u3002\u986f\u793a\u904e\u53bb 6 \u5230 12 \u500b\u6708\u6b77\u53f2\u8868\u73fe\u7684\u8da8\u52e2\u5206\u6790\u3002\u4f9b\u61c9\u5546\u7dad\u4fee\u7d00\u9304\u3002\u5e8f\u865f\u8207\u4f7f\u7528\u4e2d\u6bd4\u8272\u76bf\u5c0d\u61c9\u7684\u6bd4\u8272\u76bf CoA\u3002\u4efb\u4f55\u9a57\u8b49\u5931\u6557\u7684\u8abf\u67e5\u5831\u544a\u542b\u6839\u56e0\u8207\u77ef\u6b63\u63aa\u65bd\u3002\u6bcf\u4efd\u7d00\u9304\u4e0a\u7684\u7a3d\u6838\u8ecc\u8de1\u7c3d\u540d\uff08\u64cd\u4f5c\u8005\u8207\u5be9\u6838\u8005\uff09\u662f\u6b63\u5f0f\u7684\u5408\u898f\u8981\u7d20\u3002\"}}], \"inLanguage\": \"zh-Hant\"}<\/script><script type=\"application\/ld+json\">{\"@context\": \"https:\/\/schema.org\", \"@type\": \"BreadcrumbList\", \"itemListElement\": [{\"@type\": \"ListItem\", \"position\": 1, \"name\": \"Home\", \"item\": \"https:\/\/machinedquartz.com\"}, {\"@type\": \"ListItem\", \"position\": 2, \"name\": \"Knowledge Base\", \"item\": \"https:\/\/machinedquartz.com\/blog\/\"}, {\"@type\": \"ListItem\", \"position\": 3, \"name\": \"Cuvette & Spectrophotometer Verification\", \"item\": \"https:\/\/machinedquartz.com\/zh-hant\/cuvette-spectrophotometer-verification-usp-ep\/\"}], \"inLanguage\": \"zh-Hant\"}<\/script><script>\ndocument.querySelectorAll('.csg-faq-q').forEach(function(q) {\n  q.addEventListener('click', function() {\n    var item = this.closest('.csg-faq-item');\n    var isOpen = item.classList.contains('open');\n    document.querySelectorAll('.csg-faq-item').forEach(function(i){ i.classList.remove('open'); 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