Quartz 10mm Cuvette With Stopper For Ultra-Micro Volume, 200 µl, Standard 80, Two-Way Light, Stopper, 1pc/ea, C102SD2

Description

Why PTFE Stopper?

The PTFE stopper provides a secure, chemical-resistant seal that prevents solvent evaporation and protects samples from atmospheric contamination. PTFE (polytetrafluoroethylene) is inert to virtually all laboratory chemicals, making this stopper suitable for use with aqueous buffers, mild organic solvents, and air-sensitive samples. The stopper enables safe inversion for gentle mixing without leaks, and maintains stable sample concentrations during extended kinetic measurements where even minor evaporation would compromise results.

Applications

Why Ultra-Micro?

A raised quartz platform elevates 200 µl of sample precisely into the spectrophotometer beam at Z = 15 mm, saving 94% compared to a standard 3.5 ml cell while maintaining the full 10 mm optical path (Beer–Lambert law: A = εlc). This is essential for precious biological samples, limited column fractions, or expensive synthetic compounds. Important: the Z dimension must match your instrument’s beam center height — verify before ordering.

Black-Wall Design Benefits

This cuvette features a black-wall (dark-wall) exterior on the two non-optical faces. The opaque coating absorbs stray ambient light and eliminates fluorescence interference from scattered excitation photons bouncing off internal walls. Benefits include: reduced stray-light artifacts at low absorbance readings, improved signal-to-noise in fluorescence measurements, cleaner baselines in the deep-UV region (<230 nm) where scattered light is most problematic, and sharper spectral resolution in high-sensitivity absorbance work. If your application does not require lateral light transmission (excitation/emission at 90°), the black-wall two-way configuration delivers measurably better optical performance than an all-clear four-way cuvette.

Why Standard 80 Construction?

Standard 80 refers to precision-glued assembly: individual quartz panels bonded with optical-grade adhesive, then ground and polished to final dimensions. With >80% transmittance across 190–2500 nm, Standard 80 delivers accurate, reproducible results for routine UV-Vis work — DNA/protein quantification, enzyme kinetics, and pharmaceutical analysis. Important: the adhesive bonds are designed for aqueous samples only (water, buffers, dilute salt solutions). Organic solvents, strong acids, strong bases, and corrosive reagents can attack and degrade the adhesive joints. For work with organic solvents or aggressive chemicals, choose Sintered 83 or Molded 83 instead.