Quartz 10mm Stoppered Micro Fluorescence Cuvette, 1.75 ml, Sintered 83, Four-Way Light, PTFE Stopper, 1pc/ea, C104SR21

Description

Fluorescence Applications

Why Four-Way Light for Fluorescence?

Fluorescence spectroscopy requires excitation light to enter through one face and emission to be collected at 90° through an adjacent face — the four-way configuration provides four polished optical windows that enable this standard geometry from any orientation. This also accommodates dual-emission or dual-excitation setups where detectors are positioned on opposite faces. All four windows are polished to spectroscopic grade, minimizing scatter-induced background that would otherwise raise detection limits.

Why PTFE Stopper?

The PTFE stopper provides a secure, chemical-resistant seal that prevents solvent evaporation and protects samples from atmospheric contamination. PTFE is inert to virtually all laboratory chemicals, making this stopper suitable for use with aqueous buffers, organic solvents, and air-sensitive fluorophores that degrade on contact with atmospheric oxygen. The stopper enables safe inversion for gentle mixing without leaks, and maintains stable fluorophore concentrations during extended kinetic measurements where even minor evaporation would shift emission intensity.

Why Sintered 83 Construction?

Sintered 83 cuvettes are assembled by fusing quartz panels at approximately 600 °C, creating strong, adhesive-free joints. With >83% transmittance across 190–2500 nm, Sintered 83 delivers excellent optical clarity. For fluorescence work, the absence of adhesive is critical — optical cements can auto-fluoresce under UV excitation, adding unwanted background signal. The fused joints are compatible with strong acids, bases, and most organic solvents (excluding HF and aggressive organics such as toluene and acetone).